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Home > Products > 250 204

EAAT2 antibody extracellular domain - 250 204 K.O.

EAATs are transmembrane proteins involved in the removal of extracellular glutamate
Guinea pig polyclonal antiserum
Cat. No.: 250 204
Amount: 100 µl
Price: $365.00
Cat. No. 250 204 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: 1 : 500 gallery  
Immunogen Synthetic peptide corresponding to AA 146 to 161 from mouse EAAT2 (UniProt Id: P43006)
Reactivity Reacts with: rat (P31596), mouse (P43006).
Other species not tested yet.
Specificity K.O. validated
Matching control protein/peptide 250-2P
Data sheet 250_204.pdf
Cat. No.: 250 204
Amount: 100 µl
Price: $365.00
WB
ICC
IHC
IHC-P
Background
Glutamate is the major excitatory neurotransmitter in the mammalian central nervous system. After the release of glutamate from synaptic vesicles into the synaptic cleft during neurotransmission, excitatory amino acid transporters (EAATs) remove extracellular glutamate to avoid excitotoxic levels.
Five EAATs with differential expression patterns have been described so far: EAAT 1, also referred to as GLAST and SLC1A3, has neuroprotective potential following ischemia and is expressed by reactive astrocytes and activated microglia. EAAT 2 (GLT-1, SLC1A2) is the most abundant and primarily expressed in astrocytes. EAAT 3 / SLC1A1 is expressed in neurons and has also been shown to be involved in the uptake of extracelluar cysteine. EAAT 4 shows weak expression in the forebrain and high levels in Purkinje cells of the cerebellum. EAAT 5 has only been described for humans and is primarily expressed in the retina.
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