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Cat. No. 216 011 |
100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
Applications | |
Clone | 62-2E8 |
Subtype | IgG1 (κ light chain) |
Immunogen | Recombinant protein corresponding to residues near the C-terminus of rat ChT (UniProt Id: Q9JMD7) |
Reactivity |
Reacts with: monkey, rat (Q9JMD7), mouse (Q8BGY9). Other species not tested yet. |
Specificity | K.O. validated |
Remarks |
WB: To avoid protein aggregation, do not heat samples for SDS-PAGE. |
Data sheet | 216_011.pdf |
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Acetylcholine (ACh) functions as a neurotransmitter in both the central and peripheral nervous systems of all vertebrates, and is the principal neurotransmitter used at the neuromuscular junction. This neurotransmitter is synthesized from choline (Ch) and acetyl-coenzyme A by choline acetyltransferase (ChAT). For this pathway choline is required, which neurons acquire through high-affinity choline transporters (ChTs). ChT have been found on the presynaptic membrane but also on ACh containing synaptic vesicles.