Synaptic Systems

Cat. No.: 180 003

Amount: 50 µg

Price: $380.00

Cat. No. 180 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 (see remarks) gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 844 to 908 from rat GluK2 (UniProt Id: P42260)
Reactivity	Reacts with: human (Q13002), rat (P42260), mouse (P39087). Other species not tested yet.
Specificity	K.O. validated PubMed: 26448475
Matching control protein/peptide	180-0P
Remarks	IHC: For best results, heat-mediated antigen retrieval (citrate buffer pH 6) is recommended.
Data sheet	180_003.pdf

References for GluK2 - 180 003

Behavioral analysis of kainate receptor KO mice and the role of GluK3 subunit in anxiety.
Iida I, Konno K, Natsume R, Abe M, Watanabe M, Sakimura K, Terunuma M
Scientific reports (2024) 141: 4521. 180 003 WB; tested species: mouse

A comparative analysis of kainate receptor GluK2 and GluK5 knockout mice in a pure genetic background.
Iida I, Konno K, Natsume R, Abe M, Watanabe M, Sakimura K, Terunuma M
Behavioural brain research (2021) 405: 113194. 180 003 WB, IHC-Fr; KO verified

Ccny knockout mice display an enhanced susceptibility to kainic acid-induced epilepsy.
Hwang H, Seo J, Choi Y, Cho E, Sohn H, Jang J, Lee AR, Lee J, Kim S, Koh HY, Park M, et al.
Pharmacological research (2020) 160: 105100. 180 003 WB; tested species: mouse

Expression mapping, quantification, and complex formation of GluD1 and GluD2 glutamate receptors in adult mouse brain.
Nakamoto C, Konno K, Miyazaki T, Nakatsukasa E, Natsume R, Abe M, Kawamura M, Fukazawa Y, Shigemto R, Yamasaki M, Sakimura K, et al.
The Journal of comparative neurology (2019) : . 180 003 WB; tested species: mouse

Determination of kainate receptor subunit ratios in mouse brain using novel chimeric protein standards.
Watanabe-Iida I, Konno K, Akashi K, Abe M, Natsume R, Watanabe M, Sakimura K
Journal of neurochemistry (2016) 1362: 295-305. 180 003 WB; KO verified; tested species: mouse

Novel application of stem cell-derived neurons to evaluate the time- and dose-dependent progression of excitotoxic injury.
Gut IM, Beske PH, Hubbard KS, Lyman ME, Hamilton TA, McNutt PM
PloS one (2013) 85: e64423. 180 003 WB

INSIHGT: an accessible multi-scale, multi-modal 3D spatial biology platform.
Yau CN, Hung JTS, Campbell RAA, Wong TCY, Huang B, Wong BTY, Chow NKN, Zhang L, Tsoi EPL, Tan Y, Li JJX, et al.
Nature communications (2024) 151: 10888. 180 003 IHC; tested species: mouse

Presenilin and APP Regulate Synaptic Kainate Receptors.
Barthet G, Moreira-de-Sá A, Zhang P, Deforges S, Castanheira J, Gorlewicz A, Mulle C
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) 4249: 9253-9262. 180 003 IHC-Fr; tested species: mouse

Cat. No.: 180 003

Amount: 50 µg

Price: $380.00

ICC

IHC

Background

Ionotropic glutamate receptors (iGluRs) mediate rapid excitatory neurotransmission in the mammalian CNS. They can be subdivided into three major groups, the AMPA/GluA, NMDA/GluN, and kainate/GluK receptors (KARs).
mRNAs coding for glutamate receptors are substrates for an adenosine deaminase acting on RNA (ADAR) that increases the diversity of these proteins. KARs can be found at pre- and postsynaptic sites and are composed of five different subunits: GluK1, GluK2 and GluK3 can form homomeric receptors whereas GluK4 and GluK5 form heteromeric receptors with GluK1-3.

1 ) Traynelis SF et al., Pharmacol Rev (2010)
2 ) Collingridge GL et al., Neuropharmacology (2009)
3 ) Bass BL et al., RNA (1997)
4 ) Kumar J et al., Neuron (2011)
5 ) Pinheiro PS et al., Proc. Natl. Acad. Sci. U.S.A. (2007)
6 ) Jaskolski F et al., J. Biol. Chem. (2005)
7 ) Hamassaki-Britto DE et al., J. Neurosci. (1993)
8 ) Strutz-Seebohm N et al., Cell. Physiol. Biochem. (2006)
9 ) Coussen F et al., Neuron (2005)
10 ) Ullal G et al., Epilepsia (2005)

Protocols

ICC

IHC

OTHER

Preabsorbtion

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GluK2 antibody - 180 003 K.O.

References for GluK2 - 180 003