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Kv3.1b antibody - 242 003 K.O.

Kv3.1b is a potassium channel enriched on fast spiking neurons
Rabbit polyclonal purified antibody
Cat. No.: 242 003
Amount: 50 µg
Price: $375.00
Cat. No. 242 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P: not tested yet
EM: yes

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Electron microscopy (EM) is a microscopy technique that detects the scatter of electrons through thin tissue sections. In Immuno-EM the antigen is usually revealed by colloidal gold conjugated secondary antibodies linking electron dense structures to antigen-bound primary antibodies.
Immunogen Synthetic peptide corresponding to AA 567 to 585 from mouse Kv3.1b (UniProt Id: P15388)
Reactivity Reacts with: human (P48547), rat (P25122), mouse (P15388), cow.
No signal: zebrafish.
Other species not tested yet.
Specificity K.O. validated PubMed: 35510987
Matching control protein/peptide 242-0P
Data sheet 242_003.pdf

References for Kv3.1b - 242 003

HCN channels at the cell soma ensure the rapid electrical reactivity of fast-spiking interneurons in human neocortex.
Szegedi V, Bakos E, Furdan S, Kovács BH, Varga D, Erdélyi M, Barzó P, Szücs A, Tamás G, Lamsa K
PLoS biology (2023) 212: e3002001. 242 003 IHC; tested species: human,mouse
Kv3.3 subunits control presynaptic action potential waveform and neurotransmitter release at a central excitatory synapse.
Richardson A, Ciampani V, Stancu M, Bondarenko K, Newton S, Steinert JR, Pilati N, Graham BP, Kopp-Scheinpflug C, Forsythe ID
eLife (2022) 11: . 242 003 IHC; KO verified; tested species: mouse
A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 242 003 IHC; tested species: rat
Different priming states of synaptic vesicles underlie distinct release probabilities at hippocampal excitatory synapses.
Aldahabi M, Balint F, Holderith N, Lorincz A, Reva M, Nusser Z
Neuron (2022) : . 242 003 EM; tested species: mouse
Variability in the Munc13-1 content of excitatory release sites.
Karlocai MR, Heredi J, Benedek T, Holderith N, Lorincz A, Nusser Z
eLife (2021) 10: . 242 003 EM; tested species: mouse
Cat. No.: 242 003
Amount: 50 µg
Price: $375.00
HCN channels at the cell soma ensure the rapid electrical reactivity of fast-spiking interneurons in human neocortex.
Szegedi V, Bakos E, Furdan S, Kovács BH, Varga D, Erdélyi M, Barzó P, Szücs A, Tamás G, Lamsa K
PLoS biology (2023) 212: e3002001. 242 003 IHC; tested species: human,mouse
Kv3.3 subunits control presynaptic action potential waveform and neurotransmitter release at a central excitatory synapse.
Richardson A, Ciampani V, Stancu M, Bondarenko K, Newton S, Steinert JR, Pilati N, Graham BP, Kopp-Scheinpflug C, Forsythe ID
eLife (2022) 11: . 242 003 IHC; KO verified; tested species: mouse
A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 242 003 IHC; tested species: rat
Different priming states of synaptic vesicles underlie distinct release probabilities at hippocampal excitatory synapses.
Aldahabi M, Balint F, Holderith N, Lorincz A, Reva M, Nusser Z
Neuron (2022) : . 242 003 EM; tested species: mouse
Variability in the Munc13-1 content of excitatory release sites.
Karlocai MR, Heredi J, Benedek T, Holderith N, Lorincz A, Nusser Z
eLife (2021) 10: . 242 003 EM; tested species: mouse
WB
ICC
ICC
IHC
IHC
Background
Voltage-gated potassium (Kv) channels regulate many aspects of neuronal excitability like shaping of action potentials or modulating spike patterns.
Mammalian neurons express more than 20 different Kv subunits that can be subdivided into 12 families. Heteromeric assembly of 4 subunits and differential phosphorylation of Kv channels gives rise to a huge molecular and functional diversity.
The related proteins Kv3.1 - Kv3.4 form the Shaw-type subfamily. Kv3.1b, also known as Kcnc 1, is highly enriched in neurons that fire at high frequencies such as fast spiking (FS) interneurons of the cortex and hippocampus and neurons in the globus pallidus.
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