ChT antibody - 216 004

ChT is a high affinity choline transporter

Guinea pig polyclonal antiserum

Cat. No.: 216 004

Amount: 100 µl

Price: $365.00

Cat. No. 216 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: not recommended IHC-P: 1 : 200 up to 1 : 1000 gallery IHC-Fr: not recommended Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 501 to 581 from rat ChT (UniProt Id: Q9JMD7)
Reactivity	Reacts with: rat (Q9JMD7). Weaker signal: mouse (Q8BGY9). Other species not tested yet.
Data sheet	216_004.pdf

Cat. No.: 216 004

Amount: 100 µl

Price: $365.00

IHC-P

Background

Acetylcholine (ACh) functions as a neurotransmitter in both the central and peripheral nervous systems of all vertebrates, and is the principle neurotransmitter used at the neuromuscular junction. This neurotransmitter is synthesized from choline (Ch) and acetyl-coenzyme A by choline acetyltransferase (ChAT). For this pathway choline is required, which neurons acquire through high-affinity choline transporters (ChTs). ChT have been found on the presynaptic membrane but also on ACh containing synaptic vesicles.

1 ) Madziar B et al., J. Neurochem. (2008)
2 ) Ribeiro FM et al., Eur. J. Neurosci. (2007)
3 ) Ferguson SM et al., Mol. Interv. (2004)
4 ) Ferguson SM et al., J. Neurosci. (2003)
5 ) Knipper M et al., Biochim. Biophys. Acta (1991)

Protocols

IHC-P

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