Synaptic Systems - GABA transporter3

Cat. No.: 274 303

Amount: 50 µg

Price: $455.00

Rifaximin Prevents T-Lymphocytes and Macrophages Infiltration in Cerebellum and Restores Motor Incoordination in Rats with Mild Liver Damage.
Balzano T, Leone P, Ivaylova G, Castro MC, Reyes L, Ramón C, Malaguarnera M, Llansola M, Felipo V
Biomedicines (2021) 98: . 274 303 WB; tested species: rat

WB

ICC

IHC

IHC-P

Background

γ-aminobutyric acid (GABA) is a major inhibitory neurotransmitter. After the release of GABA from synaptic vesicles into the synaptic cleft during neurotransmission, GABA transporters (GATs) remove extracellular GABA by reuptake into the presynaptic terminal.
Three GABA transporters are described so far of which only GAT 1 and GAT 3 are expressed in the brain.

1 ) Hu J et al., Neuropharmacology (2008)
2 ) Quick MW et al., J. Biol. Chem. (2004)
3 ) Law RM et al., J. Biol. Chem. (2000)
4 ) Beckman ML et al., J. Neurosci. (1998)
5 ) Ikegaki N et al., Brain Res. Mol. Brain Res. (1994)
6 ) Jursky F et al., J. Exp. Biol. (1994)

Protocols

WB

ICC

IHC

IHC-P

OTHER

Preabsorbtion

Other products for this target

Cat. No. 274 303	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 up to 1 : 1000 gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 612 to 627 from mouse GABA transporter3 (UniProt Id: P31650)
Reactivity	Reacts with: rat (P31647), mouse (P31650). Other species not tested yet.
Matching control protein/peptide	274-3P
Remarks	WB: GABA transporter 3 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Data sheet	274_303.pdf

GABA transporter3 antibody - 274 303

References for GABA transporter3 - 274 303