Synaptic Systems - Collybistin

Cat. No.: 261 003

Amount: 50 µg

Price: $375.00

Cat. No. 261 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 2000 gallery IP: yes ICC: 1 : 500 gallery IHC: yes IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 4 to 229 from rat Collybistin (UniProt Id: Q9QX73)
Reactivity	Reacts with: rat (Q9QX73), mouse (Q3UTH8). Other species not tested yet.
Specificity	Specific for collybistin; immunogen present in all three described splice-variants K.O. validated
Data sheet	261_003.pdf

References for Collybistin - 261 003

A proline-rich motif in the large intracellular loop of the glycine receptor α1 subunit interacts with the Pleckstrin homology domain of collybistin.
Breitinger U, Weinländer K, Pechmann Y, Langlhofer G, Enz R, Becker CM, Sticht H, Kneussel M, Villmann C, Breitinger HG
Journal of advanced research (2021) 29: 95-106. 261 003 WB, ICC; tested species: mouse

Phosphorylation on Ser 359 of the α2 subunit in GABA type A receptors down-regulates their density at inhibitory synapses.
Nakamura Y, Morrow DH, Nathanson AJ, Henley JM, Wilkinson KA, Moss SJ
The Journal of biological chemistry (2020) : . 261 003 WB; tested species: rat

Identification of a Core Amino Acid Motif within the α Subunit of GABAARs that Promotes Inhibitory Synaptogenesis and Resilience to Seizures.
Nathanson AJ, Zhang Y, Smalley JL, Ollerhead TA, Rodriguez Santos MA, Andrews PM, Wobst HJ, Moore YE, Brandon NJ, Hines RM, Davies PA, et al.
Cell reports (2019) 283: 670-681.e8. 261 003 WB, ICC; tested species: mouse

Collybistin binds and inhibits mTORC1 signaling: a potential novel mechanism contributing to intellectual disability and autism.
Machado CO, Griesi-Oliveira K, Rosenberg C, Kok F, Martins S, Passos-Bueno MR, Sertie AL
European journal of human genetics : EJHG (2016) 241: 59-65. 261 003 WB

Proteomic Characterization of Inhibitory Synapses Using a Novel pHluorin-tagged γ-Aminobutyric Acid Receptor, Type A (GABAA), α2 Subunit Knock-in Mouse.
Nakamura Y, Morrow DH, Modgil A, Huyghe D, Deeb TZ, Lumb MJ, Davies PA, Moss SJ
The Journal of biological chemistry (2016) 29123: 12394-407. 261 003 WB

Physical Interactions and Functional Relationships of Neuroligin 2 and Midbrain Serotonin Transporters.
Ye R, Quinlan MA, Iwamoto H, Wu HH, Green NH, Jetter CS, McMahon DG, Veestra-VanderWeele J, Levitt P, Blakely RD
Frontiers in synaptic neuroscience (2015) 7: 20. 261 003 WB

A conformational switch in collybistin determines the differentiation of inhibitory postsynapses.
Soykan T, Schneeberger D, Tria G, Buechner C, Bader N, Svergun D, Tessmer I, Poulopoulos A, Papadopoulos T, Varoqueaux F, Schindelin H, et al.
The EMBO journal (2014) 3318: 2113-33. 261 003 WB

Recruitment of Plasma Membrane GABA-A Receptors by Submembranous Gephyrin/Collybistin Clusters.
George S, Chiou TT, Kanamalla K, De Blas AL
Cellular and molecular neurobiology (2021) : . 261 003 ICC; tested species: mouse

Alternative Splicing and the Intracellular Domain Mediate TM-agrin's Ability to Differentially Regulate the Density of Excitatory and Inhibitory Synapse-like Specializations in Developing CNS Neurons.
Handara G, Kröger S
Neuroscience (2019) : . 261 003 ICC; tested species: mouse

Selective overexpression of Collybistin in mouse hippocampal pyramidal cells enhances GABAergic neurotransmission and protects against PTZ-induced seizures.
George S, James S, de Blas AL
eNeuro (2021) : . 261 003 IHC; tested species: mouse

In vivo transgenic expression of collybistin in neurons of the rat cerebral cortex.
Fekete CD, Goz RU, Dinallo S, Miralles CP, Chiou TT, Bear J, Fiondella CG, LoTurco JJ, De Blas AL
The Journal of comparative neurology (2017) 5255: 1291-1311. 261 003 IHC; tested species: rat

Gephyrin clusters are absent from small diameter primary afferent terminals despite the presence of GABA(A) receptors.
Lorenzo LE, Godin AG, Wang F, St-Louis M, Carbonetto S, Wiseman PW, Ribeiro-da-Silva A, De Koninck Y
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3424: 8300-17. 261 003 IHC

Cat. No.: 261 003

Amount: 50 µg

Price: $375.00

ICC

Background

The GDP/GTP-exchange factor collybistin is composed of a dbl homology domain (DH) and a pleckstrin homology domain (PH) connected by a linker sequence. Three splice variants with different C-terminal regions have been described, so far.
Collybistin is supposed to be involved in the clustering of gephyrin, a scaffolding protein linking glycine and GABA receptors to microtubuli.

1 ) Papadopoulos T et al., Front Cell Neurosci (2011)
2 ) Saiepour L et al., J. Biol. Chem. (2010)
3 ) Jedlicka P et al., Mol. Cell. Neurosci. (2009)
4 ) Harvey K et al., J. Neurosci. (2004)
5 ) Kins S et al., Nat. Neurosci. (2000)

Protocols

Standard protocol

ICC

IHC

Other products for this target

Collybistin antibody - 261 003 K.O.

References for Collybistin - 261 003