Tyrosine hydroxylase antibody - 213 009

Tyrosine hydroxylase is the rate limiting enzyme in catecholamine synthesis

Chicken monoclonal recombinant IgY

Cat. No.: 213 009

Amount: 50 µg

Price: $420.00

Cat. No. 213 009	50 µg purified recombinant IgY, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 2000 (AP staining) (see remarks) gallery IP: not tested yet ICC: 1 : 10000 up to 1 : 20000 gallery IHC: 1 : 10000 up to 1 : 20000 (see remarks) gallery IHC-P: 1 : 5000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Ch103E1
Subtype	IgY (λ light chain)
Immunogen	Recombinant protein corresponding to residues near the amino-terminus of rat TyrH (UniProt Id: P04177)
Epitop	AA 65 to 163 from rat TyrH (UniProt Id: P04177)
Reactivity	Reacts with: mouse (P24529), rat (P04177). Other species not tested yet.
Remarks	This antibody is a chimeric antibody based on the monoclonal mouse antibody 103E1. The constant regions of the heavy and light chains have been replaced by chicken specific sequences. The antibody can therefore be used with standard anti-chicken secondary reagents. The antibody has been expressed in mammalian cells. WB: This antibody is less sensitive than our polyclonal antibodies (cat. no. 213 102 and 213 104). IHC: Heat-mediated antigen retrieval (citrate buffer pH 6) is required for immunohistochemical staining
Data sheet	213_009.pdf

Cat. No.: 213 009

Amount: 50 µg

Price: $420.00

ICC

IHC

IHC-P

Background

Tyrosine hydroxylase is one of the key enzymes in the synthesis pathway of catecholamines like adrenalin, noradrenalin and dopamin and is frequently used as a marker for dopaminergic neurons. This neuronal subpopulation is especially affected in Parkinson's disease.

1 ) Vadasz C et al., Neuroscience (2007)
2 ) Rao F et al., Circulation (2007)
3 ) Lehmann IT et al., J. Biol. Chem. (2006)
4 ) Peng X et al., J. Cell. Sci. (2005)
5 ) Jeon YK et al., Mol. Cells (2001)
6 ) McLean JH et al., J. Neurosci. (1988)
7 ) Musacchio JM et al., Proc. Natl. Acad. Sci. U.S.A. (1969)

Protocols

ICC

IHC

IHC-P

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