Synaptic Systems - beta3-Tubulin

Cat. No.: 302 309

Amount: 50 µg

Price: $420.00

WB

ICC

IHC

IHC-P

Background

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintainance of cell shape (1).
Tubulin itself is a globular protein which consists of two polypeptides, α-tubulin and β-tubulin. α- and β-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 25 nm diameter (1).
Class III β-tubulin is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development (2).
It is widely used as a neuronal marker in normal and neoplastic tissues but has also been reported to be expressed in certain tumors of non-neuronal origin (3).

1 ) Katsetos CD et al., J. Child Neurol. (2003)
2 ) Katsetos CD et al., Cell Motil. Cytoskeleton (2003)
3 ) Dráberová E et al., Histochem. Cell Biol. (1998)
4 ) Koh Y et al., J Thorac Oncol (2010)
5 ) Jouhilahti EM et al., J. Histochem. Cytochem. (2008)
6 ) Sharma RK et al., Brain Res. (2007)
7 ) Stagi M et al., J. Neurosci. (2005)
8 ) Duittoz AH et al., J. Neurosci. Methods (2001)
9 ) Ferreira A et al., J. Neurosci. Res. (1992)
10 ) Lee MK et al., Proc. Natl. Acad. Sci. U.S.A. (1990)

Protocols

WB

ICC

IHC

IHC-P

OTHER

Preabsorbtion

Other products for this target

Cat. No. 302 309	50 µg purified recombinant IgY, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP-staining) gallery IP: not tested yet ICC: 1 : 1000 gallery IHC: 1 : 1000 up to 1 : 5000 gallery IHC-P: 1 : 1000 up to 1 : 4000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Ch356E3
Subtype	IgY (λ light chain)
Immunogen	Synthetic peptide corresponding to residues near the carboxy terminus of mouse β3-tubulin (UniProt Id: Q9ERD7)
Reactivity	Reacts with: rat (Q4QRB4), mouse (Q9ERD7). Other species not tested yet.
Matching control protein/peptide	302-3P
Remarks	This antibody is a chimeric antibody based on the monoclonal mouse antibody 356E3D10. The constant regions of the heavy and light chains have been replaced by chicken specific sequences. The antibody can therefore be used with standard anti-chicken secondary reagents. The antibody has been expressed in mammalian cells.
Data sheet	302_309.pdf

beta3-Tubulin antibody - 302 309