Synaptic Systems

Cat. No.: 231 103

Amount: 50 µg

Price: $375.00

Microglia monitor and protect neuronal function through specialized somatic purinergic junctions.
Cserép C, Pósfai B, Lénárt N, Fekete R, László ZI, Lele Z, Orsolits B, Molnár G, Heindl S, Schwarcz AD, Ujvári K, et al.
Science (New York, N.Y.) (2020) 3676477: 528-537. 231 103 IHC; tested species: mouse

WB

Background

Voltage-gated potassium (Kv) channels regulate many aspects of neuronal excitability like shaping of action potentials or modulating of spike patterns.
Mammalian neurons express more than 20 different Kv subunits that can be subdivided into 12 families. Heteromeric assembly of four subunits and differential phosphorylation of Kv channels give rise to a huge molecular and functional diversity.
The Kv 2 subfamily comprising Kv 2.1 (DRK 1, Kcnb 1) and Kv 2.2 (CDRK, Kcnb 2) is a unique exception since they do not form heterodimers. Kv 2.1 is found in large clusters on the soma and proximal dendrites of pyramidal neurons.

1 ) Kihira Y et al., J. Biol. Chem. (2010)
2 ) Hermanstyne TO et al., J. Comp. Neurol. (2010)
3 ) Wray D et al., Eur. Biophys. J. (2009)
4 ) Mulholland PJ et al., J. Neurosci. (2008)
5 ) Wolf-Goldberg T et al., Mol. Pharmacol. (2006)
6 ) Kobrinsky E et al., J. Biol. Chem. (2006)
7 ) O'Connell KM et al., J. Neurosci. (2006)
8 ) Park KS et al., Science (2006)
9 ) Lim ST et al., Neuron (2000)
10 ) Murakoshi H et al., Mol. Pharmacol. (1997)

Protocols

WB

IP

Standard protocol

OTHER

Preabsorbtion

Other products for this target

Cat. No. 231 103	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 859 to 873 from rat Kv2.2 (UniProt Id: Q63099)
Reactivity	Reacts with: rat (Q63099), mouse (A6H8H5). No signal: zebrafish. Other species not tested yet.
Matching control protein/peptide	231-1P
Data sheet	231_103.pdf

Kv2.2 antibody - 231 103

References for Kv2.2 - 231 103