Synaptic Systems - m3G-cap, m7G-cap

Cat. No.: 201 001

Amount: 100 µl

Price: $355.00

Cat. No. 201 001	100 µl ascites, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: yes ICC: yes (see reference) IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	H20
Subtype	IgG1 (κ light chain)
Immunogen	Synthetic m₃G-cap conjugated to human serum albumin.
Reactivity	Reacts with: human, rat, mouse, eukaryotes. Other species not tested yet.
Specificity	Recognizes m₃G-cap and m⁷G-cap.
Remarks	This antibody can be used to detect capped RNAs (e.g. in viruses) or to identify and purify proteins associated with capped RNAs (see reference #2).
Data sheet	201_001.pdf

References for m3G-cap, m7G-cap - 201 001

mRNA Capping by Venezuelan Equine Encephalitis Virus nsP1: Functional Characterization and Implications for Antiviral Research.
Li C, Guillén J, Rabah N, Blanjoie A, Debart F, Vasseur JJ, Canard B, Decroly E, Coutard B
Journal of virology (2015) 8916: 8292-303. 201 001 WB

MAPCap allows high-resolution detection and differential expression analysis of transcription start sites.
Bhardwaj V, Semplicio G, Erdogdu NU, Manke T, Akhtar A
Nature communications (2019) 101: 3219. 201 001 IP; tested species: drosophila

XRN1 stalling in the 5' UTR of Hepatitis C virus and Bovine Viral Diarrhea virus is associated with dysregulated host mRNA stability.
Moon SL, Blackinton JG, Anderson JR, Dozier MK, Dodd BJ, Keene JD, Wilusz CJ, Bradrick SS, Wilusz J
PLoS pathogens (2015) 113: e1004708. 201 001 IP

Microprocessor mediates transcriptional termination of long noncoding RNA transcripts hosting microRNAs.
Dhir A, Dhir S, Proudfoot NJ, Jopling CL
Nature structural & molecular biology (2015) 224: 319-27. 201 001 IP

Noncoding RNAs and LRRFIP1 regulate TNF expression.
Shi L, Song L, Fitzgerald M, Maurer K, Bagashev A, Sullivan KE
Journal of immunology (Baltimore, Md. : 1950) (2014) 1927: 3057-67. 201 001 IP; tested species: human

RNA-methylation-dependent RNA processing controls the speed of the circadian clock.
Fustin JM, Doi M, Yamaguchi Y, Hida H, Nishimura S, Yoshida M, Isagawa T, Morioka MS, Kakeya H, Manabe I, Okamura H, et al.
Cell (2013) 1554: 793-806. 201 001 IP

Stress-induced lncRNAs evade nuclear degradation and enter the translational machinery.
Galipon J, Miki A, Oda A, Inada T, Ohta K
Genes to cells : devoted to molecular & cellular mechanisms (2013) 185: 353-68. 201 001 IP; tested species: fission yeast

The eIF4E-binding protein Eap1p functions in Vts1p-mediated transcript decay.
Rendl LM, Bieman MA, Vari HK, Smibert CA
PloS one (2012) 710: e47121. 201 001 IP

Identification of a cytoplasmic complex that adds a cap onto 5'-monophosphate RNA.
Otsuka Y, Kedersha NL, Schoenberg DR
Molecular and cellular biology (2009) 298: 2155-67. 201 001 IP

Induced ncRNAs allosterically modify RNA-binding proteins in cis to inhibit transcription.
Wang X, Arai S, Song X, Reichart D, Du K, Pascual G, Tempst P, Rosenfeld MG, Glass CK, Kurokawa R
Nature (2008) 4547200: 126-30. 201 001 IP

The interaction between cap-binding complex and RNA export factor is required for intronless mRNA export.
Nojima T, Hirose T, Kimura H, Hagiwara M
The Journal of biological chemistry (2007) 28221: 15645-51. 201 001 IP

The imprinted Air ncRNA is an atypical RNAPII transcript that evades splicing and escapes nuclear export.
Seidl CI, Stricker SH, Barlow DP
The EMBO journal (2006) 2515: 3565-75. 201 001 IP

Snurportin1, an m3G-cap-specific nuclear import receptor with a novel domain structure.
Huber J, Cronshagen U, Kadokura M, Marshallsay C, Wada T, Sekine M, Lührmann R
The EMBO journal (1998) 1714: 4114-26. 201 001 IP

A monoclonal antibody against 2,2,7-trimethylguanosine that reacts with intact, class U, small nuclear ribonucleoproteins as well as with 7-methylguanosine-capped RNAs.
Bochnig P, Reuter R, Bringmann P, Lührmann R
European journal of biochemistry (1987) 1682: 461-7. 201 001 IP

Cat. No.: 201 001

Amount: 100 µl

Price: $355.00

The eIF4E-binding protein Eap1p functions in Vts1p-mediated transcript decay.
Rendl LM, Bieman MA, Vari HK, Smibert CA
PloS one (2012) 710: e47121. 201 001 IP

Identification of a cytoplasmic complex that adds a cap onto 5'-monophosphate RNA.
Otsuka Y, Kedersha NL, Schoenberg DR
Molecular and cellular biology (2009) 298: 2155-67. 201 001 IP

The imprinted Air ncRNA is an atypical RNAPII transcript that evades splicing and escapes nuclear export.
Seidl CI, Stricker SH, Barlow DP
The EMBO journal (2006) 2515: 3565-75. 201 001 IP

Background

Polymerase II transcripts contain a 5´-terminal m⁷G-cap that is required for the export of these transcripts from the nucleus to the cytoplasm and eucaryotic translation initiation. The Polymerase II transcribed spliceosomal snRNAs U1, U2, U4 and U5 assemble with the eight Sm proteins B/B´, D1, D2, D3, E, F, and G thus forming a core-UsnRNP. The core-UsnRNP is recognized by a methyltransferase that introduces two additional methyl groups to the m⁷G-cap thus forming the m₃G-cap (hypermethylation). The m₃G-cap forms one part of the bipartite nuclear localisation signal (NLS) of the UsnRNPs. It is thus necessary for the nuclear re-import of the core-UsnRNPs. Also certain snoRNAs that are involved in the processing of pre-rRNAs contain an m₃G-cap.

1 ) Bochnig P et al., Eur. J. Biochem. (1987)
2 ) Reuter R et al., Exp. Cell Res. (1984)
3 ) Luhrmann R et al., Nucleic Acids Res. (1982)
4 ) Koziol MJ et al., Bio Protoc (2016)
5 ) Li C et al., J. Virol. (2015)
6 ) Moon SL et al., PLoS Pathog. (2015)
7 ) Dhir A et al., Nat. Struct. Mol. Biol. (2015)
8 ) Shi L et al., J. Immunol. (2014)
9 ) Galipon J et al., Genes Cells (2013)
10 ) Fustin JM et al., Cell (2013)

Protocols

m3G-cap

ICC

Other products for this target

m3G-cap, m7G-cap antibody - 201 001

References for m3G-cap, m7G-cap - 201 001