Synaptic Systems

Cat. No.: 202 011

Amount: 100 µg

Price: $415.00

Cat. No. 202 011	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: (AP staining) suitable for WB and Dot Blot Dot blot: 1 : 1000 gallery IP: yes ICC: not tested yet IHC: not tested yet IHC-P: not tested yet MeRIP: yes Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunodetection of target molecules spotted onto a membrane. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Methylated RNA immunoprecipitation (MeRIP) sequencing is an antibody based m6A-mapping method, based on parallel sequencing of immunoisolated RNA fragments (100 -200 nt long) carrying m6A modifications.
Clone	345E11
Subtype	IgG2b (κ light chain)
Immunogen	N6-methyladenosine fused to BSA.
Reactivity	Reacts with: human, rat, mouse, eukaryotes, prokaryotes. Other species not tested yet.
Specificity	Specific for N6-methyladenosine (m6A) with some cross-reactivity to m6Am.
Data sheet	202_011.pdf

References for m6A - 202 011

N6-methyladenosine marks primary microRNAs for processing.
Alarcón CR, Lee H, Goodarzi H, Halberg N, Tavazoie SF
Nature (2015) 5197544: 482-5. 202 011 WB

Erasing m6A-dependent transcription signature of stress-sensitive genes triggers antidepressant actions.
Wu PF, Han QQ, Chen FF, Shen TT, Li YH, Cao Y, Chen JG, Wang F
Neurobiology of stress (2021) 15: 100390. 202 011 Dot blot, ICC; tested species: mouse

Temporal Control of Mammalian Cortical Neurogenesis by m6A Methylation.
Yoon KJ, Ringeling FR, Vissers C, Jacob F, Pokrass M, Jimenez-Cyrus D, Su Y, Kim NS, Zhu Y, Zheng L, Kim S, et al.
Cell (2017) 1714: 877-889.e17. 202 011 Dot blot; tested species: mouse

Single-nucleotide-resolution mapping of m6A and m6Am throughout the transcriptome.
Linder B, Grozhik AV, Olarerin-George AO, Meydan C, Mason CE, Jaffrey SR
Nature methods (2015) 128: 767-72. 202 011 Dot blot, MeRIP; tested species: human,mouse

Identification of Methylated Deoxyadenosines in Genomic DNA by dA6m DNA Immunoprecipitation.
Koziol MJ, Bradshaw CR, Allen GE, Costa AS, Frezza C
Bio-protocol (2016) 621: . 202 011 IP

Identification of methylated deoxyadenosines in vertebrates reveals diversity in DNA modifications.
Koziol MJ, Bradshaw CR, Allen GE, Costa ASH, Frezza C, Gurdon JB
Nature structural & molecular biology (2016) 231: 24-30. 202 011 IP

Role of 6mA in the Regulation of Metabolic Biosynthesis in Sorghum Callus.
Tian K, Liu C, Cai Y, Zhou C
Journal of agricultural and food chemistry (2024) 7234: 19232-19245. 202 011 MeRIP; tested species: human

Inhibition of YTHDF2 triggers proteotoxic cell death in MYC-driven breast cancer.
Einstein JM, Perelis M, Chaim IA, Meena JK, Nussbacher JK, Tankka AT, Yee BA, Li H, Madrigal AA, Neill NJ, Shankar A, et al.
Molecular cell (2021) 8115: 3048-3064.e9. 202 011 MeRIP; tested species: human

The topologies of N6 -Adenosine methylation (m6 A) in land plant mitochondria and their putative effects on organellar gene-expression.
Murik O, Chandran SA, Nevo-Dinur K, Sultan LD, Best C, Stein Y, Hazan C, Ostersetzer-Biran O
The Plant journal : for cell and molecular biology (2019) : . 202 011 MeRIP

Cat. No.: 202 011

Amount: 100 µg

Price: $415.00

N6-methyladenosine marks primary microRNAs for processing.
Alarcón CR, Lee H, Goodarzi H, Halberg N, Tavazoie SF
Nature (2015) 5197544: 482-5. 202 011 WB

Identification of Methylated Deoxyadenosines in Genomic DNA by dA6m DNA Immunoprecipitation.
Koziol MJ, Bradshaw CR, Allen GE, Costa AS, Frezza C
Bio-protocol (2016) 621: . 202 011 IP

Dot blot

Background

m6A (N6-methyladenosine) is a posttranscriptional RNA-modification found throughout all kingdoms, e.g. in vertebrate snRNAs U2, U4, U6, in viral and eukaryotic mRNAs, and in E. coli 16S rRNA.
Recent studies have found that mRNA is predominately m6A modified at stop codons and long internal exons, which are conserved between mouse and human. The so-called RNA methylome probably plays an important role in in the regulation of gene expression.
In E. coli Dam methylase introduces m6A modifications on the DNA level at the 5'-GATC-3' motif. This allows the cell to differentiate between the parental and the daughter strand during mismatch repair.

1 ) Bringmann P et al., FEBS Lett. (1987)
2 ) Warda AS et al., EMBO Rep. (2017)
3 ) Xiang Y et al., Nature (2017)
4 ) Koziol MJ et al., Bio Protoc (2016)
5 ) Koziol MJ et al., Nat. Struct. Mol. Biol. (2016)
6 ) Gokhale NS et al., Cell Host Microbe (2016)
7 ) Li X et al., Int J Mol Sci (2016)
8 ) Molinie B et al., Nat. Methods (2016)
9 ) Mishima E et al., PLoS ONE (2015)
10 ) Li Y et al., RNA (2015)

Protocols

m6A from nuclear extracts

MeRIP

m6A-sequencing / MeRIP-sequencing

Other products for this target

m6A antibody - 202 011

References for m6A - 202 011