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c-Fos antibody - 226 017

c-Fos is a member of the transcription factor complex AP1
Rat monoclonal purified IgG
Cat. No.: 226 017
Amount: 100 µg
Price: $425.00
Cat. No. 226 017 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 1000 up to 1 : 5000 (see remarks) gallery  
IHC-P: 1 : 100 up to 1 : 200 gallery  
iDISCO: 1 : 500
FACS: yes gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

iDISCO is a fast and cost-effective sample preparation method for clearing, immunolabeling and volume imaging of large biological samples. The clearing is carried out by benzyl-ether treatment. For additional experimental details, please refer to the „Remarks“ section or visit https://idisco.info.

Fluorescence-activated cell sorting (FACS); usually cells are fluorescently labeled with antibodies against cell-surface antigens followed by cell-sorter analysis.
Clone 108B5H5
Subtype IgG2a (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the amino terminus of rat c-Fos (UniProt Id: P12841)
Reactivity Reacts with: mouse (P01101), rat (P12841), human (P01100).
Other species not tested yet.
Matching control protein/peptide 226-0P
Remarks

IHC: For best results tissue sections should be stored at -20°C in cryoprotectant solution. Prolonged storage at 4°C leads to a substantial loss of signal.
Data sheet 226_017.pdf

References for c-Fos - 226 017

Transcriptional dynamics of murine motor neuron maturation in vivo and in vitro.
Patel T, Hammelman J, Aziz S, Jang S, Closser M, Michaels TL, Blum JA, Gifford DK, Wichterle H
Nature communications (2022) 131: 5427. 226 017 IHC, ICC; tested species: mouse
Learning-associated astrocyte ensembles regulate memory recall.
Williamson MR, Kwon W, Woo J, Ko Y, Maleki E, Yu K, Murali S, Sardar D, Deneen B
Nature (2024) : . 226 017 IHC; tested species: mouse
A multiple Arc (mArc) tagging system to uncover the organizational principles of multiple memories.
Stackmann M, Yelhekar TD, Meng M, Sun X, Nthumba J, Bulthuis NE, Vaughan N, Zhu E, Lin Y, Denny CA
bioRxiv : the preprint server for biology (2024) : . 226 017 IHC; tested species: mouse
Melatonin targets the paraventricular thalamus to promote non-rapid eye movement sleep in C3H/HeJ mice.
Wang Y, Song Z, Han Q, Luo F, Jiang C, Zhang Z, Wang N, Zou N, Liu G, Long M, Liu H, et al.
Current biology : CB (2024) 3416: 3792-3803.e5. 226 017 IHC; tested species: mouse
Activation of Gs Signaling in Cortical Astrocytes Does Not Influence Formation of a Persistent Contextual Memory Engram.
Mak A, Abramian A, Driessens SLW, Boers-Escuder C, van der Loo RJ, Smit AB, van den Oever MC, Verheijen MHG
eNeuro (2024) 116: . 226 017 IHC; tested species: mouse
Rapid, biochemical tagging of cellular activity history in vivo.
Zhang R, Anguiano M, Aarrestad IK, Lin S, Chandra J, Vadde SS, Olson DE, Kim CK
Nature methods (2024) 219: 1725-1735. 226 017 IHC; tested species: mouse
Fasting-induced activity changes in MC3R neurons of the paraventricular nucleus of the thalamus.
Chesters RA, Zhu J, Coull BM, Baidoe-Ansah D, Baumer L, Palm L, Klinghammer N, Chen S, Hahm A, Yagoub S, Cantacorps L, et al.
Life science alliance (2024) 710: . 226 017 IHC; tested species: mouse
Rapid reorganization of serotonin projections and antidepressant response to 5-HT1A-biased agonist NLX-101 in fluoxetine-resistant cF1ko mice.
Vahid-Ansari F, Newman-Tancredi A, Fuentes-Alvarenga AF, Daigle M, Albert PR
Neuropharmacology (2024) 261: 110132. 226 017 IHC; tested species: mouse
Divergent recruitment of developmentally defined neuronal ensembles supports memory dynamics.
Kveim VA, Salm L, Ulmer T, Lahr M, Kandler S, Imhof F, Donato F
Science (New York, N.Y.) (2024) 3856710: eadk0997. 226 017 IHC; tested species: mouse
Neural correlates of learning and memory are altered by early-life stress.
Sanguino-Gómez J, Huijgens S, den Hartog M, Schenk IJM, Kluck W, Versluis TD, Krugers HJ
Neurobiology of learning and memory (2024) 213: 107952. 226 017 IHC; tested species: mouse
Optogenetic activation of the lateral habenulaD1R-ventral tegmental area circuit induces depression-like behavior in mice.
Chen X, Liu X, Luan S, Wang X, Zhang Y, Hao Y, Zhang Q, Zhang J, Zhao H
European archives of psychiatry and clinical neuroscience (2024) 2744: 867-878. 226 017 IHC; tested species: mouse
Astrocytes in preoptic area regulate acute nociception-induced hypothermia through adenosine receptors.
Jia J, Chen T, Chen C, Si T, Gao C, Fang Y, Sun J, Wang J, Zhang Z
CNS neuroscience & therapeutics (2024) 305: e14726. 226 017 IHC; tested species: mouse
Hippocampus-to-amygdala pathway drives the separation of remote memories of related events.
Concina G, Milano L, Renna A, Manassero E, Stabile F, Sacchetti B
Cell reports (2024) 435: 114151. 226 017 IHC; tested species: rat
Stimulating intestinal GIP release reduces food intake and body weight in mice.
Lewis JE, Nuzzaci D, James-Okoro PP, Montaner M, O'Flaherty Rottenberger E, Darwish T, Hayashi M, Liberles SD, Hornigold D, Naylor J, Baker D, et al.
Molecular metabolism (2024) : 101945. 226 017 IHC; tested species: mouse
Prelimbic area to lateral hypothalamus circuit drives social aggression.
Bai F, Huang L, Deng J, Long Z, Hao X, Chen P, Wu G, Wen H, Deng Q, Bao X, Huang J, et al.
iScience (2023) 2610: 107718. 226 017 IHC; tested species: mouse
Whole-brain analysis of CO2 chemosensitive regions and identification of the retrotrapezoid and medullary raphé nuclei in the common marmoset (Callithrix jacchus).
Turk AZ, Millwater M, SheikhBahaei S
bioRxiv : the preprint server for biology (2023) : . 226 017 IHC; tested species: marmoset
Bidirectional Regulation of Motor Circuits Using Magnetogenetic Gene Therapy.
Unda SR, Marongiu R, Pomeranz LE, Dyke JP, Fung EK, Grosenick L, Zirkel R, Antoniazzi AM, Norman S, Liston CM, Schaffer C, et al.
bioRxiv : the preprint server for biology (2023) : . 226 017 IHC; tested species: mouse
Chronic Pb Exposure Induces Anxiety and Depression-like Behaviors in Mice via Excitatory Neuronal Hyperexcitability in Ventral Hippocampal Dentate Gyrus.
Zhou R, Huang C, Bi N, Li L, Li C, Gu X, Song Y, Wang HL
Environmental science & technology (2023) 5733: 12222-12233. 226 017 IHC; tested species: mouse
Cry1Δ11 mutation induces ADHD-like symptoms through hyperactive dopamine D1 receptor signaling.
Liu D, Xie Z, Gu P, Li X, Zhang Y, Wang X, Chen Z, Deng S, Shu Y, Li JD
JCI insight (2023) 816: . 226 017 IHC; tested species: mouse
Adrenergic signalling to astrocytes in anterior cingulate cortex contributes to pain-related aversive memory in rats.
Iqbal Z, Lei Z, Ramkrishnan AS, Liu S, Hasan M, Akter M, Lam YY, Li Y
Communications biology (2023) 61: 10. 226 017 IHC; tested species: rat
Transcriptional dynamics of murine motor neuron maturation in vivo and in vitro.
Patel T, Hammelman J, Aziz S, Jang S, Closser M, Michaels TL, Blum JA, Gifford DK, Wichterle H
Nature communications (2022) 131: 5427. 226 017 IHC, ICC; tested species: mouse
Thalamo-hippocampal pathway regulates incidental memory capacity in mice.
Torromino G, Loffredo V, Cavezza D, Sonsini G, Esposito F, Crevenna AH, Gioffrè M, De Risi M, Treves A, Griguoli M, De Leonibus E, et al.
Nature communications (2022) 131: 4194. 226 017 IHC; tested species: mouse
ΔFosB accumulation in hippocampal granule cells drives cFos pattern separation during spatial learning.
Lamothe-Molina PJ, Franzelin A, Beck L, Li D, Auksutat L, Fieblinger T, Laprell L, Alhbeck J, Gee CE, Kneussel M, Engel AK, et al.
Nature communications (2022) 131: 6376. 226 017 IHC; tested species: mouse
Reprogramming reactive glia into interneurons reduces chronic seizure activity in a mouse model of mesial temporal lobe epilepsy.
Lentini C, d'Orange M, Marichal N, Trottmann MM, Vignoles R, Foucault L, Verrier C, Massera C, Raineteau O, Conzelmann KK, Rival-Gervier S, et al.
Cell stem cell (2021) : . 226 017 IHC; tested species: mouse
Non-invasive optogenetics with ultrasound-mediated gene delivery and red-light excitation.
Pouliopoulos AN, Murillo MF, Noel RL, Batts AJ, Ji R, Kwon N, Yu H, Tong CK, Gelinas JN, Araghy DK, Hussaini SA, et al.
Brain stimulation () 154: 927-941. 226 017 IHC; tested species: mouse
Cat. No.: 226 017
Amount: 100 µg
Price: $425.00
Transcriptional dynamics of murine motor neuron maturation in vivo and in vitro.
Patel T, Hammelman J, Aziz S, Jang S, Closser M, Michaels TL, Blum JA, Gifford DK, Wichterle H
Nature communications (2022) 131: 5427. 226 017 IHC, ICC; tested species: mouse
Learning-associated astrocyte ensembles regulate memory recall.
Williamson MR, Kwon W, Woo J, Ko Y, Maleki E, Yu K, Murali S, Sardar D, Deneen B
Nature (2024) : . 226 017 IHC; tested species: mouse
A multiple Arc (mArc) tagging system to uncover the organizational principles of multiple memories.
Stackmann M, Yelhekar TD, Meng M, Sun X, Nthumba J, Bulthuis NE, Vaughan N, Zhu E, Lin Y, Denny CA
bioRxiv : the preprint server for biology (2024) : . 226 017 IHC; tested species: mouse
Melatonin targets the paraventricular thalamus to promote non-rapid eye movement sleep in C3H/HeJ mice.
Wang Y, Song Z, Han Q, Luo F, Jiang C, Zhang Z, Wang N, Zou N, Liu G, Long M, Liu H, et al.
Current biology : CB (2024) 3416: 3792-3803.e5. 226 017 IHC; tested species: mouse
Activation of Gs Signaling in Cortical Astrocytes Does Not Influence Formation of a Persistent Contextual Memory Engram.
Mak A, Abramian A, Driessens SLW, Boers-Escuder C, van der Loo RJ, Smit AB, van den Oever MC, Verheijen MHG
eNeuro (2024) 116: . 226 017 IHC; tested species: mouse
Rapid, biochemical tagging of cellular activity history in vivo.
Zhang R, Anguiano M, Aarrestad IK, Lin S, Chandra J, Vadde SS, Olson DE, Kim CK
Nature methods (2024) 219: 1725-1735. 226 017 IHC; tested species: mouse
Fasting-induced activity changes in MC3R neurons of the paraventricular nucleus of the thalamus.
Chesters RA, Zhu J, Coull BM, Baidoe-Ansah D, Baumer L, Palm L, Klinghammer N, Chen S, Hahm A, Yagoub S, Cantacorps L, et al.
Life science alliance (2024) 710: . 226 017 IHC; tested species: mouse
Rapid reorganization of serotonin projections and antidepressant response to 5-HT1A-biased agonist NLX-101 in fluoxetine-resistant cF1ko mice.
Vahid-Ansari F, Newman-Tancredi A, Fuentes-Alvarenga AF, Daigle M, Albert PR
Neuropharmacology (2024) 261: 110132. 226 017 IHC; tested species: mouse
Divergent recruitment of developmentally defined neuronal ensembles supports memory dynamics.
Kveim VA, Salm L, Ulmer T, Lahr M, Kandler S, Imhof F, Donato F
Science (New York, N.Y.) (2024) 3856710: eadk0997. 226 017 IHC; tested species: mouse
Neural correlates of learning and memory are altered by early-life stress.
Sanguino-Gómez J, Huijgens S, den Hartog M, Schenk IJM, Kluck W, Versluis TD, Krugers HJ
Neurobiology of learning and memory (2024) 213: 107952. 226 017 IHC; tested species: mouse
Optogenetic activation of the lateral habenulaD1R-ventral tegmental area circuit induces depression-like behavior in mice.
Chen X, Liu X, Luan S, Wang X, Zhang Y, Hao Y, Zhang Q, Zhang J, Zhao H
European archives of psychiatry and clinical neuroscience (2024) 2744: 867-878. 226 017 IHC; tested species: mouse
Astrocytes in preoptic area regulate acute nociception-induced hypothermia through adenosine receptors.
Jia J, Chen T, Chen C, Si T, Gao C, Fang Y, Sun J, Wang J, Zhang Z
CNS neuroscience & therapeutics (2024) 305: e14726. 226 017 IHC; tested species: mouse
Hippocampus-to-amygdala pathway drives the separation of remote memories of related events.
Concina G, Milano L, Renna A, Manassero E, Stabile F, Sacchetti B
Cell reports (2024) 435: 114151. 226 017 IHC; tested species: rat
Stimulating intestinal GIP release reduces food intake and body weight in mice.
Lewis JE, Nuzzaci D, James-Okoro PP, Montaner M, O'Flaherty Rottenberger E, Darwish T, Hayashi M, Liberles SD, Hornigold D, Naylor J, Baker D, et al.
Molecular metabolism (2024) : 101945. 226 017 IHC; tested species: mouse
Prelimbic area to lateral hypothalamus circuit drives social aggression.
Bai F, Huang L, Deng J, Long Z, Hao X, Chen P, Wu G, Wen H, Deng Q, Bao X, Huang J, et al.
iScience (2023) 2610: 107718. 226 017 IHC; tested species: mouse
Whole-brain analysis of CO2 chemosensitive regions and identification of the retrotrapezoid and medullary raphé nuclei in the common marmoset (Callithrix jacchus).
Turk AZ, Millwater M, SheikhBahaei S
bioRxiv : the preprint server for biology (2023) : . 226 017 IHC; tested species: marmoset
Bidirectional Regulation of Motor Circuits Using Magnetogenetic Gene Therapy.
Unda SR, Marongiu R, Pomeranz LE, Dyke JP, Fung EK, Grosenick L, Zirkel R, Antoniazzi AM, Norman S, Liston CM, Schaffer C, et al.
bioRxiv : the preprint server for biology (2023) : . 226 017 IHC; tested species: mouse
Chronic Pb Exposure Induces Anxiety and Depression-like Behaviors in Mice via Excitatory Neuronal Hyperexcitability in Ventral Hippocampal Dentate Gyrus.
Zhou R, Huang C, Bi N, Li L, Li C, Gu X, Song Y, Wang HL
Environmental science & technology (2023) 5733: 12222-12233. 226 017 IHC; tested species: mouse
Cry1Δ11 mutation induces ADHD-like symptoms through hyperactive dopamine D1 receptor signaling.
Liu D, Xie Z, Gu P, Li X, Zhang Y, Wang X, Chen Z, Deng S, Shu Y, Li JD
JCI insight (2023) 816: . 226 017 IHC; tested species: mouse
Adrenergic signalling to astrocytes in anterior cingulate cortex contributes to pain-related aversive memory in rats.
Iqbal Z, Lei Z, Ramkrishnan AS, Liu S, Hasan M, Akter M, Lam YY, Li Y
Communications biology (2023) 61: 10. 226 017 IHC; tested species: rat
Transcriptional dynamics of murine motor neuron maturation in vivo and in vitro.
Patel T, Hammelman J, Aziz S, Jang S, Closser M, Michaels TL, Blum JA, Gifford DK, Wichterle H
Nature communications (2022) 131: 5427. 226 017 IHC, ICC; tested species: mouse
Thalamo-hippocampal pathway regulates incidental memory capacity in mice.
Torromino G, Loffredo V, Cavezza D, Sonsini G, Esposito F, Crevenna AH, Gioffrè M, De Risi M, Treves A, Griguoli M, De Leonibus E, et al.
Nature communications (2022) 131: 4194. 226 017 IHC; tested species: mouse
ΔFosB accumulation in hippocampal granule cells drives cFos pattern separation during spatial learning.
Lamothe-Molina PJ, Franzelin A, Beck L, Li D, Auksutat L, Fieblinger T, Laprell L, Alhbeck J, Gee CE, Kneussel M, Engel AK, et al.
Nature communications (2022) 131: 6376. 226 017 IHC; tested species: mouse
Reprogramming reactive glia into interneurons reduces chronic seizure activity in a mouse model of mesial temporal lobe epilepsy.
Lentini C, d'Orange M, Marichal N, Trottmann MM, Vignoles R, Foucault L, Verrier C, Massera C, Raineteau O, Conzelmann KK, Rival-Gervier S, et al.
Cell stem cell (2021) : . 226 017 IHC; tested species: mouse
Non-invasive optogenetics with ultrasound-mediated gene delivery and red-light excitation.
Pouliopoulos AN, Murillo MF, Noel RL, Batts AJ, Ji R, Kwon N, Yu H, Tong CK, Gelinas JN, Araghy DK, Hussaini SA, et al.
Brain stimulation () 154: 927-941. 226 017 IHC; tested species: mouse
WB
ICC
IHC
IHC
IHC-P
IHC-P
IHC-P
IHC-P
FACS
FACS
Background

The Fos gene family consists of 4 members: c-Fos, FosB, FosL1, and FosL2, also called Fos related antigen 1 and 2 (FRA1 and 2). These leucine zipper proteins can dimerize with proteins of the Jun family leading to the formation of the transcription factor complex AP1 (1).
The expression of Fos proteins is rapidly and transiently induced by different extracellular stimuli such as growth factors, cytokines, neurotransmitters, polypeptide hormones and stress (2).
In addition Fos proteins can be phosphorylated by ERK kinases modulating transcriptional activity, protein stability and localization (3). c-Fos is the homologue to the Finkel-Biskis-Jinkins (FBJ) murine osteosarcoma virus oncogene (4).
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