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GABA-A receptor alpha1 antibody extracellular - 224 203 K.O.

GABA-A receptors are involved in inhibitory neurotransmission
Rabbit polyclonal purified antibody
Cat. No.: 224 203
Amount: 50 µg
Price: $380.00
Cat. No. 224 203 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 500 (see remarks) gallery  
IHC: 1 : 1000 up to 1 : 5000 (see remarks) gallery  
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Synthetic peptide/recombinant protein corresponding to residues near the amino terminus from rat GABA-A receptor α1. (UniProt Id: P62813)
Reactivity Reacts with: rat (P62813), mouse (P62812).
Other species not tested yet.
Specificity K.O. validated PubMed: 25080596
Matching control protein/peptide 224-2P
Remarks

WB:  GABA-A receptor alpha1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
ICC: This antibody is PFA fixation sensitive, use only mild fixation (2% PFA). Best results are obtained by application on living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondaryreagents.
IHC: For best results use the protocol of Schneider Gasser et al., 2006.
Data sheet 224_203.pdf

References for GABA-A receptor alpha1 - 224 203

The endoplasmic reticulum membrane complex promotes proteostasis of GABAA receptors.
Whittsette AL, Wang YJ, Mu TW
iScience (2022) 258: 104754. 224 203 WB, IP, UPTAKE; tested species: rat
Selective disruption of inhibitory synapses leading to neuronal hyperexcitability at an early stage of tau pathogenesis in a mouse model.
Shimojo M, Takuwa H, Takado Y, Tokunaga M, Tsukamoto S, Minatohara K, Ono M, Seki C, Maeda J, Urushihata T, Minamihisamatsu T, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 224 203 WB; tested species: mouse
Enriched environment ameliorates memory impairments in rats after postsurgery sleep deprivation.
Gao J, Yang C, Li D, Zhao L, Wang H
Journal of chemical neuroanatomy (2020) : 101850. 224 203 WB, IHC; tested species: rat
Network Neuromodulation of Opioid and GABAergic Receptors Following a Combination of "Juvenile" and "Adult Stress" in Rats.
Horovitz O, Ardi Z, Ashkenazi SK, Ritov G, Anunu R, Richter-Levin G
International journal of molecular sciences (2020) 2115: . 224 203 WB; tested species: rat
The role of the GABAA receptor Alpha 1 subunit in the ventral hippocampus in stress resilience.
Ardi Z, Richter-Levin A, Xu L, Cao X, Volkmer H, Stork O, Richter-Levin G
Scientific reports (2019) 91: 13513. 224 203 WB; tested species: rat
Neurexophilin4 is a selectively expressed α-neurexin ligand that modulates specific cerebellar synapses and motor functions.
Meng X, McGraw CM, Wang W, Jing J, Yeh SY, Wang L, Lopez J, Brown AM, Lin T, Chen W, Xue M, et al.
eLife (2019) 8: . 224 203 WB, IHC; tested species: mouse
Chronic Toxoplasma infection is associated with distinct alterations in the synaptic protein composition.
Lang D, Schott BH, van Ham M, Morton L, Kulikovskaja L, Herrera-Molina R, Pielot R, Klawonn F, Montag D, Jänsch L, Gundelfinger ED, et al.
Journal of neuroinflammation (2018) 151: 216. 224 203 WB, IHC; tested species: mouse
Behavioral profiling as a translational approach in an animal model of posttraumatic stress disorder.
Ardi Z, Albrecht A, Richter-Levin A, Saha R, Richter-Levin G
Neurobiology of disease (2016) 88: 139-47. 224 203 WB
Different subtypes of GABA-A receptors are expressed in human, mouse and rat T lymphocytes.
Mendu SK, Bhandage A, Jin Z, Birnir B
PloS one (2012) 78: e42959. 224 203 WB, ICC
The endoplasmic reticulum membrane complex promotes proteostasis of GABAA receptors.
Whittsette AL, Wang YJ, Mu TW
iScience (2022) 258: 104754. 224 203 WB, IP, UPTAKE; tested species: rat
Distinct mechanisms drive sequential internalization and degradation of GABAARs during global ischemia and reperfusion injury.
Garcia JD, Wolfe SE, Stewart AR, Tiemeier E, Gookin SE, Guerrero MB, Quillinan N, Smith KR
iScience (2023) 2610: 108061. 224 203 ICC; tested species: rat
Conformational state-dependent regulation of GABAA receptor diffusion and subsynaptic domains.
Merlaud Z, Marques X, Russeau M, Saade U, Tostain M, Moutkine I, Gielen M, Corringer PJ, Lévi S
iScience (2022) 2511: 105467. 224 203 ICC; tested species: rat
ErbB4 promotes inhibitory synapse formation by cell adhesion, independent of its kinase activity.
Luo B, Liu Z, Lin D, Chen W, Ren D, Yu Z, Xiong M, Zhao C, Fei E, Li B
Translational psychiatry (2021) 111: 361. 224 203 ICC; tested species: mouse
Stepwise disassembly of GABAergic synapses during pathogenic excitotoxicity.
Garcia JD, Gookin SE, Crosby KC, Schwartz SL, Tiemeier E, Kennedy MJ, Dell'Acqua ML, Herson PS, Quillinan N, Smith KR
Cell reports (2021) 3712: 110142. 224 203 ICC; tested species: rat
Local miRNA-Dependent Translational Control of GABAAR Synthesis during Inhibitory Long-Term Potentiation.
Rajgor D, Purkey AM, Sanderson JL, Welle TM, Garcia JD, Dell'Acqua ML, Smith KR
Cell reports (2020) 3112: 107785. 224 203 ICC; tested species: rat
Essential role for InSyn1 in dystroglycan complex integrity and cognitive behaviors in mice.
Uezu A, Hisey E, Kobayashi Y, Gao Y, Bradshaw TW, Devlin P, Rodriguiz R, Tata PR, Soderling S
eLife (2019) 8: . 224 203 ICC; tested species: mouse
Structure of excitatory synapses and GABAA receptor localization at inhibitory synapses are regulated by neuroplastin-65.
Herrera-Molina R, Sarto-Jackson I, Montenegro-Venegas C, Heine M, Smalla KH, Seidenbecher CI, Beesley PW, Gundelfinger ED, Montag D
The Journal of biological chemistry (2014) 28913: 8973-88. 224 203 ICC; tested species: mouse
Different subtypes of GABA-A receptors are expressed in human, mouse and rat T lymphocytes.
Mendu SK, Bhandage A, Jin Z, Birnir B
PloS one (2012) 78: e42959. 224 203 WB, ICC
Developmental transformation of Ca2+ channel-vesicle nanotopography at a central GABAergic synapse.
Chen JJ, Kaufmann WA, Chen C, Arai I, Kim O, Shigemoto R, Jonas P
Neuron (2024) 1125: 755-771.e9. 224 203 IHC; tested species: mouse
Vision-dependent specification of cell types and function in the developing cortex.
Cheng S, Butrus S, Tan L, Xu R, Sagireddy S, Trachtenberg JT, Shekhar K, Zipursky SL
Cell (2022) 1852: 311-327.e24. 224 203 IHC; tested species: mouse
Enhancing neuronal chloride extrusion rescues α2/α3 GABAA-mediated analgesia in neuropathic pain.
Lorenzo LE, Godin AG, Ferrini F, Bachand K, Plasencia-Fernandez I, Labrecque S, Girard AA, Boudreau D, Kianicka I, Gagnon M, Doyon N, et al.
Nature communications (2020) 111: 869. 224 203 IHC; tested species: rat
Enriched environment ameliorates memory impairments in rats after postsurgery sleep deprivation.
Gao J, Yang C, Li D, Zhao L, Wang H
Journal of chemical neuroanatomy (2020) : 101850. 224 203 WB, IHC; tested species: rat
Neurexophilin4 is a selectively expressed α-neurexin ligand that modulates specific cerebellar synapses and motor functions.
Meng X, McGraw CM, Wang W, Jing J, Yeh SY, Wang L, Lopez J, Brown AM, Lin T, Chen W, Xue M, et al.
eLife (2019) 8: . 224 203 WB, IHC; tested species: mouse
IgSF9b regulates anxiety behaviors through effects on centromedial amygdala inhibitory synapses.
Babaev O, Cruces-Solis H, Piletti Chatain C, Hammer M, Wenger S, Ali H, Karalis N, de Hoz L, Schlüter OM, Yanagawa Y, Ehrenreich H, et al.
Nature communications (2018) 91: 5400. 224 203 IHC; tested species: mouse
Chronic Toxoplasma infection is associated with distinct alterations in the synaptic protein composition.
Lang D, Schott BH, van Ham M, Morton L, Kulikovskaja L, Herrera-Molina R, Pielot R, Klawonn F, Montag D, Jänsch L, Gundelfinger ED, et al.
Journal of neuroinflammation (2018) 151: 216. 224 203 WB, IHC; tested species: mouse
Spatiotemporal Distribution of GABAA Receptor Subunits Within Layer II of Mouse Medial Entorhinal Cortex: Implications for Grid Cell Excitability.
Berggaard N, Seifi M, van der Want JJL, Swinny JD
Frontiers in neuroanatomy (2018) 12: 46. 224 203 IHC; tested species: mouse
Iterative expansion microscopy.
Chang JB, Chen F, Yoon YG, Jung EE, Babcock H, Kang JS, Asano S, Suk HJ, Pak N, Tillberg PW, Wassie AT, et al.
Nature methods (2017) 146: 593-599. 224 203 IHC; tested species: mouse
Structural and functional characterization of dendritic arbors and GABAergic synaptic inputs on interneurons and principal cells in the rat basolateral amygdala.
Klenowski PM, Fogarty MJ, Belmer A, Noakes PG, Bellingham MC, Bartlett SE
Journal of neurophysiology (2015) 1142: 942-57. 224 203 IHC
Synaptic recruitment of gephyrin regulates surface GABAA receptor dynamics for the expression of inhibitory LTP.
Petrini EM, Ravasenga T, Hausrat TJ, Iurilli G, Olcese U, Racine V, Sibarita JB, Jacob TC, Moss SJ, Benfenati F, Medini P, et al.
Nature communications (2014) 5: 3921. 224 203 IHC; tested species: mouse
Differential GABAergic and glycinergic inputs of inhibitory interneurons and Purkinje cells to principal cells of the cerebellar nuclei.
Husson Z, Rousseau CV, Broll I, Zeilhofer HU, Dieudonné S
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3428: 9418-31. 224 203 IHC; tested species: mouse
Molecular and functional diversity of GABA-A receptors in the enteric nervous system of the mouse colon.
Seifi M, Brown JF, Mills J, Bhandari P, Belelli D, Lambert JJ, Rudolph U, Swinny JD
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3431: 10361-78. 224 203 IHC; KO verified; tested species: mouse
Epitope-preserving magnified analysis of proteome (eMAP).
Park J, Khan S, Yun DH, Ku T, Villa KL, Lee JE, Zhang Q, Park J, Feng G, Nedivi E, Chung K, et al.
Science advances (2021) 746: eabf6589. 224 203 Clarity; tested species: mouse,marmoset
The endoplasmic reticulum membrane complex promotes proteostasis of GABAA receptors.
Whittsette AL, Wang YJ, Mu TW
iScience (2022) 258: 104754. 224 203 WB, IP, UPTAKE; tested species: rat
Cat. No.: 224 203
Amount: 50 µg
Price: $380.00
The endoplasmic reticulum membrane complex promotes proteostasis of GABAA receptors.
Whittsette AL, Wang YJ, Mu TW
iScience (2022) 258: 104754. 224 203 WB, IP, UPTAKE; tested species: rat
Selective disruption of inhibitory synapses leading to neuronal hyperexcitability at an early stage of tau pathogenesis in a mouse model.
Shimojo M, Takuwa H, Takado Y, Tokunaga M, Tsukamoto S, Minatohara K, Ono M, Seki C, Maeda J, Urushihata T, Minamihisamatsu T, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 224 203 WB; tested species: mouse
Enriched environment ameliorates memory impairments in rats after postsurgery sleep deprivation.
Gao J, Yang C, Li D, Zhao L, Wang H
Journal of chemical neuroanatomy (2020) : 101850. 224 203 WB, IHC; tested species: rat
Network Neuromodulation of Opioid and GABAergic Receptors Following a Combination of "Juvenile" and "Adult Stress" in Rats.
Horovitz O, Ardi Z, Ashkenazi SK, Ritov G, Anunu R, Richter-Levin G
International journal of molecular sciences (2020) 2115: . 224 203 WB; tested species: rat
The role of the GABAA receptor Alpha 1 subunit in the ventral hippocampus in stress resilience.
Ardi Z, Richter-Levin A, Xu L, Cao X, Volkmer H, Stork O, Richter-Levin G
Scientific reports (2019) 91: 13513. 224 203 WB; tested species: rat
Neurexophilin4 is a selectively expressed α-neurexin ligand that modulates specific cerebellar synapses and motor functions.
Meng X, McGraw CM, Wang W, Jing J, Yeh SY, Wang L, Lopez J, Brown AM, Lin T, Chen W, Xue M, et al.
eLife (2019) 8: . 224 203 WB, IHC; tested species: mouse
Chronic Toxoplasma infection is associated with distinct alterations in the synaptic protein composition.
Lang D, Schott BH, van Ham M, Morton L, Kulikovskaja L, Herrera-Molina R, Pielot R, Klawonn F, Montag D, Jänsch L, Gundelfinger ED, et al.
Journal of neuroinflammation (2018) 151: 216. 224 203 WB, IHC; tested species: mouse
Behavioral profiling as a translational approach in an animal model of posttraumatic stress disorder.
Ardi Z, Albrecht A, Richter-Levin A, Saha R, Richter-Levin G
Neurobiology of disease (2016) 88: 139-47. 224 203 WB
Different subtypes of GABA-A receptors are expressed in human, mouse and rat T lymphocytes.
Mendu SK, Bhandage A, Jin Z, Birnir B
PloS one (2012) 78: e42959. 224 203 WB, ICC
The endoplasmic reticulum membrane complex promotes proteostasis of GABAA receptors.
Whittsette AL, Wang YJ, Mu TW
iScience (2022) 258: 104754. 224 203 WB, IP, UPTAKE; tested species: rat
Distinct mechanisms drive sequential internalization and degradation of GABAARs during global ischemia and reperfusion injury.
Garcia JD, Wolfe SE, Stewart AR, Tiemeier E, Gookin SE, Guerrero MB, Quillinan N, Smith KR
iScience (2023) 2610: 108061. 224 203 ICC; tested species: rat
Conformational state-dependent regulation of GABAA receptor diffusion and subsynaptic domains.
Merlaud Z, Marques X, Russeau M, Saade U, Tostain M, Moutkine I, Gielen M, Corringer PJ, Lévi S
iScience (2022) 2511: 105467. 224 203 ICC; tested species: rat
ErbB4 promotes inhibitory synapse formation by cell adhesion, independent of its kinase activity.
Luo B, Liu Z, Lin D, Chen W, Ren D, Yu Z, Xiong M, Zhao C, Fei E, Li B
Translational psychiatry (2021) 111: 361. 224 203 ICC; tested species: mouse
Stepwise disassembly of GABAergic synapses during pathogenic excitotoxicity.
Garcia JD, Gookin SE, Crosby KC, Schwartz SL, Tiemeier E, Kennedy MJ, Dell'Acqua ML, Herson PS, Quillinan N, Smith KR
Cell reports (2021) 3712: 110142. 224 203 ICC; tested species: rat
Local miRNA-Dependent Translational Control of GABAAR Synthesis during Inhibitory Long-Term Potentiation.
Rajgor D, Purkey AM, Sanderson JL, Welle TM, Garcia JD, Dell'Acqua ML, Smith KR
Cell reports (2020) 3112: 107785. 224 203 ICC; tested species: rat
Essential role for InSyn1 in dystroglycan complex integrity and cognitive behaviors in mice.
Uezu A, Hisey E, Kobayashi Y, Gao Y, Bradshaw TW, Devlin P, Rodriguiz R, Tata PR, Soderling S
eLife (2019) 8: . 224 203 ICC; tested species: mouse
Structure of excitatory synapses and GABAA receptor localization at inhibitory synapses are regulated by neuroplastin-65.
Herrera-Molina R, Sarto-Jackson I, Montenegro-Venegas C, Heine M, Smalla KH, Seidenbecher CI, Beesley PW, Gundelfinger ED, Montag D
The Journal of biological chemistry (2014) 28913: 8973-88. 224 203 ICC; tested species: mouse
Different subtypes of GABA-A receptors are expressed in human, mouse and rat T lymphocytes.
Mendu SK, Bhandage A, Jin Z, Birnir B
PloS one (2012) 78: e42959. 224 203 WB, ICC
Developmental transformation of Ca2+ channel-vesicle nanotopography at a central GABAergic synapse.
Chen JJ, Kaufmann WA, Chen C, Arai I, Kim O, Shigemoto R, Jonas P
Neuron (2024) 1125: 755-771.e9. 224 203 IHC; tested species: mouse
Vision-dependent specification of cell types and function in the developing cortex.
Cheng S, Butrus S, Tan L, Xu R, Sagireddy S, Trachtenberg JT, Shekhar K, Zipursky SL
Cell (2022) 1852: 311-327.e24. 224 203 IHC; tested species: mouse
Enhancing neuronal chloride extrusion rescues α2/α3 GABAA-mediated analgesia in neuropathic pain.
Lorenzo LE, Godin AG, Ferrini F, Bachand K, Plasencia-Fernandez I, Labrecque S, Girard AA, Boudreau D, Kianicka I, Gagnon M, Doyon N, et al.
Nature communications (2020) 111: 869. 224 203 IHC; tested species: rat
Enriched environment ameliorates memory impairments in rats after postsurgery sleep deprivation.
Gao J, Yang C, Li D, Zhao L, Wang H
Journal of chemical neuroanatomy (2020) : 101850. 224 203 WB, IHC; tested species: rat
Neurexophilin4 is a selectively expressed α-neurexin ligand that modulates specific cerebellar synapses and motor functions.
Meng X, McGraw CM, Wang W, Jing J, Yeh SY, Wang L, Lopez J, Brown AM, Lin T, Chen W, Xue M, et al.
eLife (2019) 8: . 224 203 WB, IHC; tested species: mouse
IgSF9b regulates anxiety behaviors through effects on centromedial amygdala inhibitory synapses.
Babaev O, Cruces-Solis H, Piletti Chatain C, Hammer M, Wenger S, Ali H, Karalis N, de Hoz L, Schlüter OM, Yanagawa Y, Ehrenreich H, et al.
Nature communications (2018) 91: 5400. 224 203 IHC; tested species: mouse
Chronic Toxoplasma infection is associated with distinct alterations in the synaptic protein composition.
Lang D, Schott BH, van Ham M, Morton L, Kulikovskaja L, Herrera-Molina R, Pielot R, Klawonn F, Montag D, Jänsch L, Gundelfinger ED, et al.
Journal of neuroinflammation (2018) 151: 216. 224 203 WB, IHC; tested species: mouse
Spatiotemporal Distribution of GABAA Receptor Subunits Within Layer II of Mouse Medial Entorhinal Cortex: Implications for Grid Cell Excitability.
Berggaard N, Seifi M, van der Want JJL, Swinny JD
Frontiers in neuroanatomy (2018) 12: 46. 224 203 IHC; tested species: mouse
Iterative expansion microscopy.
Chang JB, Chen F, Yoon YG, Jung EE, Babcock H, Kang JS, Asano S, Suk HJ, Pak N, Tillberg PW, Wassie AT, et al.
Nature methods (2017) 146: 593-599. 224 203 IHC; tested species: mouse
Structural and functional characterization of dendritic arbors and GABAergic synaptic inputs on interneurons and principal cells in the rat basolateral amygdala.
Klenowski PM, Fogarty MJ, Belmer A, Noakes PG, Bellingham MC, Bartlett SE
Journal of neurophysiology (2015) 1142: 942-57. 224 203 IHC
Synaptic recruitment of gephyrin regulates surface GABAA receptor dynamics for the expression of inhibitory LTP.
Petrini EM, Ravasenga T, Hausrat TJ, Iurilli G, Olcese U, Racine V, Sibarita JB, Jacob TC, Moss SJ, Benfenati F, Medini P, et al.
Nature communications (2014) 5: 3921. 224 203 IHC; tested species: mouse
Differential GABAergic and glycinergic inputs of inhibitory interneurons and Purkinje cells to principal cells of the cerebellar nuclei.
Husson Z, Rousseau CV, Broll I, Zeilhofer HU, Dieudonné S
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3428: 9418-31. 224 203 IHC; tested species: mouse
Molecular and functional diversity of GABA-A receptors in the enteric nervous system of the mouse colon.
Seifi M, Brown JF, Mills J, Bhandari P, Belelli D, Lambert JJ, Rudolph U, Swinny JD
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3431: 10361-78. 224 203 IHC; KO verified; tested species: mouse
Epitope-preserving magnified analysis of proteome (eMAP).
Park J, Khan S, Yun DH, Ku T, Villa KL, Lee JE, Zhang Q, Park J, Feng G, Nedivi E, Chung K, et al.
Science advances (2021) 746: eabf6589. 224 203 Clarity; tested species: mouse,marmoset
The endoplasmic reticulum membrane complex promotes proteostasis of GABAA receptors.
Whittsette AL, Wang YJ, Mu TW
iScience (2022) 258: 104754. 224 203 WB, IP, UPTAKE; tested species: rat
WB
ICC
IHC
IHC
Background

Gamma-aminobutyric acid type A (GABA-A) receptors mediate the majority of inhibitory neurotransmission in the brain. These receptor proteins are ligand gated chloride ion channels and consist of a pentameric combination of different subunits (alpha, beta, gamma, delta, epsilon and rho). The resulting heterogenous population of GABA-A receptor subtypes are expressed throughout the brain with specific cellular and subcellular expression patterns.
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