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NeuN antibody - 266 014

NeuN is a neuron specific nuclear protein in vertebrates
Guinea pig polyclonal antiserum
Cat. No.: 266 014
Amount: 100 µl
Price: $375.00
Cat. No. 266 014 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not tested yet
IP: not tested yet
ICC: 1 : 2000 up to 1 : 5000 gallery  
IHC: 1 : 2000 gallery  
IHC-P: 1 : 2000 up to 1 : 4000 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to residues near the amino terminus of human NeuN (UniProt Id: A6NFN3)
Reactivity Reacts with: human (A6NFN3), mouse (Q8BIF2), rat (D4A2H6).
Other species not tested yet.
Data sheet 266_014.pdf

References for NeuN - 266 014

Temporal characterisation and electrophysiological implications of TBI-induced serine/threonine kinase activity in mouse cortex.
Celine G, Thomas M
Cellular and molecular life sciences : CMLS (2025) 821: 102. 266 014 WB, IHC; tested species: mouse
Temporal characterisation and electrophysiological implications of TBI-induced serine/threonine kinase activity in mouse cortex.
Celine G, Thomas M
Cellular and molecular life sciences : CMLS (2025) 821: 102. 266 014 WB, IHC; tested species: mouse
Cat. No.: 266 014
Amount: 100 µl
Price: $375.00
Temporal characterisation and electrophysiological implications of TBI-induced serine/threonine kinase activity in mouse cortex.
Celine G, Thomas M
Cellular and molecular life sciences : CMLS (2025) 821: 102. 266 014 WB, IHC; tested species: mouse
Temporal characterisation and electrophysiological implications of TBI-induced serine/threonine kinase activity in mouse cortex.
Celine G, Thomas M
Cellular and molecular life sciences : CMLS (2025) 821: 102. 266 014 WB, IHC; tested species: mouse
ICC
IHC
IHC
IHC-P
IHC-P
Background

NeuN (Neuronal Nuclei) is a neuron-specific nuclear protein that has been identified as Fox-3/Rbfox3, a member of the Fox-1 family of transcription factors.
NeuN is only expressed in the nuclei of differentiated neurons. In some neurons - Purkinje cells, sympathetic ganglion cells, INL retinal cells, Cajal-Retzius cells, inferior olivary, and dentate nucleus neurons - NeuN is not detectable.
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