Cat. No.: 284 011
Amount: 100 µg
Price:
$420.00
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| Cat. No. 284 011 |
100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications | |
| Clone | SY-219D7 |
| Subtype | IgG2a (λ light chain) |
| Immunogen | Synthetic peptide corresponding to residues near the amino terminus of rat DAT (UniProt Id: P23977) |
| Reactivity |
Reacts with: mouse (Q01959), rat (P23977). Other species not tested yet. |
| Data sheet | 284_011.pdf |
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The dopamine transporter (DAT), encoded by the SLC6A3 gene, plays a crucial role in regulating dopaminergic neurotransmission by facilitating the reuptake of dopamine (DA) from the synaptic cleft back into presynaptic neurons (1, 2). Structurally, DAT consists of 12 transmembrane domains with interspersed extracellular and intracellular loops. The N- and C-terminal regions of the protein are intracellular, providing multiple potential phosphorylation sites that regulate its activity (1, 3).
Functionally, DAT contributes to the termination of DA signaling by reuptaking DA, thereby controlling both the duration and magnitude of dopaminergic transmission (4). It is predominantly found in dopaminergic neurons and enriched in regions such as the striatum, substantia nigra, and ventral tegmental area, which are associated with motor control and reward mechanisms (5).
Alterations in DAT expression have been linked to various neuropsychiatric disorders, including Parkinson's disease and schizophrenia, emphasizing its importance in maintaining dopaminergic homeostasis and neuronal integrity (6, 7).