TRIM9 antibody - 181 003

TRIM9 is a SNAP 25 interaction partner

Rabbit polyclonal purified antibody

Cat. No.: 181 003

Amount: 50 µg

Price: $375.00

Cat. No. 181 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 250 from human TRIM9 (UniProt Id: Q9C026)
Reactivity	Reacts with: human (Q9C026), rat (Q91ZY8), mouse (Q8C7M3), cow. Other species not tested yet.
Data sheet	181_003.pdf

Cat. No.: 181 003

Amount: 50 µg

Price: $375.00

Background

TRIM 9, also referred to as Spring, is a member of the tripartite motif protein family. These proteins are composed of three zinc-binding domains, a RING, a B-box type 1, a B-box type 2 and a coiled-coil region. Proteins belonging to this large family are involved in cellular processes ranging from cell-growth and development to the regulation of fusion events necessary for exocytosis.
TRIM 9 is expressed selectively in the adult and embryonic nervous system and has been implicated in the regulation of synaptic vesicle exocytosis via SNAP 25 interaction. Several splice variant with molecular weights from 60 - 80 kDa have been described.

1 ) Short KM et al., J. Biol. Chem. (2006)
2 ) Nisole S et al., Nat. Rev. Microbiol. (2005)
3 ) Berti C et al., Mech. Dev. (2002)
4 ) Reymond A et al., EMBO J. (2001)

Protocols

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