ChT antibody - 216 004

ChT is a high affinity choline transporter

Guinea pig polyclonal antiserum

Cat. No.: 216 004

Amount: 100 µl

Price: $365.00

Cat. No. 216 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: not recommended IHC-P: 1 : 200 up to 1 : 1000 gallery IHC-Fr: not recommended
Immunogen	Recombinant protein corresponding to AA 501 to 581 from rat ChT (UniProt Id: Q9JMD7)
Reactivity	Reacts with: rat (Q9JMD7). Weaker signal: mouse (Q8BGY9). Other species not tested yet.
Data sheet	216_004.pdf

Cat. No.: 216 004

Amount: 100 µl

Price: $365.00

IHC-P

Background

Acetylcholine (ACh) functions as a neurotransmitter in both the central and peripheral nervous systems of all vertebrates, and is the principle neurotransmitter used at the neuromuscular junction. This neurotransmitter is synthesized from choline (Ch) and acetyl-coenzyme A by choline acetyltransferase (ChAT). For this pathway choline is required, which neurons acquire through high-affinity choline transporters (ChTs). ChT have been found on the presynaptic membrane but also on ACh containing synaptic vesicles.

1 ) Madziar B, Journal of neurochemistry (2008) 1075: 1284-93.
2 ) Ribeiro FM, The European journal of neuroscience (2007) 2612: 3437-48.
3 ) Ferguson SM, Molecular interventions (2004) 41: 22-37.
4 ) Ferguson SM, The Journal of neuroscience : the official journal of the Society for Neuroscience (2003) 2330: 9697-709.
5 ) Knipper M, Biochimica et biophysica acta (1991) 10652: 107-13.

Protocols

IHC-P

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