Synaptic Systems - m3G-cap, m7G-cap

Cat. No.: 201 011

Amount: 100 µg

Price: $415.00

Cat. No. 201 011	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: yes ICC: yes (see reference) IHC: not tested yet IHC-P: not tested yet ELISA: yes Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Clone	H20
Subtype	IgG1 (κ light chain)
Immunogen	Synthetic m₃G-cap conjugated to human serum albumin.
Reactivity	Reacts with: human, rat, mouse, eukaryotes. Other species not tested yet.
Specificity	Recognizes m₃G-cap and m⁷G-cap.
Remarks	This antibody can be used to detect capped RNAs (e.g. in viruses) or to identify and purify proteins associated with capped RNAs (see reference #2).
Data sheet	201_011.pdf

References for m3G-cap, m7G-cap - 201 011

Capping pores of alphavirus nsP1 gate membranous viral replication factories.
Jones R, Bragagnolo G, Arranz R, Reguera J
Nature (2020) : . 201 011 WB

Approved drugs screening against the nsP1 capping enzyme of Venezuelan equine encephalitis virus using an immuno-based assay.
Ferreira-Ramos AS, Li C, Eydoux C, Contreras JM, Morice C, Quérat G, Gigante A, Pérez Pérez MJ, Jung ML, Canard B, Guillemot JC, et al.
Antiviral research (2019) : . 201 011 WB, ELISA

Development of RNA aptamer that inhibits methyltransferase activity of dengue virus.
Jung JI, Han SR, Lee SW
Biotechnology letters (2018) 402: 315-324. 201 011 WB

PABP/purine-rich motif as an initiation module for cap-independent translation in pattern-triggered immunity.
Wang J, Zhang X, Greene GH, Xu G, Dong X
Cell (2022) : . 201 011 IP

CD47 interactions with exportin-1 limit the targeting of m7G-modified RNAs to extracellular vesicles.
Kaur S, Saldana AC, Elkahloun AG, Petersen JD, Arakelyan A, Singh SP, Jenkins LM, Kuo B, Reginauld B, Jordan DG, Tran AD, et al.
Journal of cell communication and signaling (2021) : . 201 011 IP; tested species: human

The NSL complex-mediated nucleosome landscape is required to maintain transcription fidelity and suppression of transcription noise.
Lam KC, Chung HR, Semplicio G, Iyer SS, Gaub A, Bhardwaj V, Holz H, Georgiev P, Akhtar A
Genes & development (2019) 337-8: 452-465. 201 011 IP; tested species: drosophila

Cat. No.: 201 011

Amount: 100 µg

Price: $415.00

Capping pores of alphavirus nsP1 gate membranous viral replication factories.
Jones R, Bragagnolo G, Arranz R, Reguera J
Nature (2020) : . 201 011 WB

Development of RNA aptamer that inhibits methyltransferase activity of dengue virus.
Jung JI, Han SR, Lee SW
Biotechnology letters (2018) 402: 315-324. 201 011 WB

PABP/purine-rich motif as an initiation module for cap-independent translation in pattern-triggered immunity.
Wang J, Zhang X, Greene GH, Xu G, Dong X
Cell (2022) : . 201 011 IP

Background

Polymerase II transcripts contain a 5´-terminal m⁷G-cap that is required for the export of these transcripts from the nucleus to the cytoplasm and eucaryotic translation initiation. The Polymerase II transcribed spliceosomal snRNAs U1, U2, U4 and U5 assemble with the eight Sm proteins B/B´, D1, D2, D3, E, F, and G thus forming a core-UsnRNP. The core-UsnRNP is recognized by a methyltransferase that introduces two additional methyl groups to the m⁷G-cap thus forming the m₃G-cap (hypermethylation). The m₃G-cap forms one part of the bipartite nuclear localisation signal (NLS) of the UsnRNPs. It is thus necessary for the nuclear re-import of the core-UsnRNPs. Also certain snoRNAs that are involved in the processing of pre-rRNAs contain an m₃G-cap.

1 ) Bochnig P et al., Eur. J. Biochem. (1987)
2 ) Reuter R et al., Exp. Cell Res. (1984)
3 ) Luhrmann R et al., Nucleic Acids Res. (1982)
4 ) Koziol MJ et al., Bio Protoc (2016)
5 ) Li C et al., J. Virol. (2015)
6 ) Moon SL et al., PLoS Pathog. (2015)
7 ) Dhir A et al., Nat. Struct. Mol. Biol. (2015)
8 ) Shi L et al., J. Immunol. (2014)
9 ) Galipon J et al., Genes Cells (2013)
10 ) Fustin JM et al., Cell (2013)

Protocols

m3G-cap

ICC

ELISA

Standard protocol

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m3G-cap, m7G-cap antibody - 201 011

References for m3G-cap, m7G-cap - 201 011