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IBA1 antibody - 234 013

IBA1 is a marker for macrophages and microglia
Rabbit polyclonal purified antibody
Cat. No.: 234 013
Amount: 50 µg
Price: $370.00
Cat. No. 234 013 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 1000 gallery  
IHC: 1 : 1000 up to 1 : 5000      gallery  
IHC-P: 1 : 1000      gallery  
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of mouse IBA1 (UniProt Id: Q9EQW9)
Reactivity Reacts with: rat (P55009), mouse (O70200), human (P55008).
Other species not tested yet.
Specificity Specific for IBA 1. K.O.
Data sheet 234_013.pdf

References for IBA1 - 234 013

Heterogeneity of neurons reprogrammed from spinal cord astrocytes by the proneural factors Ascl1 and Neurogenin2.
Kempf J, Knelles K, Hersbach BA, Petrik D, Riedemann T, Bednarova V, Janjic A, Simon-Ebert T, Enard W, Smialowski P, Götz M, et al.
Cell reports (2021) 363: 109409. 234 013 ICC; tested species: mouse
A modified protocol for isolation of retinal microglia from the pig.
Zhang Y, Erhard AL, Plagemann T, Eter N, Heiduschka P
Experimental eye research (2021) 207: 108584. 234 013 ICC; tested species: pig
Maternal omega-3 intake differentially affects the endocannabinoid system in the progeny`s neocortex and hippocampus: Impact on synaptic markers.
Isaac AR, de Velasco PC, Fraga KYD, Tavares-do-Carmo MDG, Campos RMP, Iannotti FA, Verde R, Martins DBG, Santos TA, Ferreira BK, de Mello FG, et al.
The Journal of nutritional biochemistry (2021) 96: 108782. 234 013 IHC; tested species: rat
Classification of Microglial Morphological Phenotypes Using Machine Learning.
Leyh J, Paeschke S, Mages B, Michalski D, Nowicki M, Bechmann I, Winter K
Frontiers in cellular neuroscience (2021) 15: 701673. 234 013 IHC; tested species: mouse
Metabolic Analysis of Vitreous/Lens and Retina in Wild Type and Retinal Degeneration Mice.
Murenu E, Kostidis S, Lahiri S, Geserich AS, Imhof A, Giera M, Michalakis S
International journal of molecular sciences (2021) 225: . 234 013 IHC; tested species: mouse
Undisturbed climbing fiber pruning in the cerebellar cortex of CX3 CR1-deficient mice.
Kaiser N, Pätz C, Brachtendorf S, Eilers J, Bechmann I
Glia (2020) : . 234 013 IHC; tested species: mouse
Semaphorin 7A restricts serotonergic innervation and ensures recovery after spinal cord injury.
Loy K, Fourneau J, Meng N, Denecke C, Locatelli G, Bareyre FM
Cellular and molecular life sciences : CMLS (2020) : . 234 013 IHC; tested species: mouse
Secreted Phosphoprotein 1 Expression in Retinal Mononuclear Phagocytes Links Murine to Human Choroidal Neovascularization.
Schlecht A, Zhang P, Wolf J, Thien A, Rosmus DD, Boneva S, Schlunck G, Lange C, Wieghofer P
Frontiers in cell and developmental biology (2020) 8: 618598. 234 013 IHC; tested species: human
Cat. No.: 234 013
Amount: 50 µg
Price: $370.00
Heterogeneity of neurons reprogrammed from spinal cord astrocytes by the proneural factors Ascl1 and Neurogenin2.
Kempf J, Knelles K, Hersbach BA, Petrik D, Riedemann T, Bednarova V, Janjic A, Simon-Ebert T, Enard W, Smialowski P, Götz M, et al.
Cell reports (2021) 363: 109409. 234 013 ICC; tested species: mouse
A modified protocol for isolation of retinal microglia from the pig.
Zhang Y, Erhard AL, Plagemann T, Eter N, Heiduschka P
Experimental eye research (2021) 207: 108584. 234 013 ICC; tested species: pig
Maternal omega-3 intake differentially affects the endocannabinoid system in the progeny`s neocortex and hippocampus: Impact on synaptic markers.
Isaac AR, de Velasco PC, Fraga KYD, Tavares-do-Carmo MDG, Campos RMP, Iannotti FA, Verde R, Martins DBG, Santos TA, Ferreira BK, de Mello FG, et al.
The Journal of nutritional biochemistry (2021) 96: 108782. 234 013 IHC; tested species: rat
Classification of Microglial Morphological Phenotypes Using Machine Learning.
Leyh J, Paeschke S, Mages B, Michalski D, Nowicki M, Bechmann I, Winter K
Frontiers in cellular neuroscience (2021) 15: 701673. 234 013 IHC; tested species: mouse
Metabolic Analysis of Vitreous/Lens and Retina in Wild Type and Retinal Degeneration Mice.
Murenu E, Kostidis S, Lahiri S, Geserich AS, Imhof A, Giera M, Michalakis S
International journal of molecular sciences (2021) 225: . 234 013 IHC; tested species: mouse
Undisturbed climbing fiber pruning in the cerebellar cortex of CX3 CR1-deficient mice.
Kaiser N, Pätz C, Brachtendorf S, Eilers J, Bechmann I
Glia (2020) : . 234 013 IHC; tested species: mouse
Semaphorin 7A restricts serotonergic innervation and ensures recovery after spinal cord injury.
Loy K, Fourneau J, Meng N, Denecke C, Locatelli G, Bareyre FM
Cellular and molecular life sciences : CMLS (2020) : . 234 013 IHC; tested species: mouse
Secreted Phosphoprotein 1 Expression in Retinal Mononuclear Phagocytes Links Murine to Human Choroidal Neovascularization.
Schlecht A, Zhang P, Wolf J, Thien A, Rosmus DD, Boneva S, Schlunck G, Lange C, Wieghofer P
Frontiers in cell and developmental biology (2020) 8: 618598. 234 013 IHC; tested species: human
Background

Ionized calcium-binding adaptor molecule 1 (IBA1) or allograft inflammatory factor1 (AIF-1) is an EF hand calcium binding protein which is expressed by cells of the monocyte/macrophage lineage and by germ cells in the testis (1). In mice, IBA1/AIF-1 can be regarded a “pan-macrophage marker” because, except for alveolar macrophages, all subpopulations of macrophages express IBA1/AIF-1 (1). In human gliomas IBA1 defines a distinct subset of tumor-associated activated macrophages/microglial cells (2).
Microglia represent the resident macrophages in the nervous system and are the smallest of the glial cells with cell bodies of only 2-5 µm in diameter. In the CNS IBA1 upregulation is associated with neuroinflammatory response (3).