ClC3 antibody - 252 003

CLC3 is a chloride channel providing charge balance in acidic organelles

Rabbit polyclonal purified antibody

Cat. No.: 252 003

Amount: 50 µg

Price: $375.00

Cat. No. 252 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 616 to 747 from mouse ClC3 (UniProt Id: P51791)
Reactivity	Reacts with: rat (P51792), mouse (P51791). Other species not tested yet.
Specificity	Epitope present in both splice variants of ClC 3. May cross react to ClC 4 and 5, due to sequence homology.
Matching control protein/peptide	252-0P
Data sheet	252_003.pdf

References for ClC3 - 252 003

CAPS1 effects on intragranular pH and regulation of BDNF release from secretory granules in hippocampal neurons.
Eckenstaler R, Lessmann V, Brigadski T
Journal of cell science (2016) 1297: 1378-90. 252 003 ICC; tested species: rat

Cat. No.: 252 003

Amount: 50 µg

Price: $375.00

Background

The ClC 3 chloride channel, also referred to as ClCN 3, belongs to the voltage-gated chloride channels and is ubiquitously expressed throughout the brain and other tissues. Two splice variants of the protein have been described. ClC 3 resides on intracellular trafficking vesicles including synaptic vesicles. It mediates the exchange of chloride ions against protons. In contrast to the translocation of glutamate into synaptic vesicles, the VGAT mediated transport of GABA requires ClC 3. It has been postulated that ClC 3 provides chloride ions as charge balance during the acidification of the vesicle lumen by proton pumping of the V-ATPase.

1 ) Riazanski V et al., Nat. Neurosci. (2011)
2 ) Cuddapah VA et al., J. Biol. Chem. (2010)
3 ) Salazar G et al., J. Biol. Chem. (2004)
4 ) Olsen ML et al., J. Neurosci. (2003)
5 ) Li X et al., Am. J. Physiol., Cell Physiol. (2002)
6 ) Huang P et al., J. Biol. Chem. (2001)

Protocols

OTHER

Preabsorbtion

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