Synaptic Systems - GABA-A receptor alpha2

Cat. No.: 224 104

Amount: 100 µl

Price: $365.00

Cat. No. 224 104	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: yes ICC: 1 : 200 up to 1 : 500 (see remarks) gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 29 to 37 from rat GABA-A receptor α2 (UniProt Id: P23576)
Reactivity	Reacts with: human (P47869), rat (P23576), mouse (P26048). Other species not tested yet.
Matching control protein/peptide	224-1P
Remarks	WB: GABA-A receptor alpha2 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. ICC: This antibody is also suitable for the surface staining of living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondary reagents.
Data sheet	224_104.pdf

References for GABA-A receptor alpha2 - 224 104

γ-Aminobutyric Acid Type A (GABAA) Receptor Subunits Play a Direct Structural Role in Synaptic Contact Formation via Their N-terminal Extracellular Domains.
Brown LE, Nicholson MW, Arama JE, Mercer A, Thomson AM, Jovanovic JN
The Journal of biological chemistry (2016) 29127: 13926-42. 224 104 ICC

Structure of excitatory synapses and GABAA receptor localization at inhibitory synapses are regulated by neuroplastin-65.
Herrera-Molina R, Sarto-Jackson I, Montenegro-Venegas C, Heine M, Smalla KH, Seidenbecher CI, Beesley PW, Gundelfinger ED, Montag D
The Journal of biological chemistry (2014) 28913: 8973-88. 224 104 ICC; tested species: mouse

Loss of function of NCOR1 and NCOR2 impairs memory through a novel GABAergic hypothalamus-CA3 projection.
Zhou W, He Y, Rehman AU, Kong Y, Hong S, Ding G, Yalamanchili HK, Wan YW, Paul B, Wang C, Gong Y, et al.
Nature neuroscience (2019) : . 224 104 IHC; tested species: mouse

Activity-dependent mismatch between axo-axonic synapses and the axon initial segment controls neuronal output.
Wefelmeyer W, Cattaert D, Burrone J
Proceedings of the National Academy of Sciences of the United States of America (2015) 11231: 9757-62. 224 104 IHC

Fast Regulation of GABAAR Diffusion Dynamics by Nogo-A Signaling.
Fricke S, Metzdorf K, Ohm M, Haak S, Heine M, Korte M, Zagrebelsky M
Cell reports (2019) 293: 671-684.e6. 224 104 UPTAKE; tested species: mouse

Cat. No.: 224 104

Amount: 100 µl

Price: $365.00

ICC

Background

Gamma-aminobutyric acid type A (GABA-A) receptors mediate the majority of inhibitory neurotransmission in the brain. These receptor proteins are ligand gated chloride ion channels and consist of a pentameric combination of different subunits (alpha, beta, gamma, delta, epsilon and rho). The resulting heterogenous population of GABA-A receptor subtypes are expressed throughout the brain with specific cellular and subcellular expression patterns.

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6 ) Viltono L et al., J. Comp. Neurol. (2008)
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8 ) Essrich C et al., Nat. Neurosci. (1998)
9 ) Fritschy JM et al., J. Comp. Neurol. (1995)
10 ) Benke D et al., J. Biol. Chem. (1994)

Protocols

Standard protocol

ICC

OTHER

Preabsorbtion

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GABA-A receptor alpha2 antibody extracellular - 224 104

References for GABA-A receptor alpha2 - 224 104