Synaptic Systems - Densin180

Cat. No.: 189 003

Amount: 50 µg

Price: $455.00

Ca(V)1 and Ca(V)2 channels engage distinct modes of Ca(2+) signaling to control CREB-dependent gene expression.
Wheeler DG, Groth RD, Ma H, Barrett CF, Owen SF, Safa P, Tsien RW
Cell (2012) 1495: 1112-24. 189 003 ICC; tested species: rat

ICC

Background

Densin is a 180 kDa transmembrane glycoprotein which belongs to the LAP family [leucine-rich repeat (LRR) and PDZ] family, characterized by an LRR near the N-terminus and one or more PDZ domains at the C-terminus. Several developmentally regulated splice variants and protein-protein interactions with CamKII and nephrin have been described. The protein locates to the post-synaptic density (PSD) in nerve cells but has also been shown to be expressed in glomerular podocytes in kidney.

1 ) Ahola H et al., J. Am. Soc. Nephrol. (2003)
2 ) Walikonis RS et al., J. Neurosci. (2001)
3 ) Strack S et al., J. Biol. Chem. (2000)
4 ) Apperson ML et al., J. Neurosci. (1996)

Protocols

ICC

Other products for this target

Cat. No. 189 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: yes (see remarks) IP: not tested yet ICC: 1 : 500 gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 877 to 891 from rat Densin 180 (UniProt Id: P70587)
Reactivity	Reacts with: rat (P70587), mouse (Q80TE7). Other species not tested yet.
Remarks	WB: The crude antiserum (cat. no. 189 002) is recommended for WB (stronger signals).
Data sheet	189_003.pdf

Densin180 antibody - 189 003

References for Densin180 - 189 003