Synaptic Systems - beta-Actin

Cat. No.: 251 011

Amount: 100 µg

Price: $420.00

Cat. No. 251 011	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery IP: yes ICC: 1 : 500 (see remarks) gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	130B4
Subtype	IgG1 (κ light chain)
Immunogen	Synthetic peptide corresponding to AA 2 to 16 from mouse β-Actin (UniProt Id: P60710)
Reactivity	Reacts with: rat (P60711), mouse (P60710), zebrafish, human (P60709). Other species not tested yet.
Specificity	May cross-react to α- and γ-actin due to sequence homology.
Remarks	ICC: methanol or PFA fixation
Data sheet	251_011.pdf

References for beta-Actin - 251 011

Simple and Highly Efficient Detection of PSD95 Using a Nanobody and Its Recombinant Heavy-Chain Antibody Derivatives.
Kilisch M, Gere-Becker M, Wüstefeld L, Bonnas C, Crauel A, Mechmershausen M, Martens H, Götzke H, Opazo F, Frey S
International journal of molecular sciences (2023) 248: . 251 011 WB; tested species: mouse

Depressed glutamate transporter 1 expression in a mouse model of Dravet syndrome.
Hameed MQ, Hui B, Lin R, MacMullin PC, Pascual-Leone A, Vermudez SAD, Rotenberg A
Annals of clinical and translational neurology (2023) 109: 1695-1699. 251 011 WB; tested species: mouse

Auxiliary α2δ1 and α2δ3 Subunits of Calcium Channels Drive Excitatory and Inhibitory Neuronal Network Development.
Bikbaev A, Ciuraszkiewicz-Wojciech A, Heck J, Klatt O, Freund R, Mitlöhner J, Enrile Lacalle S, Sun M, Repetto D, Frischknecht R, Ablinger C, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) 4025: 4824-4841. 251 011 WB; tested species: mouse

PLA2G4E, a candidate gene for resilience in Alzheimer´s disease and a new target for dementia treatment.
Pérez-González M, Mendioroz M, Badesso S, Sucunza D, Roldan M, Espelosín M, Ursua S, Luján R, Cuadrado-Tejedor M, Garcia-Osta A
Progress in neurobiology (2020) 191: 101818. 251 011 WB; tested species: mouse

TBC1D24 regulates axonal outgrowth and membrane trafficking at the growth cone in rodent and human neurons.
Aprile D, Fruscione F, Baldassari S, Fadda M, Ferrante D, Falace A, Buhler E, Sartorelli J, Represa A, Baldelli P, Benfenati F, et al.
Cell death and differentiation (2019) : . 251 011 WB; tested species: rat

Cat. No.: 251 011

Amount: 100 µg

Price: $420.00

ICC

Background

The two major cytoskeletal proteins involved in cell motility are myosin and actin. Monomeric actin is a globular protein that is expressed in all eukaryotic cells. Actin is the major subunit of microfilaments, a major component of the cytoskeleton, and of thin filaments, part of the contractile apparatus in muscle cells.
Actin is involved in many cellular processes including cell motility, maintenance of cell shape, and organelle trafficking.
Three main groups of actin have been identified. α-actins are found in muscle tissues whereas β- and γ-actins co-exist in most cell types as components of the cytoskeleton.

1 ) Bassell GJ et al., J. Neurosci. (1998)
2 ) Ng SY et al., Nucleic Acids Res. (1989)
3 ) Nudel U et al., Nucleic Acids Res. (1983)
4 ) Czosnek H et al., EMBO J. (1983)

Protocols

Standard protocol

ICC

Other products for this target

beta-Actin antibody - 251 011

References for beta-Actin - 251 011