mGluR2 antibody - 191 103

mGluRs are metabotropic glutamate receptors involved in excitatory neurotransmission

Rabbit polyclonal purified antibody

Cat. No.: 191 103

Amount: 50 µg

Price: $380.00

Cat. No. 191 103	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 837 to 853 from rat mGluR2 (UniProt Id: P31421)
Reactivity	Reacts with: rat (P31421), mouse (Q14BI2). Other species not tested yet.
Matching control protein/peptide	191-1P
Data sheet	191_103.pdf

References for mGluR2 - 191 103

INSIHGT: an accessible multi-scale, multi-modal 3D spatial biology platform.
Yau CN, Hung JTS, Campbell RAA, Wong TCY, Huang B, Wong BTY, Chow NKN, Zhang L, Tsoi EPL, Tan Y, Li JJX, et al.
Nature communications (2024) 151: 10888. 191 103 IHC; tested species: mouse

Cat. No.: 191 103

Amount: 50 µg

Price: $380.00

Background

Glutamate is the main excitatory neurotransmitter in the vertebrate nervous system and regulates a number of cellular signaling pathways. In addition to the ionotropic glutamate receptors of the NMDA, AMPA and kainate type, eight metabotropic receptors (mGluR1 - 8) have been described so far (1). This receptor family can be subdivided into three groups (2): Group I receptors (mGluR1, mGluR5) are activated by dihydroxyphenylglycine (DHPG) (3), group II receptors (mGluR2, mGluR3) by dicarboxycyclopropylglycine (DCG-IV), and group III receptors (mGluR4, mGluR6, mGluR7, mGluR8) by aminophosphonobutyrate (L-AP4) (4).

1 ) Shigemoto R et al., J Neurosci (1997)
2 ) Nakanishi S et al., Annu Rev Biophys Biomol Struct (1994)
3 ) Schoepp DD et al., J Neurochem (1994)
4 ) Hayashi Y et al., Nature (1993)

Protocols

OTHER

Preabsorbtion

Other products for this target