DAT antibody - 284 006

DAT is a neurotransmitter transporter involved in the termination of dopamine transmission

Chicken polyclonal purified antibody

Cat. No.: 284 006

Amount: 50 µg

Price: $380.00

For the import of chicken antibodies into the USA you need a special import permit. This can be applied for at your USDA office. If you cannot apply for this, please order the chicken antibodies through our distributor Cedarlane USA. If you have any questions, please contact us.

Cat. No. 284 006	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: not tested yet IHC: 1 : 500 gallery IHC-P: 1 : 500 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 7 to 22 from rat DAT (UniProt Id: P23977)
Reactivity	Reacts with: mouse (Q01959), rat (P23977). Other species not tested yet.
Remarks	IHC-P: Requires heat mediated antigen retrieval with 10 mM Tris pH 9.0, 1 mM EDTA and 0.05 % Tween 20.
Data sheet	284_006.pdf

Cat. No.: 284 006

Amount: 50 µg

Price: $380.00

IHC

IHC-P

Background

During neurotransmission neurotransmitters like GABA, glycine, glutamate, dopamine, etc. are released from synaptic vesicles into the synaptic cleft. The dopamine active transporter (DAT) translocates the neurotransmitter dopamine back into the cytoplasm of the neuron.

1 ) Cone JJ et al., PLoS ONE (2013)
2 ) Egaña LA et al., J. Neurosci. (2009)
3 ) Nirenberg MJ et al., J. Neurosci. (1996)
4 ) Thibaut F et al., Encephale ()
5 ) Kuhar MJ et al., Eur. Neurol. (1990)

Protocols

IHC

IHC-P

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