MAP2 antibody - 188 111

MAP2 is a major microtubule associated protein in neurons

Mouse monoclonal purified IgG

Cat. No.: 188 111

Amount: 100 µg

Price: $420.00

Cat. No. 188 111	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery IP: not tested yet ICC: 1 : 1000 gallery IHC: 1 : 500 up to 1 : 1000 gallery IHC-P: 1 : 1000 up to 1 : 2000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	SY-230A4
Subtype	IgG2b (κ light chain)
Immunogen	Recombinant protein corresponding to residues near the amino terminus of human Map2 (UniProt Id: P11137-4)
Reactivity	Reacts with: mouse (P20357), rat (P15146), human (P11137-4). Other species not tested yet.
Matching control protein/peptide	188-0P
Remarks	WB: To avoid protein aggregation, do not heat samples for SDS-PAGE. Due to the large size of this protein, we recommend NuPAGE 3-8% Tris-Acetate gels for SDS-PAGE.
Data sheet	188_111.pdf

Cat. No.: 188 111

Amount: 100 µg

Price: $420.00

ICC

IHC

IHC-P

Background

There are two major classes of heat stable microtubule associated proteins (MAPs): MAP2, and tau. Both protein classes are involved in the regulation of microtubule polymerization in cells.
Four differentially regulated isoforms of MAP2 have been described so far.

1 ) Dehmelt L et al., Genome Biol. (2005)
2 ) Al-Bassam J et al., J. Cell Biol. (2002)
3 ) Di Stefano G et al., J. Histochem. Cytochem. (2001)
4 ) Chauhan N et al., Neurochem. Res. (1997)
5 ) Sánchez C et al., Biochem. J. (1995)
6 ) Izant JG et al., Proc. Natl. Acad. Sci. U.S.A. (1980)

Protocols

ICC

IHC

IHC-P

OTHER

Preabsorbtion

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