Doublecortin antibody - 326 017

Doublecortin or DCX is a protein associated with neuronal differentiation

Rat monoclonal purified IgG

Cat. No.: 326 017

Amount: 100 µg

Price: $420.00

Cat. No. 326 017	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP-staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	SY-179B7
Subtype	IgG2b (κ light chain)
Immunogen	Synthetic peptide corresponding to residues near the carboxy terminus of mouse Doublecortin (UniProt Id: Q6PGI2)
Reactivity	Reacts with: mouse (O88809), rat (Q9ESI7). Other species not tested yet. Predicted to cross-react with human (O43602) due to high sequence homology.
Remarks	IHC: Antigen retrieval with citrate buffer pH 6 is tolerated.
Data sheet	326_017.pdf

Cat. No.: 326 017

Amount: 100 µg

Price: $420.00

ICC

IHC

IHC-P

Background

Doublecortin or DCX seems to be required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. Mutations in the gene encoding this protein have been linked to the double cortex syndrome.
Doublecortin is highly expressed in neuronal cells of fetal brain but absent from other tissues. In the adult dentate gyrus it is only expressed in cells contributing to adult neurogenesis.

1 ) Tang SK et al., Neuroscience (2014)
2 ) Plümpe T et al., BMC Neurosci (2006)
3 ) Moores CA et al., EMBO J. (2006)
4 ) Francis F et al., Neuron (1999)
5 ) Gleeson JG et al., Cell (1998)

Protocols

ICC

IHC

IHC-P

Other products for this target