Synaptic Systems

Cat. No.: 232 011

Amount: 100 µg

Price: $415.00

Cat. No. 232 011	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: not tested yet gallery IHC: not tested yet gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	158.2
Subtype	IgG2a (κ light chain)
Immunogen	Recombinant protein corresponding to AA 119 to 188 from mouse VAMP7 (UniProt Id: P70280)
Reactivity	Reacts with: rat (Q9JHW5), mouse (P70280), human (P51809), chicken. No signal: zebrafish. Other species not tested yet.
Specificity	K.O. validated PubMed: 30271964
Data sheet	232_011.pdf

References for VAMP7 - 232 011

Comparative study of commercially available and homemade anti-VAMP7 antibodies using CRISPR/Cas9-depleted HeLa cells and VAMP7 knockout mice.
Verraes A, Cholley B, Galli T, Nola S
F1000Research (2018) 7: 1649. 232 011 WB; KO verified; tested species: mouse

Vesicle-associated membrane protein 7-mediated eosinophil degranulation promotes allergic airway inflammation in mice.
Willetts L, Felix LC, Jacobsen EA, Puttagunta L, Condjella RM, Zellner KR, Ochkur SI, Kim JD, Luo H, Lee NA, Lee JJ, et al.
Communications biology (2018) 1: 83. 232 011 WB, ICC; KO verified; tested species: human

Cyclic Nucleotide Control of Microtubule Dynamics for Axon Guidance.
Akiyama H, Fukuda T, Tojima T, Nikolaev VO, Kamiguchi H
The Journal of neuroscience : the official journal of the Society for Neuroscience (2016) 3620: 5636-49. 232 011 WB; KD verified; tested species: chicken

VAMP-7 links granule exocytosis to actin reorganization during platelet activation.
Koseoglu S, Peters CG, Fitch-Tewfik JL, Aisiku O, Danglot L, Galli T, Flaumenhaft R
Blood (2015) 1265: 651-60. 232 011 WB

Tetanus neurotoxin-insensitive vesicle-associated membrane protein localizes to a presynaptic membrane compartment in selected terminal subsets of the rat brain.
Muzerelle A, Alberts P, Martinez-Arca S, Jeannequin O, Lafaye P, Mazié JC, Galli T, Gaspar P
Neuroscience (2003) 1221: 59-75. 232 011 WB, IHC

Lysosomal exocytosis releases pathogenic α-synuclein species from neurons in synucleinopathy models.
Xie YX, Naseri NN, Fels J, Kharel P, Na Y, Lane D, Burré J, Sharma M
Nature communications (2022) 131: 4918. 232 011 IP; tested species: mouse

An actin cytoskeletal barrier inhibits lytic granule release from natural killer cells in patients with Chediak-Higashi syndrome.
Gil-Krzewska A, Saeed MB, Oszmiana A, Fischer ER, Lagrue K, Gahl WA, Introne WJ, Coligan JE, Davis DM, Krzewski K
The Journal of allergy and clinical immunology (2017) : . 232 011 ICC; tested species: human

Human autoantibodies to amphiphysin induce defective presynaptic vesicle dynamics and composition.
Werner C, Pauli M, Doose S, Weishaupt A, Haselmann H, Grünewald B, Sauer M, Heckmann M, Toyka KV, Asan E, Sommer C, et al.
Brain : a journal of neurology (2016) 139Pt 2: 365-79. 232 011 ICC; tested species: rat

Cat. No.: 232 011

Amount: 100 µg

Price: $415.00

ICC

IHC

Background

VAMP 7, also referred to as Ti-VAMP and SybL 1, is a member of the SNARE family of proteins and a relative of synaptobrevin. It is involved in membrane fusion events that mediate neurite outgrowth in developing neurons, in endosome to lysosome transport and in other cellular trafficking mechanisms. VAMP 7 is ubiquitously expressed in different tissues.
It is a member of the syntaxin 4-SNAP 23-VAMP 7- and the syntaxin 7-syntaxin 8-Vti1b-VAMP 7-SNARE complex.

1 ) Siddiqi SA et al., J. Cell. Sci. (2006)
2 ) Rao SK et al., J. Biol. Chem. (2004)
3 ) Martinez-Arca S et al., Proc. Natl. Acad. Sci. U.S.A. (2003)
4 ) Coco S et al., J. Neurosci. (1999)
5 ) Advani RJ et al., J. Cell Biol. (1999)

Protocols

Standard protocol

Other products for this target

VAMP7 antibody - 232 011 K.O.

References for VAMP7 - 232 011