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- will be discontinued soon

Polyclonal antibodies are a finite resource. We have now started to sell the last lots of our well received rabbit polyclonal UCHL1 antibody 233 002. It will be replaced by our new rabbit polyclonal affinity purified UCHL1 antibody 233 003.

UCHL1 antibody - 233 002

UCHL1 or PGP9.5 is a neuron specific member of the ubiquitin proteasome system

Rabbit polyclonal antiserum

Cat. No.: 233 002

Amount: 200 µl

Price: $360.00

Cat. No. 233 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 2 to 223 from mouse UCHL1 (UniProt Id: Q9R0P9)
Reactivity	Reacts with: rat (Q00981), mouse (Q9R0P9). No signal: zebrafish. Other species not tested yet.
Data sheet	233_002.pdf

Cat. No.: 233 002

Amount: 200 µl

Price: $360.00

Background

The Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL 1) enzyme is a member of a protein family involved in the hydrolyzation of C-terminal adducts of polymeric ubiquitin to generate monomeric ubiquitin. It is abundantly expressed in neurons throughout the brain and belongs to the ubiquitin-proteasome system. This pathway is responsible for the degradation of misfolded and damaged proteins and has been postulated to play an important role in Parkinson's disease.

1 ) Ragland M et al., Am. J. Epidemiol. (2009)
2 ) Xu EH et al., J Clin Neurosci (2009)
3 ) Zhang ZJ et al., Neurosci. Lett. (2008)
4 ) Hutter CM et al., Eur. J. Neurol. (2008)
5 ) Lombardino AJ et al., Proc. Natl. Acad. Sci. U.S.A. (2005)
6 ) Otsuki T et al., Br. J. Haematol. (2004)
7 ) Satoh JI et al., Neuropathol. Appl. Neurobiol. (2001)
8 ) Trowern AR et al., Neurosci. Lett. (1996)

Protocols

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