Synaptic Systems

Cat. No.: 202 018

Amount: 100 µg

Price: $415.00

Dot blot

Background

m6A (N6-methyladenosine) is a posttranscriptional RNA-modification found throughout all kingdoms, e.g. in vertebrate snRNAs U2, U4, U6, in viral and eukaryotic mRNAs, and in E. coli 16S rRNA.
Recent studies have found that mRNA is predominately m6A modified at stop codons and long internal exons, which are conserved between mouse and human. The so-called RNA methylome probably plays an important role in in the regulation of gene expression.
In E. coli Dam methylase introduces m6A modifications on the DNA level at the 5'-GATC-3' motif. This allows the cell to differentiate between the parental and the daughter strand during mismatch repair.

1 ) Bringmann P et al., FEBS Lett. (1987)
2 ) Warda AS et al., EMBO Rep. (2017)
3 ) Xiang Y et al., Nature (2017)
4 ) Koziol MJ et al., Bio Protoc (2016)
5 ) Koziol MJ et al., Nat. Struct. Mol. Biol. (2016)
6 ) Gokhale NS et al., Cell Host Microbe (2016)
7 ) Li X et al., Int J Mol Sci (2016)
8 ) Molinie B et al., Nat. Methods (2016)
9 ) Mishima E et al., PLoS ONE (2015)
10 ) Li Y et al., RNA (2015)

Protocols

IP

Standard protocol

MeRIP

m6A-sequencing / MeRIP-sequencing

Other products for this target

Cat. No. 202 018	100 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	Dot blot: 1 : 1000 up to 1 : 2000 gallery IP: yes MeRIP: yes Immunodetection of target molecules spotted onto a membrane. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Methylated RNA immunoprecipitation (MeRIP) sequencing is an antibody based m6A-mapping method, based on parallel sequencing of immunoisolated RNA fragments (100 -200 nt long) carrying m6A modifications.
Clone	Rb345E11
Subtype	IgG1 (κ light chain)
Immunogen	N6-methyladenosine fused to BSA.
Reactivity	Reacts with: human, rat, mouse, prokaryotes, eukaryotes. Other species not tested yet.
Specificity	Specific for N6-methyladenosine (m6A) with some cross-reactivity to m6Am
Remarks	This antibody is a chimeric antibody based on the well known monoclonal mouse antibody clone 345E11. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
Data sheet	202_018.pdf

m6A antibody - 202 018