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GluA1 antibody - 182 003 K.O.

GluAs are ionotropic glutamate receptors involved in rapid excitatory neurotransmission
Rabbit polyclonal purified antibody
Cat. No.: 182 003
Amount: 50 µg
Price: $380.00
Cat. No. 182 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 (see remarks) gallery  
IHC: not tested yet
IHC-P: not tested yet
ExM: yes

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Expansion Microscopy (ExM) is a sample preparation and imaging method which employs a dense interconnected web of swellable polymer within a biological specimen. A significantly higher effective resolution can be achieved with a standard microscope setup. For additional experimental details, please refer to the Remarks section.
Immunogen Recombinant protein corresponding to AA 829 to 907 from mouse GluA1 (UniProt Id: P23818)
Reactivity Reacts with: human (P42261), rat (P19490), mouse (P23818).
No signal: zebrafish.
Other species not tested yet.
Specificity K.O. validated PubMed: 32831170
Matching control protein/peptide 182-0P
Remarks

ICC: Methanol fixation is recommended.
Data sheet 182_003.pdf

References for GluA1 - 182 003

Synaptic neoteny of human cortical neurons requires species-specific balancing of SRGAP2-SYNGAP1 cross-inhibition.
Libé-Philippot B, Iwata R, Recupero AJ, Wierda K, Bernal Garcia S, Hammond L, van Benthem A, Limame R, Ditkowska M, Beckers S, Gaspariunaite V, et al.
Neuron (2024) : . 182 003 WB; tested species: human
SNX17 Mediates Dendritic Spine Maturation via p140Cap.
Cui Q, Liang S, Li H, Guo Y, Lv J, Wang X, Qin P, Xu H, Huang TY, Lu Y, Tian Q, et al.
Molecular neurobiology (2023) : . 182 003 WB; tested species: mouse
Neurons undergo pathogenic metabolic reprogramming in models of familial ALS.
Riechers SP, Mojsilovic-Petrovic J, Belton TB, Chakrabarty RP, Garjani M, Medvedeva V, Dalton C, Wong YC, Chandel NS, Dienel G, Kalb RG, et al.
Molecular metabolism (2022) 60: 101468. 182 003 WB, ICC; tested species: rat
Proteome dynamics during homeostatic scaling in cultured neurons.
Dörrbaum AR, Alvarez-Castelao B, Nassim-Assir B, Langer JD, Schuman EM
eLife (2020) 9: . 182 003 WB; tested species: rat
Long-term potentiation is independent of the C-tail of the GluA1 AMPA receptor subunit.
Díaz-Alonso J, Morishita W, Incontro S, Simms J, Holtzman J, Gill M, Mucke L, Malenka RC, Nicoll RA
eLife (2020) 9: . 182 003 WB, IHC; KO verified; tested species: mouse
LAR receptor phospho-tyrosine phosphatases regulate NMDA-receptor responses.
Sclip A, Südhof TC
eLife (2020) 9: . 182 003 WB; tested species: mouse
Postsynaptic densities fragment into subcomplexes upon sonication.
Dosemeci A, Tao-Cheng JH, Bakly V, Reese TS
Molecular brain (2019) 121: 72. 182 003 WB; tested species: rat
Chronic Toxoplasma infection is associated with distinct alterations in the synaptic protein composition.
Lang D, Schott BH, van Ham M, Morton L, Kulikovskaja L, Herrera-Molina R, Pielot R, Klawonn F, Montag D, Jänsch L, Gundelfinger ED, et al.
Journal of neuroinflammation (2018) 151: 216. 182 003 WB; tested species: mouse
Synaptotagmin-3 drives AMPA receptor endocytosis, depression of synapse strength, and forgetting.
Awasthi A, Ramachandran B, Ahmed S, Benito E, Shinoda Y, Nitzan N, Heukamp A, Rannio S, Martens H, Barth J, Burk K, et al.
Science (New York, N.Y.) (2018) : . 182 003 WB, ICC; tested species: rat
Development and Characterization of Human Cerebral Organoids: An Optimized Protocol.
Yakoub AM, Sadek M
Cell transplantation (2018) 273: 393-406. 182 003 WB; tested species: human
Autism-like behavior caused by deletion of vaccinia-related kinase 3 is improved by TrkB stimulation.
Kang MS, Choi TY, Ryu HG, Lee D, Lee SH, Choi SY, Kim KT
The Journal of experimental medicine (2017) 21410: 2947-2966. 182 003 WB; tested species: mouse
Treatment of intermittent hypoxia increases phosphorylated tau in the hippocampus via biological processes common to aging.
Yagishita S, Suzuki S, Yoshikawa K, Iida K, Hirata A, Suzuki M, Takashima A, Maruyama K, Hirasawa A, Awaji T
Molecular brain (2017) 101: 2. 182 003 WB
Dysfunctional cerebellar Purkinje cells contribute to autism-like behaviour in Shank2-deficient mice.
Peter S, Ten Brinke MM, Stedehouder J, Reinelt CM, Wu B, Zhou H, Zhou K, Boele HJ, Kushner SA, Lee MG, Schmeisser MJ, et al.
Nature communications (2016) 7: 12627. 182 003 WB; tested species: mouse
Mechanisms of Functional Hypoconnectivity in the Medial Prefrontal Cortex of Mecp2 Null Mice.
Sceniak MP, Lang M, Enomoto AC, James Howell C, Hermes DJ, Katz DM
Cerebral cortex (New York, N.Y. : 1991) (2016) 265: 1938-1956. 182 003 WB; tested species: mouse
How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs.
Acuna C, Liu X, Südhof TC
Neuron (2016) 914: 792-807. 182 003 WB
The intellectual disability protein RAB39B selectively regulates GluA2 trafficking to determine synaptic AMPAR composition.
Mignogna ML, Giannandrea M, Gurgone A, Fanelli F, Raimondi F, Mapelli L, Bassani S, Fang H, Van Anken E, Alessio M, Passafaro M, et al.
Nature communications (2015) 6: 6504. 182 003 WB; tested species: mouse
Deletion of olfactomedin 2 induces changes in the AMPA receptor complex and impairs visual, olfactory, and motor functions in mice.
Sultana A, Nakaya N, Dong L, Abu-Asab M, Qian H, Tomarev SI
Experimental neurology (2014) 261: 802-11. 182 003 WB
IκB kinase/nuclear factor κB-dependent insulin-like growth factor 2 (Igf2) expression regulates synapse formation and spine maturation via Igf2 receptor signaling.
Schmeisser MJ, Baumann B, Johannsen S, Vindedal GF, Jensen V, Hvalby ØC, Sprengel R, Seither J, Maqbool A, Magnutzki A, Lattke M, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3216: 5688-703. 182 003 WB; tested species: mouse
Autistic-like behaviours and hyperactivity in mice lacking ProSAP1/Shank2.
Schmeisser MJ, Ey E, Wegener S, Bockmann J, Stempel AV, Kuebler A, Janssen AL, Udvardi PT, Shiban E, Spilker C, Balschun D, et al.
Nature (2012) 4867402: 256-60. 182 003 WB
The anaphase promoting complex is required for memory function in mice.
Kuczera T, Stilling RM, Hsia HE, Bahari-Javan S, Irniger S, Nasmyth K, Sananbenesi F, Fischer A
Learning & memory (Cold Spring Harbor, N.Y.) (2011) 181: 49-57. 182 003 WB
High-resolution proteomics unravel architecture and molecular diversity of native AMPA receptor complexes.
Schwenk J, Harmel N, Brechet A, Zolles G, Berkefeld H, Müller CS, Bildl W, Baehrens D, Hüber B, Kulik A, Klöcker N, et al.
Neuron (2012) 744: 621-33. 182 003 IP; tested species: rat
Neurons undergo pathogenic metabolic reprogramming in models of familial ALS.
Riechers SP, Mojsilovic-Petrovic J, Belton TB, Chakrabarty RP, Garjani M, Medvedeva V, Dalton C, Wong YC, Chandel NS, Dienel G, Kalb RG, et al.
Molecular metabolism (2022) 60: 101468. 182 003 WB, ICC; tested species: rat
Acute knockdown of Depdc5 leads to synaptic defects in mTOR-related epileptogenesis.
De Fusco A, Cerullo MS, Marte A, Michetti C, Romei A, Castroflorio E, Baulac S, Benfenati F
Neurobiology of disease (2020) : 104822. 182 003 ICC; tested species: mouse
Differential Scaling of Synaptic Molecules within Functional Zones of an Excitatory Synapse during Homeostatic Plasticity.
Venkatesan S, Subramaniam S, Rajeev P, Chopra Y, Jose M, Nair D
eNeuro (2020) 72: . 182 003 ICC; tested species: rat
Kibra Modulates Learning and Memory via Binding to Dendrin.
Ji Z, Li H, Yang Z, Huang X, Ke X, Ma S, Lin Z, Lu Y, Zhang M
Cell reports (2019) 268: 2064-2077.e7. 182 003 ICC; tested species: mouse
Synaptotagmin-3 drives AMPA receptor endocytosis, depression of synapse strength, and forgetting.
Awasthi A, Ramachandran B, Ahmed S, Benito E, Shinoda Y, Nitzan N, Heukamp A, Rannio S, Martens H, Barth J, Burk K, et al.
Science (New York, N.Y.) (2018) : . 182 003 WB, ICC; tested species: rat
Latrophilin-2 and latrophilin-3 are redundantly essential for parallel-fiber synapse function in cerebellum.
Zhang RS, Liakath-Ali K, Südhof TC
eLife (2020) 9: . 182 003 IHC; tested species: mouse
Long-term potentiation is independent of the C-tail of the GluA1 AMPA receptor subunit.
Díaz-Alonso J, Morishita W, Incontro S, Simms J, Holtzman J, Gill M, Mucke L, Malenka RC, Nicoll RA
eLife (2020) 9: . 182 003 WB, IHC; KO verified; tested species: mouse
Patch2MAP combines patch-clamp electrophysiology with super-resolution structural and protein imaging in identified single neurons without genetic modification.
Vardalaki D, Pham TLD, Frosch MP, Cosgrove GR, Richardson M, Cash SS, Harnett MT
bioRxiv : the preprint server for biology (2023) : . 182 003 ExM; tested species: human
Cat. No.: 182 003
Amount: 50 µg
Price: $380.00
Synaptic neoteny of human cortical neurons requires species-specific balancing of SRGAP2-SYNGAP1 cross-inhibition.
Libé-Philippot B, Iwata R, Recupero AJ, Wierda K, Bernal Garcia S, Hammond L, van Benthem A, Limame R, Ditkowska M, Beckers S, Gaspariunaite V, et al.
Neuron (2024) : . 182 003 WB; tested species: human
SNX17 Mediates Dendritic Spine Maturation via p140Cap.
Cui Q, Liang S, Li H, Guo Y, Lv J, Wang X, Qin P, Xu H, Huang TY, Lu Y, Tian Q, et al.
Molecular neurobiology (2023) : . 182 003 WB; tested species: mouse
Neurons undergo pathogenic metabolic reprogramming in models of familial ALS.
Riechers SP, Mojsilovic-Petrovic J, Belton TB, Chakrabarty RP, Garjani M, Medvedeva V, Dalton C, Wong YC, Chandel NS, Dienel G, Kalb RG, et al.
Molecular metabolism (2022) 60: 101468. 182 003 WB, ICC; tested species: rat
Proteome dynamics during homeostatic scaling in cultured neurons.
Dörrbaum AR, Alvarez-Castelao B, Nassim-Assir B, Langer JD, Schuman EM
eLife (2020) 9: . 182 003 WB; tested species: rat
Long-term potentiation is independent of the C-tail of the GluA1 AMPA receptor subunit.
Díaz-Alonso J, Morishita W, Incontro S, Simms J, Holtzman J, Gill M, Mucke L, Malenka RC, Nicoll RA
eLife (2020) 9: . 182 003 WB, IHC; KO verified; tested species: mouse
LAR receptor phospho-tyrosine phosphatases regulate NMDA-receptor responses.
Sclip A, Südhof TC
eLife (2020) 9: . 182 003 WB; tested species: mouse
Postsynaptic densities fragment into subcomplexes upon sonication.
Dosemeci A, Tao-Cheng JH, Bakly V, Reese TS
Molecular brain (2019) 121: 72. 182 003 WB; tested species: rat
Chronic Toxoplasma infection is associated with distinct alterations in the synaptic protein composition.
Lang D, Schott BH, van Ham M, Morton L, Kulikovskaja L, Herrera-Molina R, Pielot R, Klawonn F, Montag D, Jänsch L, Gundelfinger ED, et al.
Journal of neuroinflammation (2018) 151: 216. 182 003 WB; tested species: mouse
Synaptotagmin-3 drives AMPA receptor endocytosis, depression of synapse strength, and forgetting.
Awasthi A, Ramachandran B, Ahmed S, Benito E, Shinoda Y, Nitzan N, Heukamp A, Rannio S, Martens H, Barth J, Burk K, et al.
Science (New York, N.Y.) (2018) : . 182 003 WB, ICC; tested species: rat
Development and Characterization of Human Cerebral Organoids: An Optimized Protocol.
Yakoub AM, Sadek M
Cell transplantation (2018) 273: 393-406. 182 003 WB; tested species: human
Autism-like behavior caused by deletion of vaccinia-related kinase 3 is improved by TrkB stimulation.
Kang MS, Choi TY, Ryu HG, Lee D, Lee SH, Choi SY, Kim KT
The Journal of experimental medicine (2017) 21410: 2947-2966. 182 003 WB; tested species: mouse
Treatment of intermittent hypoxia increases phosphorylated tau in the hippocampus via biological processes common to aging.
Yagishita S, Suzuki S, Yoshikawa K, Iida K, Hirata A, Suzuki M, Takashima A, Maruyama K, Hirasawa A, Awaji T
Molecular brain (2017) 101: 2. 182 003 WB
Dysfunctional cerebellar Purkinje cells contribute to autism-like behaviour in Shank2-deficient mice.
Peter S, Ten Brinke MM, Stedehouder J, Reinelt CM, Wu B, Zhou H, Zhou K, Boele HJ, Kushner SA, Lee MG, Schmeisser MJ, et al.
Nature communications (2016) 7: 12627. 182 003 WB; tested species: mouse
Mechanisms of Functional Hypoconnectivity in the Medial Prefrontal Cortex of Mecp2 Null Mice.
Sceniak MP, Lang M, Enomoto AC, James Howell C, Hermes DJ, Katz DM
Cerebral cortex (New York, N.Y. : 1991) (2016) 265: 1938-1956. 182 003 WB; tested species: mouse
How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs.
Acuna C, Liu X, Südhof TC
Neuron (2016) 914: 792-807. 182 003 WB
The intellectual disability protein RAB39B selectively regulates GluA2 trafficking to determine synaptic AMPAR composition.
Mignogna ML, Giannandrea M, Gurgone A, Fanelli F, Raimondi F, Mapelli L, Bassani S, Fang H, Van Anken E, Alessio M, Passafaro M, et al.
Nature communications (2015) 6: 6504. 182 003 WB; tested species: mouse
Deletion of olfactomedin 2 induces changes in the AMPA receptor complex and impairs visual, olfactory, and motor functions in mice.
Sultana A, Nakaya N, Dong L, Abu-Asab M, Qian H, Tomarev SI
Experimental neurology (2014) 261: 802-11. 182 003 WB
IκB kinase/nuclear factor κB-dependent insulin-like growth factor 2 (Igf2) expression regulates synapse formation and spine maturation via Igf2 receptor signaling.
Schmeisser MJ, Baumann B, Johannsen S, Vindedal GF, Jensen V, Hvalby ØC, Sprengel R, Seither J, Maqbool A, Magnutzki A, Lattke M, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3216: 5688-703. 182 003 WB; tested species: mouse
Autistic-like behaviours and hyperactivity in mice lacking ProSAP1/Shank2.
Schmeisser MJ, Ey E, Wegener S, Bockmann J, Stempel AV, Kuebler A, Janssen AL, Udvardi PT, Shiban E, Spilker C, Balschun D, et al.
Nature (2012) 4867402: 256-60. 182 003 WB
The anaphase promoting complex is required for memory function in mice.
Kuczera T, Stilling RM, Hsia HE, Bahari-Javan S, Irniger S, Nasmyth K, Sananbenesi F, Fischer A
Learning & memory (Cold Spring Harbor, N.Y.) (2011) 181: 49-57. 182 003 WB
High-resolution proteomics unravel architecture and molecular diversity of native AMPA receptor complexes.
Schwenk J, Harmel N, Brechet A, Zolles G, Berkefeld H, Müller CS, Bildl W, Baehrens D, Hüber B, Kulik A, Klöcker N, et al.
Neuron (2012) 744: 621-33. 182 003 IP; tested species: rat
Neurons undergo pathogenic metabolic reprogramming in models of familial ALS.
Riechers SP, Mojsilovic-Petrovic J, Belton TB, Chakrabarty RP, Garjani M, Medvedeva V, Dalton C, Wong YC, Chandel NS, Dienel G, Kalb RG, et al.
Molecular metabolism (2022) 60: 101468. 182 003 WB, ICC; tested species: rat
Acute knockdown of Depdc5 leads to synaptic defects in mTOR-related epileptogenesis.
De Fusco A, Cerullo MS, Marte A, Michetti C, Romei A, Castroflorio E, Baulac S, Benfenati F
Neurobiology of disease (2020) : 104822. 182 003 ICC; tested species: mouse
Differential Scaling of Synaptic Molecules within Functional Zones of an Excitatory Synapse during Homeostatic Plasticity.
Venkatesan S, Subramaniam S, Rajeev P, Chopra Y, Jose M, Nair D
eNeuro (2020) 72: . 182 003 ICC; tested species: rat
Kibra Modulates Learning and Memory via Binding to Dendrin.
Ji Z, Li H, Yang Z, Huang X, Ke X, Ma S, Lin Z, Lu Y, Zhang M
Cell reports (2019) 268: 2064-2077.e7. 182 003 ICC; tested species: mouse
Synaptotagmin-3 drives AMPA receptor endocytosis, depression of synapse strength, and forgetting.
Awasthi A, Ramachandran B, Ahmed S, Benito E, Shinoda Y, Nitzan N, Heukamp A, Rannio S, Martens H, Barth J, Burk K, et al.
Science (New York, N.Y.) (2018) : . 182 003 WB, ICC; tested species: rat
Latrophilin-2 and latrophilin-3 are redundantly essential for parallel-fiber synapse function in cerebellum.
Zhang RS, Liakath-Ali K, Südhof TC
eLife (2020) 9: . 182 003 IHC; tested species: mouse
Long-term potentiation is independent of the C-tail of the GluA1 AMPA receptor subunit.
Díaz-Alonso J, Morishita W, Incontro S, Simms J, Holtzman J, Gill M, Mucke L, Malenka RC, Nicoll RA
eLife (2020) 9: . 182 003 WB, IHC; KO verified; tested species: mouse
Patch2MAP combines patch-clamp electrophysiology with super-resolution structural and protein imaging in identified single neurons without genetic modification.
Vardalaki D, Pham TLD, Frosch MP, Cosgrove GR, Richardson M, Cash SS, Harnett MT
bioRxiv : the preprint server for biology (2023) : . 182 003 ExM; tested species: human
WB
WB
ICC
Background

Ionotropic glutamate receptors (iGluRs) mediate rapid excitatory neurotransmission in the mammalian CNS. They can be subdivided into three major groups, the AMPA/GluA, NMDA/GluN and kainate/GluK receptors (KARs). mRNAs coding for glutamate receptors are substrates for an adenosine deaminase acting on RNA (ADAR) that increases the diversity of these proteins.
Glutamate receptors of the AMPA subtype are monovalent cation channels and are composed of the four AMPA subunits GluA 1, GluA 2, GluA 3, and GluA 4.
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