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Home > Products > 198 211

GAD1 (GAD67) antibody - 198 211

GAD1 and 2 are involved in the synthesis of GABA, the major inhibitory neurotransmitter
Mouse monoclonal purified IgG
Cat. No.: 198 211
Amount: 100 µg
Price: $420.00
Cat. No. 198 211 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500      gallery  
IHC-P: 1 : 1000      gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone 126G12
Subtype IgG2b (κ light chain)
Immunogen Recombinant protein corresponding to residues near the amino-terminus of mouse GAD1. (UniProt Id: P48318)
Epitop AA 94 to 101 from mouse GAD1 (UniProt Id: P48318)
Reactivity Reacts with: mouse (P48318), rat (P18088).
Other species not tested yet.
Specificity Specific for GAD 1 / GAD67.
Data sheet 198_211.pdf

References for GAD1 - 198 211

Differential regulations of neural activity and survival in primary cortical neurons by PFOA or PFHpA.
Ko MY, Park H, Chon SH, Kim YB, Cha SW, Lee BS, Hyun SA, Ka M
Chemosphere (2024) 352: 141379. 198 211 WB; tested species: mouse
ASCL1- and DLX2-induced GABAergic neurons from hiPSC-derived NPCs.
Barretto N, Zhang H, Powell SK, Fernando MB, Zhang S, Flaherty EK, Ho SM, Slesinger PA, Duan J, Brennand KJ
Journal of neuroscience methods (2020) 334: 108548. 198 211 ICC; tested species: human
Dendritic Inhibition by Shh Signaling-Dependent Stellate Cell Pool Is Critical for Motor Learning.
Li W, Chen L, Fleming JT, Brignola E, Zavalin K, Lagrange A, Rex T, Heiney SA, Wojaczynski GJ, Medina JF, Chiang C, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) 4226: 5130-5143. 198 211 IHC; tested species: mouse
Atlastin-1 modulates seizure activity and neuronal excitability.
Lu X, Yang M, Yang Y, Wang XF
CNS neuroscience & therapeutics (2019) : . 198 211 IHC; tested species: human,mouse
Cat. No.: 198 211
Amount: 100 µg
Price: $420.00
Differential regulations of neural activity and survival in primary cortical neurons by PFOA or PFHpA.
Ko MY, Park H, Chon SH, Kim YB, Cha SW, Lee BS, Hyun SA, Ka M
Chemosphere (2024) 352: 141379. 198 211 WB; tested species: mouse
ASCL1- and DLX2-induced GABAergic neurons from hiPSC-derived NPCs.
Barretto N, Zhang H, Powell SK, Fernando MB, Zhang S, Flaherty EK, Ho SM, Slesinger PA, Duan J, Brennand KJ
Journal of neuroscience methods (2020) 334: 108548. 198 211 ICC; tested species: human
Dendritic Inhibition by Shh Signaling-Dependent Stellate Cell Pool Is Critical for Motor Learning.
Li W, Chen L, Fleming JT, Brignola E, Zavalin K, Lagrange A, Rex T, Heiney SA, Wojaczynski GJ, Medina JF, Chiang C, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) 4226: 5130-5143. 198 211 IHC; tested species: mouse
Atlastin-1 modulates seizure activity and neuronal excitability.
Lu X, Yang M, Yang Y, Wang XF
CNS neuroscience & therapeutics (2019) : . 198 211 IHC; tested species: human,mouse
WB
ICC
ICC
IHC
IHC
IHC-P
IHC-P
Background

The glutamic acid decarboxylases GAD1, also referred to as GAD67, and GAD2 / GAD65 synthesize γ-aminobutyric acid (GABA), the major inhibitory neurotransmitter in the central nervous system. The hydrophilic GAD1 can heterodimerize with the membrane achored GAD2 and part of GAD1 is targeted to inhibitory nerve terminals by this mechanisms. Although both proteins exhibit significant differences in their N-terminus they share high homology in the rest of the molecule.
GADs are widely used markers for the GABAergic system. In type 1 diabetes GAD1 has been identified as a major autoantigen.
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