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NeuN antibody - 266 011

NeuN is a neuron specific nuclear protein in vertebrates
Mouse monoclonal purified IgG
Cat. No.: 266 011
Amount: 100 µg
Price: $420.00
Cat. No. 266 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not tested yet
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 1000 up to 1 : 5000      (see remarks) gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone 350D3
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 97 from mouse NeuN (UniProt Id: Q8BIF2)
Epitop AA 5 to 15 from mouse NeuN (UniProt Id: Q8BIF2)
Reactivity Reacts with: rat (D4A2H6), mouse (Q8BIF2).
No signal: human (A6NFN3).
Other species not tested yet.
Remarks

IHC-P: For best results apply mild antigen retrieval 
Data sheet 266_011.pdf

References for NeuN - 266 011

Extracellular matrix remodeling through endocytosis and resurfacing of Tenascin-R.
Dankovich TM, Kaushik R, Olsthoorn LHM, Petersen GC, Giro PE, Kluever V, Agüi-Gonzalez P, Grewe K, Bao G, Beuermann S, Hadi HA, et al.
Nature communications (2021) 121: 7129. 266 011 ICC; tested species: rat
In vivo neuronal and astrocytic activation in somatosensory cortex by acupuncture stimuli.
Chang XY, Chen K, Cheng T, Lai PT, Zhang L, So KF, Yang ES
Neural regeneration research (2022) 1711: 2526-2529. 266 011 IHC; tested species: mouse
Ventral hippocampal projections to the medial prefrontal cortex regulate social memory.
Phillips ML, Robinson HA, Pozzo-Miller L
eLife (2019) 8: . 266 011 IHC; tested species: mouse
Cat. No.: 266 011
Amount: 100 µg
Price: $420.00
Extracellular matrix remodeling through endocytosis and resurfacing of Tenascin-R.
Dankovich TM, Kaushik R, Olsthoorn LHM, Petersen GC, Giro PE, Kluever V, Agüi-Gonzalez P, Grewe K, Bao G, Beuermann S, Hadi HA, et al.
Nature communications (2021) 121: 7129. 266 011 ICC; tested species: rat
In vivo neuronal and astrocytic activation in somatosensory cortex by acupuncture stimuli.
Chang XY, Chen K, Cheng T, Lai PT, Zhang L, So KF, Yang ES
Neural regeneration research (2022) 1711: 2526-2529. 266 011 IHC; tested species: mouse
Ventral hippocampal projections to the medial prefrontal cortex regulate social memory.
Phillips ML, Robinson HA, Pozzo-Miller L
eLife (2019) 8: . 266 011 IHC; tested species: mouse
ICC
IHC
IHC-P
IHC-P
Background

NeuN (Neuronal Nuclei) is a neuron-specific nuclear protein that has been identified as Fox-3/Rbfox3, a member of the Fox-1 family of transcription factors.
NeuN is only expressed in the nuclei of differentiated neurons. In some neurons - Purkinje cells, sympathetic ganglion cells, INL retinal cells, Cajal-Retzius cells, inferior olivary, and dentate nucleus neurons - NeuN is not detectable.
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