Tailor-made Antibodies
and Tools for Life Science

MLC-2A antibody - 311 011AT647N

Myosin light chain 2A or MLC-2A is specific for the atrium of the mammalian heart
Mouse monoclonal purified IgG
Cat. No.: 311 011AT647N
Amount: 100 µg
Price: $470.00
Cat. No. 311 011AT647N 100 µg purified IgG, lyophilized, fluorescence-labeled with ATTO® 647N.
Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: N/A
IP: N/A
ICC: yes (see remarks)
IHC: yes
IHC-P: 1 : 200 gallery  
Label ATTO 647N
Clone 56F5
Subtype IgG2b (κ light chain)
Immunogen Full-length recombinant human MLC-2A (UniProt Id: Q01449)
Reactivity Reacts with: human (Q01449), rat, mouse (Q9QVP4).
No signal: chicken.
Other species not tested yet.
Specificity Specific for MLC-2A, no cross-reactivity to MLC-2V.
Matching control protein/peptide 311-0P
Remarks

ICC: Methanol fixation gives stronger signals.

Data sheet 311_011at647n.pdf

References for MLC-2A - 311 011AT647N

Ascorbic acid induces MLC2v protein expression and promotes ventricular-like cardiomyocyte subtype in human induced pluripotent stem cells derived cardiomyocytes.
Gao Y, Su L, Wei Y, Tan S, Hu Z, Tao Z, Kovalik JP, Soong TW, Zhang J, Pu J, Ye L, et al.
Theranostics (2023) 1311: 3872-3896. 311 011AT647N ICC, FACS; tested species: human
Cat. No.: 311 011AT647N
Amount: 100 µg
Price: $470.00