Collybistin antibody - 261 111

Collybistin is a protein involved in gephyrin clustering

Mouse monoclonal purified IgG

Cat. No.: 261 111

Amount: 100 µg

Price: $415.00

Cat. No. 261 111	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: yes ICC: 1 : 500 gallery IHC: not tested yet IHC-P: not recommended Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	197A10
Subtype	IgG2b (κ light chain)
Immunogen	Recombinant protein corresponding to AA 1 to 471 from rat Collybistin (UniProt Id: Q9QX73)
Epitop	AA 4 to 229 from rat Collybistin (UniProt Id: Q9QX73)
Reactivity	Reacts with: rat (Q9QX73), mouse (Q3UTH8). Other species not tested yet.
Specificity	Specific for collybistin, immunogen present in all three described splice-variants.
Data sheet	261_111.pdf

Cat. No.: 261 111

Amount: 100 µg

Price: $415.00

ICC

Background

The GDP/GTP-exchange factor collybistin is composed of a dbl homology domain (DH) and a pleckstrin homology domain (PH) connected by a linker sequence. Three splice variants with different C-terminal regions have been described, so far.
Collybistin is supposed to be involved in the clustering of gephyrin, a scaffolding protein linking glycine and GABA receptors to microtubuli.

1 ) Papadopoulos T et al., Front Cell Neurosci (2011)
2 ) Saiepour L et al., J. Biol. Chem. (2010)
3 ) Jedlicka P et al., Mol. Cell. Neurosci. (2009)
4 ) Harvey K et al., J. Neurosci. (2004)
5 ) Kins S et al., Nat. Neurosci. (2000)

Protocols

Standard protocol

ICC

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