Synaptic Systems

Cat. No.: 232 003

Amount: 50 µg

Price: $375.00

Cat. No. 232 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not recommended ICC: not recommended IHC: 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 10 to 177 from mouse VAMP7 (UniProt Id: P70280)
Reactivity	Reacts with: rat (Q9JHW5), mouse (P70280), human (P51809). No signal: zebrafish. Other species not tested yet.
Specificity	K.O. validated PubMed: 30815249
Matching control protein/peptide	232-0P
Data sheet	232_003.pdf

References for VAMP7 - 232 003

Comparative study of commercially available and homemade anti-VAMP7 antibodies using CRISPR/Cas9-depleted HeLa cells and VAMP7 knockout mice.
Verraes A, Cholley B, Galli T, Nola S
F1000Research (2018) 7: 1649. 232 003 WB; KO verified; tested species: human

Lysosomal exocytosis releases pathogenic α-synuclein species from neurons in synucleinopathy models.
Xie YX, Naseri NN, Fels J, Kharel P, Na Y, Lane D, Burré J, Sharma M
Nature communications (2022) 131: 4918. 232 003 WB; tested species: mouse

Characterisation of GLUT4 trafficking in HeLa cells: comparable kinetics and orthologous trafficking mechanisms to 3T3-L1 adipocytes.
Morris S, Geoghegan ND, Sadler JBA, Koester AM, Black HL, Laub M, Miller L, Heffernan L, Simpson JC, Mastick CC, Cooper J, et al.
PeerJ (2020) 8: e8751. 232 003 WB; tested species: mouse

VAMP4 maintains a Ca2+-sensitive pool of spontaneously recycling synaptic vesicles.
Lin PY, Chanaday NL, Horvath PM, Ramirez DMO, Monteggia LM, Kavalali ET
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 232 003 WB; tested species: rat

A trap mutant reveals the physiological client spectrum of TRC40.
Coy-Vergara J, Rivera-Monroy J, Urlaub H, Lenz C, Schwappach B
Journal of cell science (2019) 13213: . 232 003 WB; tested species: human

Cardiac SNARE Expression in Health and Disease.
Bowman PRT, Smith GL, Gould GW
Frontiers in endocrinology (2019) 10: 881. 232 003 WB; tested species: mouse

Selective molecular impairment of spontaneous neurotransmission modulates synaptic efficacy.
Crawford DC, Ramirez DM, Trauterman B, Monteggia LM, Kavalali ET
Nature communications (2017) 8: 14436. 232 003 WB; KD verified

Differential Expression of Munc13-2 Produces Unique Synaptic Phenotypes in the Basolateral Amygdala of C57BL/6J and DBA/2J Mice.
Gioia DA, Alexander NJ, McCool BA
The Journal of neuroscience : the official journal of the Society for Neuroscience (2016) 3643: 10964-10977. 232 003 WB

Characterization of VAMP isoforms in 3T3-L1 adipocytes: implications for GLUT4 trafficking.
Sadler JB, Bryant NJ, Gould GW
Molecular biology of the cell (2015) 263: 530-6. 232 003 WB

Syntaxin 11 binds Vti1b and regulates late endosome to lysosome fusion in macrophages.
Offenhäuser C, Lei N, Roy S, Collins BM, Stow JL, Murray RZ
Traffic (Copenhagen, Denmark) (2011) 126: 762-73. 232 003 WB

Single synapse glutamate imaging reveals multiple levels of release mode regulation in mammalian synapses.
Farsi Z, Walde M, Klementowicz AE, Paraskevopoulou F, Woehler A
iScience (2021) 241: 101909. 232 003 ICC; tested species: rat

Proteomic analysis reveals the composition of glutamatergic organelles of auditory inner hair cell.
Cepeda AP, Ninov M, Neef J, Parfentev I, Kusch K, Reisinger E, Jahn R, Moser T, Urlaub H
Molecular & cellular proteomics : MCP (2023) : 100704. 232 003 IHC; tested species: mouse

Cat. No.: 232 003

Amount: 50 µg

Price: $375.00

Cardiac SNARE Expression in Health and Disease.
Bowman PRT, Smith GL, Gould GW
Frontiers in endocrinology (2019) 10: 881. 232 003 WB; tested species: mouse

Characterization of VAMP isoforms in 3T3-L1 adipocytes: implications for GLUT4 trafficking.
Sadler JB, Bryant NJ, Gould GW
Molecular biology of the cell (2015) 263: 530-6. 232 003 WB

IHC

Background

VAMP 7, also referred to as Ti-VAMP and SybL 1, is a member of the SNARE family of proteins and a relative of synaptobrevin. It is involved in membrane fusion events that mediate neurite outgrowth in developing neurons, in endosome to lysosome transport and in other cellular trafficking mechanisms. VAMP 7 is ubiquitously expressed in different tissues.
It is a member of the syntaxin 4-SNAP 23-VAMP 7- and the syntaxin 7-syntaxin 8-Vti1b-VAMP 7-SNARE complex.

1 ) Siddiqi SA et al., J. Cell. Sci. (2006)
2 ) Rao SK et al., J. Biol. Chem. (2004)
3 ) Martinez-Arca S et al., Proc. Natl. Acad. Sci. U.S.A. (2003)
4 ) Coco S et al., J. Neurosci. (1999)
5 ) Advani RJ et al., J. Cell Biol. (1999)

Protocols

IHC

OTHER

Preabsorbtion

Other products for this target

VAMP7 antibody - 232 003 K.O.

References for VAMP7 - 232 003