Synaptic Systems - Chromogranin B

Cat. No.: 259 103

Amount: 50 µg

Price: $375.00

Cat. No. 259 103	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 500 up to 1 : 10000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 407 to 677 from mouse Chromogranin B (UniProt Id: P16014)
Reactivity	Reacts with: rat (O35314), mouse (P16014). Other species not tested yet.
Specificity	Specific chromogranin B. K.O. validated PubMed: 29178418
Matching control protein/peptide	259-1P
Data sheet	259_103.pdf

References for Chromogranin B - 259 103

CaMKII controls neuromodulation via neuropeptide gene expression and axonal targeting of neuropeptide vesicles.
Moro A, van Woerden GM, Toonen RF, Verhage M
PLoS biology (2020) 188: e3000826. 259 103 WB, ICC; tested species: mouse

The RAB3-RIM Pathway Is Essential for the Release of Neuromodulators.
Persoon CM, Hoogstraaten RI, Nassal JP, van Weering JRT, Kaeser PS, Toonen RF, Verhage M
Neuron (2019) 1046: 1065-1080.e12. 259 103 WB, ICC; tested species: mouse

Dense-core vesicle biogenesis and exocytosis in neurons lacking chromogranins A and B.
Dominguez N, van Weering JRT, Borges R, Toonen RFG, Verhage M
Journal of neurochemistry (2018) 1443: 241-254. 259 103 WB; KO verified; tested species: mouse

High-throughput assay for regulated secretion of neuropeptides in mouse and human neurons.
Baginska U, Balagura G, Toonen RF, Verhage M
The Journal of biological chemistry (2024) : 107321. 259 103 ICC; tested species: mouse

Tomosyn affects dense core vesicle composition but not exocytosis in mammalian neurons.
Subkhangulova A, Gonzalez-Lozano MA, Groffen AJA, van Weering JRT, Smit AB, Toonen RF, Verhage M
eLife (2023) 12: . 259 103 ICC; tested species: mouse

Synaptotagmin 9 Modulates Spontaneous Neurotransmitter Release in Striatal Neurons by Regulating Substance P Secretion.
Seibert MJ, Evans CS, Stanley KS, Wu Z, Chapman ER
The Journal of neuroscience : the official journal of the Society for Neuroscience (2023) 439: 1475-1491. 259 103 ICC; tested species: mouse

Differential axonal trafficking of Neuropeptide Y-, LAMP1-, and RAB7-tagged organelles in vivo.
Nassal JP, Murphy FH, Toonen RF, Verhage M
eLife (2022) 11: . 259 103 ICC; tested species: mouse

Vti1a/b support distinct aspects of TGN and cis-/medial Golgi organization.
van Bommel DM, Toonen RF, Verhage M
Scientific reports (2022) 121: 20870. 259 103 ICC; tested species: mouse

The phosphoprotein Synapsin Ia regulates the kinetics of dense-core vesicle release.
Yang HJ, Chen PC, Huang CT, Cheng TL, Hsu SP, Chen CY, Lu JC, Wang CT
The Journal of neuroscience : the official journal of the Society for Neuroscience (2021) : . 259 103 ICC; tested species: rat

Dynamin controls neuropeptide secretion by organizing dense-core vesicle fusion sites.
Moro A, van Nifterick A, Toonen RF, Verhage M
Science advances (2021) 721: . 259 103 ICC; tested species: mouse

Fbxo41 Promotes Disassembly of Neuronal Primary Cilia.
King CR, A A Quadros AR, Chazeau A, Saarloos I, van der Graaf AJ, Verhage M, Toonen RF
Scientific reports (2019) 91: 8179. 259 103 ICC; tested species: mouse

Pool size estimations for dense-core vesicles in mammalian CNS neurons.
Persoon CM, Moro A, Nassal JP, Farina M, Broeke JH, Arora S, Dominguez N, van Weering JR, Toonen RF, Verhage M
The EMBO journal (2018) : . 259 103 ICC; tested species: mouse

SNAP-25 gene family members differentially support secretory vesicle fusion.
Arora S, Saarloos I, Kooistra R, van de Bospoort R, Verhage M, Toonen RF
Journal of cell science (2017) 13011: 1877-1889. 259 103 ICC; tested species: mouse

Tomosyn associates with secretory vesicles in neurons through its N- and C-terminal domains.
Geerts CJ, Mancini R, Chen N, Koopmans FTW, Li KW, Smit AB, van Weering JRT, Verhage M, Groffen AJA
PloS one (2017) 127: e0180912. 259 103 ICC; tested species: mouse

CAPS-1 promotes fusion competence of stationary dense-core vesicles in presynaptic terminals of mammalian neurons.
Farina M, van de Bospoort R, He E, Persoon CM, van Weering JR, Broeke JH, Verhage M, Toonen RF
eLife (2015) 4: . 259 103 ICC

The SNARE protein vti1a functions in dense-core vesicle biogenesis.
Walter AM, Kurps J, de Wit H, Schöning S, Toft-Bertelsen TL, Lauks J, Ziomkiewicz I, Weiss AN, Schulz A, Fischer von Mollard G, Verhage M, et al.
The EMBO journal (2014) 3315: 1681-97. 259 103 ICC; tested species: mouse

Single-cell transcriptomics of human embryos identifies multiple sympathoblast lineages with potential implications for neuroblastoma origin.
Kameneva P, Artemov AV, Kastriti ME, Faure L, Olsen TK, Otte J, Erickson A, Semsch B, Andersson ER, Ratz M, Frisén J, et al.
Nature genetics (2021) 535: 694-706. 259 103 IHC; tested species: mouse

Cat. No.: 259 103

Amount: 50 µg

Price: $375.00

Differential axonal trafficking of Neuropeptide Y-, LAMP1-, and RAB7-tagged organelles in vivo.
Nassal JP, Murphy FH, Toonen RF, Verhage M
eLife (2022) 11: . 259 103 ICC; tested species: mouse

Vti1a/b support distinct aspects of TGN and cis-/medial Golgi organization.
van Bommel DM, Toonen RF, Verhage M
Scientific reports (2022) 121: 20870. 259 103 ICC; tested species: mouse

ICC

IHC

IHC-P

Background

Chromogranin A (CgA) and B (CgB) are members of a family of acidic proteins stored and released throughout the neuroendocrine system. The large dense core vesicle associated proteins have multiple functions in neurons and neuroendocrine cells. They are differentially processed in different tissues.
Chromogranin A (CgA) is the precursor for the bioactive peptides pancreastatin, vasostatins, catestatin, β-granin and WE-14.

1 ) Domínguez N et al., J. Mol. Neurosci. (2012)
2 ) Park JJ et al., J. Cell. Sci. (2011)
3 ) Díaz-Vera J et al., J. Neurosci. (2010)
4 ) Schafer MK et al., Regul. Pept. (2010)
5 ) Machado JD et al., Cell. Mol. Neurobiol. (2010)
6 ) Montesinos MS et al., J. Neurosci. (2008)
7 ) Kim T et al., Cell (2001)
8 ) Natori S et al., EMBO J. (1998)
9 ) Kroesen S et al., Eur. J. Neurosci. (1996)
10 ) Wu HJ et al., J. Biol. Chem. (1991)

Protocols

ICC

IHC

IHC-P

OTHER

Preabsorbtion

Other products for this target

Chromogranin B antibody - 259 103 K.O.

References for Chromogranin B - 259 103