Synaptic Systems - Abeta-pE11

Cat. No.: 218 603

Amount: 50 µg

Price: $375.00

The Arctic AβPP mutation leads to Alzheimer's disease pathology with highly variable topographic deposition of differentially truncated Aβ.
Kalimo H, Lalowski M, Bogdanovic N, Philipson O, Bird TD, Nochlin D, Schellenberg GD, Brundin R, Olofsson T, Soliymani R, Baumann M, et al.
Acta neuropathologica communications (2013) 1: 60. 218 603 IHC; tested species: human

WB

IHC

IHC-P

ELISA

Background

Amyloid deposits, also called plaques, of Alzheimer's patients consist of several protein components like the amyloid beta-peptides (Abeta, Aβ) 1-40/42 and additional C- and N-terminally truncated and modified fragments. Very abundant are the isoaspartate (isoAsp)-Abeta and pyroglutamyl (pGlu)-Abeta peptides. The latter are formed by cyclization of the N-terminal glutamate at position 3 or 11 catalyzed by glutaminyl cyclase (QC) resulting in very amyloidogenic and neurotxic variants of Abeta; Abeta pE3 and Abeta-pE11.
In contrast to extracellular plaques that do not perfectly correlate with Alzheimer´s disease intraneuronal Abeta accumulation and vascular Abeta deposits have gained more and more evidence to be among the crucial factors responsible for progressive neuron loss.

1 ) Sullivan CP et al., Neurosci. Lett. (2011)
2 ) Perez-Garmendia R et al., J. Neuroimmunol. (2010)
3 ) Schilling S et al., Nat. Med. (2008)
4 ) Hook V et al., Biol. Chem. (2008)
5 ) Böhme L et al., Biol. Chem. (2008)
6 ) Schilling S et al., J. Neurochem. (2008)
7 ) Wiltfang J et al., J. Biol. Chem. (2001)
8 ) Wood SJ et al., J. Mol. Biol. (1996)
9 ) Kuo YM et al., J. Biol. Chem. (1996)

Protocols

WB

IHC

IHC-P

ELISA

Standard protocol

Other products for this target

Cat. No. 218 603	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (see remarks) gallery IP: not tested yet ICC: not tested yet IHC: 1 : 200 (see remarks) gallery IHC-P: 1 : 100 (see remarks) gallery ELISA: yes (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Immunogen	Synthetic peptide corresponding to AA 11 to 16 from human Abeta-pE11 (UniProt Id: P05067)
Reactivity	Reacts with: human (P05067), rat (P08592), mouse (P12023). Other species not tested yet.
Specificity	Specific for Abeta-pE11.
Remarks	WB: Detects purified Abeta-pE11. complex samples like brain extracts not tested yet. Boil membrane after blotting for 3min. IHC: Antigen retrieval with formic acid is required. IHC-P: Antigen retrieval with formic acid is required. ELISA: Suitable as detector antibody.
Data sheet	218_603.pdf

Abeta-pE11 antibody - 218 603

References for Abeta-pE11 - 218 603