Synaptic Systems - beta3-Tubulin

Cat. No.: 302 311

Amount: 100 µg

Price: $415.00

WB

ICC

IHC

IHC-P

Background

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintainance of cell shape (1).
Tubulin itself is a globular protein which consists of two polypeptides, α-tubulin and β-tubulin. α- and β-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 25 nm diameter (1).
Class III β-tubulin is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development (2).
It is widely used as a neuronal marker in normal and neoplastic tissues but has also been reported to be expressed in certain tumours of non-neuronal origin (3).

1 ) Katsetos CD et al., J. Child Neurol. (2003)
2 ) Katsetos CD et al., Cell Motil. Cytoskeleton (2003)
3 ) Dráberová E et al., Histochem. Cell Biol. (1998)
4 ) Koh Y et al., J Thorac Oncol (2010)
5 ) Jouhilahti EM et al., J. Histochem. Cytochem. (2008)
6 ) Sharma RK et al., Brain Res. (2007)
7 ) Stagi M et al., J. Neurosci. (2005)
8 ) Duittoz AH et al., J. Neurosci. Methods (2001)
9 ) Ferreira A et al., J. Neurosci. Res. (1992)
10 ) Lee MK et al., Proc. Natl. Acad. Sci. U.S.A. (1990)

Protocols

WB

IP

Standard protocol

ICC

IHC

IHC-P

OTHER

Preabsorbtion

Other products for this target

Cat. No. 302 311	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 5000 up to 1 : 10000 gallery IP: yes ICC: 1 : 500 up to 1 : 2000 gallery IHC: 1 : 5000 up to 1 : 10000 gallery IHC-P: 1 : 2000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	356E3D10
Subtype	IgG2a (κ light chain)
Immunogen	Synthetic peptide corresponding to residues near the carboxy terminus of mouse β3-tubulin (UniProt Id: Q9ERD7)
Reactivity	Reacts with: rat (Q4QRB4), mouse (Q9ERD7). Other species not tested yet.
Specificity	Specific for β3-tubulin.
Matching control protein/peptide	302-3P
Data sheet	302_311.pdf

beta3-Tubulin antibody - 302 311