Synaptic Systems

Cat. No.: 216 011

Amount: 100 µg

Price: $420.00

Cat. No. 216 011	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: yes ICC: not tested yet IHC: 1 : 100 gallery IHC-P: 1 : 1000 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	62-2E8
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to residues near the C-terminus of rat ChT (UniProt Id: Q9JMD7)
Reactivity	Reacts with: monkey, rat (Q9JMD7), mouse (Q8BGY9). Other species not tested yet.
Specificity	K.O. validated
Remarks	WB: ChT aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. IHC-P: Not recommended for rat tissue. Unspecific nuclear signal has been observed using our standard IHC-P protocol.
Data sheet	216_011.pdf

References for ChT - 216 011

Aberrant trafficking of the high-affinity choline transporter in AP-3-deficient mice.
Misawa H, Fujigaya H, Nishimura T, Moriwaki Y, Okuda T, Kawashima K, Nakata K, Ruggiero AM, Blakely RD, Nakatsu F, Ohno H, et al.
The European journal of neuroscience (2008) 2712: 3109-17. 216 011 WB

Vesicular localization and activity-dependent trafficking of presynaptic choline transporters.
Ferguson SM, Savchenko V, Apparsundaram S, Zwick M, Wright J, Heilman CJ, Yi H, Levey AI, Blakely RD
The Journal of neuroscience : the official journal of the Society for Neuroscience (2003) 2330: 9697-709. 216 011 WB, IP, IHC; tested species: mouse

Central Cholinergic Synapse Formation in Optimized Primary Septal-Hippocampal Co-cultures.
Djemil S, Ressel CR, Abdel-Ghani M, Schneeweis AK, Pak DTS
Cellular and molecular neurobiology (2020) : . 216 011 ICC; tested species: rat

Vesicular acetylcholine transporter (VAChT) over-expression induces major modifications of striatal cholinergic interneuron morphology and function.
Janickova H, Prado VF, Prado MAM, El Mestikawy S, Bernard V
Journal of neurochemistry (2017) : . 216 011 IHC; tested species: mouse

Distribution of high affinity choline transporter immunoreactivity in the primate central nervous system.
Kus L, Borys E, Ping Chu Y, Ferguson SM, Blakely RD, Emborg ME, Kordower JH, Levey AI, Mufson EJ
The Journal of comparative neurology (2003) 4633: 341-57. 216 011 IHC; tested species: monkey

Cat. No.: 216 011

Amount: 100 µg

Price: $420.00

IHC

IHC-P

Background

Acetylcholine (ACh) functions as a neurotransmitter in both the central and peripheral nervous systems of all vertebrates, and is the principal neurotransmitter used at the neuromuscular junction. This neurotransmitter is synthesized from choline (Ch) and acetyl-coenzyme A by choline acetyltransferase (ChAT). For this pathway choline is required, which neurons acquire through high-affinity choline transporters (ChTs). ChT have been found on the presynaptic membrane but also on ACh containing synaptic vesicles.

1 ) Madziar B et al., J. Neurochem. (2008)
2 ) Ribeiro FM et al., Eur. J. Neurosci. (2007)
3 ) Ferguson SM et al., Mol. Interv. (2004)
4 ) Ferguson SM et al., J. Neurosci. (2003)
5 ) Knipper M et al., Biochim. Biophys. Acta (1991)

Protocols

Standard protocol

IHC

IHC-P

Other products for this target

ChT antibody - 216 011 K.O.

References for ChT - 216 011