c-Fos antibody - 226 009

c-Fos is a member of the transcription factor complex AP1

Chicken monoclonal recombinant IgY

Cat. No.: 226 009

Amount: 50 µg

Price: $415.00

Cat. No. 226 009	50 µg purified recombinant IgY, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 2000 (AP-staining) gallery ICC: yes gallery IHC: 1 : 1000 up to 1 : 5000 (see remarks) gallery IHC-P: 1 : 500 up to 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Ch108B5
Subtype	IgY (λ light chain)
Immunogen	Synthetic peptide corresponding to residues near the amino terminus of rat c-Fos (UniProt Id: P12841)
Reactivity	Reacts with: mouse (P01101), rat (P12841), human (P01100). Other species not tested yet.
Matching control protein/peptide	226-0P
Remarks	This antibody is a chimeric antibody based on the monoclonal rat antibody clone 108B5. The constant regions of the heavy and light chains have been replaced by chicken specific sequences. Therefore, the antibody can be used with standard anti-chicken secondary reagents. The antibody has been expressed in mammalian cells. IHC: For best results, tissue sections should be stored at -20°C in cryoprotectant solution. Prolonged storage at 4°C leads to a substantial loss of signal.
Data sheet	226_009.pdf

Cat. No.: 226 009

Amount: 50 µg

Price: $415.00

ICC

IHC

IHC-P

Background

The Fos gene family consists of 4 members: c-Fos, FosB, FosL1, and FosL2, also called Fos related antigen 1 and 2 (FRA1 and 2). These leucine zipper proteins can dimerize with proteins of the Jun family leading to the formation of the transcription factor complex AP1 (1).
The expression of Fos proteins is rapidly and transiently induced by different extracellular stimuli such as growth factors, cytokines, neurotransmitters, polypeptide hormones and stress (2).
In addition Fos proteins can be phosphorylated by ERK kinases modulating transcriptional activity, protein stability and localization (3). c-Fos is the homologue to the Finkel-Biskis-Jinkins (FBJ) murine osteosarcoma virus oncogene (4).

1 ) Kovary K et al., Mol. Cell. Biol. (1992)
2 ) Greenberg ME et al., Nature ()
3 ) Monje P et al., J. Biol. Chem. (2005)
4 ) Müller-Ladner U et al., Rheum. Dis. Clin. North Am. (1995)

Protocols

ICC

IHC

IHC-P

OTHER

Preabsorbtion

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