Tailor-made Antibodies
and Tools for Life Science

IBA1 antibody - 234 009

IBA1 is a marker for macrophages and microglia
Chicken monoclonal recombinant IgY
Cat. No.: 234 009
Amount: 50 µg
Price: $430.00
Cat. No. 234 009 50 µg purified recombinant IgY, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 2000 (AP-staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: 1 : 200 up to 1 : 1000 gallery  
ExM: 1 : 100
Clone Ch311H9
Subtype IgY (λ light chain)
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of rat IBA1 (UniProt Id: P55009)
Reactivity Reacts with: rat (P55009), mouse (Q9EQW9), human (P55008), ape.
Other species not tested yet.
Matching control protein/peptide 234-0P
Remarks

This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 311H9. The constant regions of the heavy and light chains have been replaced by chicken specific sequences. Therefore, the antibody can be used with standard anti-chicken secondary reagents. The antibody has been expressed in mammalian cells.

Data sheet 234_009.pdf

References for IBA1 - 234 009

Primary cortical cell tri-culture to study effects of amyloid-β on microglia function and neuroinflammatory response.
Kim H, Le B, Goshi N, Zhu K, Grodzki AC, Lein PJ, Zhao M, Seker E
Journal of Alzheimer's disease : JAD (2024) : 13872877241291142. 234 009 ICC; tested species: rat
Rapid phagosome isolation enables unbiased multiomic analysis of human microglial phagosomes.
Wogram E, Sümpelmann F, Dong W, Rawat E, Fernández Maestre I, Fu D, Braswell B, Khalil A, Buescher JM, Mittler G, Borner GHH, et al.
Immunity (2024) 579: 2216-2231.e11. 234 009 ICC; tested species: human
Cat. No.: 234 009
Amount: 50 µg
Price: $430.00