Synaptic Systems - GABA transporter3

Cat. No.: 274 304

Amount: 100 µl

Price: $365.00

Cat. No. 274 304	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 500 gallery ExM: yes Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Expansion Microscopy (ExM) is a sample preparation and imaging method which employs a dense interconnected web of swellable polymer within a biological specimen. A significantly higher effective resolution can be achieved with a standard microscope setup. For additional experimental details, please refer to the Remarks section.
Immunogen	Synthetic peptide corresponding to AA 612 to 627 from mouse GABA transporter3 (UniProt Id: P31650)
Reactivity	Reacts with: rat (P31647), mouse (P31650). Other species not tested yet.
Matching control protein/peptide	274-3P
Remarks	WB: GABA transporter 3 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Data sheet	274_304.pdf

References for GABA transporter3 - 274 304

Extracellular Vesicles from Mesenchymal Stem Cells Reverse Neuroinflammation and Restore Motor Coordination in Hyperammonemic Rats.
Izquierdo-Altarejos P, Martínez-García M, Atienza-Pérez I, Hernández A, Moreno-Manzano V, Llansola M, Felipo V
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology (2024) 191: 52. 274 304 WB; tested species: rat

Extracellular Vesicles from Hyperammonemic Rats Induce Neuroinflammation and Motor Incoordination in Control Rats.
Izquierdo-Altarejos P, Cabrera-Pastor A, Gonzalez-King H, Montoliu C, Felipo V
Cells (2020) 93: . 274 304 WB; tested species: rat

Neurons Induce Tiled Astrocytes with Branches That Avoid Each Other.
Hayashi MK, Sato K, Sekino Y
International journal of molecular sciences (2022) 238: . 274 304 ICC; tested species: rat

Light microscopic and heterogeneity analysis of astrocytes in the common marmoset brain.
Muñoz Y, Cuevas-Pacheco F, Quesseveur G, Murai KK
Journal of neuroscience research (2021) : . 274 304 IHC; tested species: marmoset

Doublecortin-like expressing astrocytes of the suprachiasmatic nucleus are implicated in the biosynthesis of vasopressin and influences circadian rhythms.
Coomans C, Saaltink DJ, Deboer T, Tersteeg M, Lanooij S, Schneider AF, Mulder A, van Minnen J, Jost C, Koster AJ, Vreugdenhil E, et al.
Glia (2021) 6911: 2752-2766. 274 304 IHC; tested species: mouse

Astrocytes detect and upregulate transmission at inhibitory synapses of somatostatin interneurons onto pyramidal cells.
Matos M, Bosson A, Riebe I, Reynell C, Vallée J, Laplante I, Panatier A, Robitaille R, Lacaille JC
Nature communications (2018) 91: 4254. 274 304 IHC; tested species: mouse

Olig2-Lineage Astrocytes: A Distinct Subtype of Astrocytes That Differs from GFAP Astrocytes.
Tatsumi K, Isonishi A, Yamasaki M, Kawabe Y, Morita-Takemura S, Nakahara K, Terada Y, Shinjo T, Okuda H, Tanaka T, Wanaka A, et al.
Frontiers in neuroanatomy (2018) 12: 8. 274 304 IHC; tested species: mouse

Structural Heterogeneity of the GABAergic Tripartite Synapse.
Brunskine C, Passlick S, Henneberger C
Cells (2022) 1119: . 274 304 ExM; tested species: mouse

Cat. No.: 274 304

Amount: 100 µl

Price: $365.00

Neurons Induce Tiled Astrocytes with Branches That Avoid Each Other.
Hayashi MK, Sato K, Sekino Y
International journal of molecular sciences (2022) 238: . 274 304 ICC; tested species: rat

Structural Heterogeneity of the GABAergic Tripartite Synapse.
Brunskine C, Passlick S, Henneberger C
Cells (2022) 1119: . 274 304 ExM; tested species: mouse

ICC

IHC

IHC-P

Background

γ-aminobutyric acid (GABA) is a major inhibitory neurotransmitter. After the release of GABA from synaptic vesicles into the synaptic cleft during neurotransmission, GABA transporters (GATs) remove extracellular GABA by reuptake into the presynaptic terminal.
Three GABA transporters are described so far of which only GAT 1 and GAT 3 are expressed in the brain.

1 ) Hu J et al., Neuropharmacology (2008)
2 ) Quick MW et al., J. Biol. Chem. (2004)
3 ) Law RM et al., J. Biol. Chem. (2000)
4 ) Beckman ML et al., J. Neurosci. (1998)
5 ) Ikegaki N et al., Brain Res. Mol. Brain Res. (1994)
6 ) Jursky F et al., J. Exp. Biol. (1994)

Protocols

Standard protocol

ICC

IHC

IHC-P

OTHER

Preabsorbtion

Other products for this target

GABA transporter3 antibody - 274 304

References for GABA transporter3 - 274 304