Synaptic Systems - GABA-A receptor alpha6

Cat. No.: 224 603

Amount: 50 µg

Price: $375.00

Cat. No. 224 603	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 20 to 30 from rat GABA-A receptor α6 (UniProt Id: P30191)
Reactivity	Reacts with: rat (P30191), mouse (P16305). Other species not tested yet.
Remarks	WB: GABA-A receptor alpha6 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Data sheet	224_603.pdf

References for GABA-A receptor alpha6 - 224 603

Neurexophilin4 is a selectively expressed α-neurexin ligand that modulates specific cerebellar synapses and motor functions.
Meng X, McGraw CM, Wang W, Jing J, Yeh SY, Wang L, Lopez J, Brown AM, Lin T, Chen W, Xue M, et al.
eLife (2019) 8: . 224 603 WB, IHC; tested species: mouse

Gene expression alterations in the cerebellum and granule neurons of Cstb(-/-) mouse are associated with early synaptic changes and inflammation.
Joensuu T, Tegelberg S, Reinmaa E, Segerstråle M, Hakala P, Pehkonen H, Korpi ER, Tyynelä J, Taira T, Hovatta I, Kopra O, et al.
PloS one (2014) 92: e89321. 224 603 IHC, WB

Development and Optimization of a Multilayer Rat Purkinje Neuron Culture.
Uggerud IM, Kråkenes T, Hirai H, Vedeler CA, Schubert M
Cerebellum (London, England) (2023) : . 224 603 ICC; tested species: rat

Encephalitis patient-derived monoclonal GABAA receptor antibodies cause epileptic seizures.
Kreye J, Wright SK, van Casteren A, Stöffler L, Machule ML, Reincke SM, Nikolaus M, van Hoof S, Sanchez-Sendin E, Homeyer MA, Cordero Gómez C, et al.
The Journal of experimental medicine (2021) 21811: . 224 603 ICC; tested species: rat

Granule cells constitute one of the major neuronal subtypes in the molecular layer of the posterior cerebellum.
Dey MR, Reddy K, Yoshida H, Nishiyama N, Zemelman B, Nishiyama H
eNeuro (2022) : . 224 603 IHC; tested species: mouse

A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 224 603 IHC; tested species: rat

Cat. No.: 224 603

Amount: 50 µg

Price: $375.00

ICC

IHC

Background

Gamma-aminobutyric acid type A (GABA-A) receptors mediate the majority of inhibitory neurotransmission in the brain. These receptor proteins are ligand gated chloride ion channels and consist of a pentameric combination of different subunits (alpha, beta, gamma, delta, epsilon and rho). The resulting heterogenous population of GABA-A receptor subtypes are expressed throughout the brain with specific cellular and subcellular expression patterns.

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5 ) Sassoè-Pognetto M et al., Ann. N. Y. Acad. Sci. (2009)
6 ) Viltono L et al., J. Comp. Neurol. (2008)
7 ) Kralic JE et al., J. Comp. Neurol. (2006)
8 ) Essrich C et al., Nat. Neurosci. (1998)
9 ) Fritschy JM et al., J. Comp. Neurol. (1995)
10 ) Benke D et al., J. Biol. Chem. (1994)

Protocols

ICC

IHC

GABA-A receptor alpha6 antibody extracellular - 224 603

References for GABA-A receptor alpha6 - 224 603