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Home > Products > 176 003

Myobrevin antibody - 176 003 K.O.

Myobrevin or Vamp5 is a VAMP isoform expressed preferentially in skeletal muscle and heart
Rabbit polyclonal purified antibody
Cat. No.: 176 003
Amount: 50 µg
Price: $375.00
Cat. No. 176 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 1000 gallery  
IHC: yes
IHC-P: 1 : 200 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to AA 1 to 70 from mouse Myobrevin (UniProt Id: Q9Z2P8)
Reactivity Reacts with: mouse (Q9Z2P8).
No signal: rat.
Other species not tested yet.
Specificity K.O. validated PubMed: 29330887
Matching control protein/peptide 176-0P
Data sheet 176_003.pdf

References for Myobrevin - 176 003

Lysosomal exocytosis releases pathogenic α-synuclein species from neurons in synucleinopathy models.
Xie YX, Naseri NN, Fels J, Kharel P, Na Y, Lane D, Burré J, Sharma M
Nature communications (2022) 131: 4918. 176 003 WB; tested species: mouse
Characterisation of GLUT4 trafficking in HeLa cells: comparable kinetics and orthologous trafficking mechanisms to 3T3-L1 adipocytes.
Morris S, Geoghegan ND, Sadler JBA, Koester AM, Black HL, Laub M, Miller L, Heffernan L, Simpson JC, Mastick CC, Cooper J, et al.
PeerJ (2020) 8: e8751. 176 003 WB; tested species: mouse
Cardiac SNARE Expression in Health and Disease.
Bowman PRT, Smith GL, Gould GW
Frontiers in endocrinology (2019) 10: 881. 176 003 WB; tested species: mouse
Loss of VAMP5 in mice results in duplication of the ureter and insufficient expansion of the lung.
Ikezawa M, Tajika Y, Ueno H, Murakami T, Inoue N, Yorifuji H
Developmental dynamics : an official publication of the American Association of Anatomists (2018) : . 176 003 WB, IHC; KO verified; tested species: mouse
Characterization of VAMP isoforms in 3T3-L1 adipocytes: implications for GLUT4 trafficking.
Sadler JB, Bryant NJ, Gould GW
Molecular biology of the cell (2015) 263: 530-6. 176 003 WB
Vesicular transport system in myotubes: ultrastructural study and signposting with vesicle-associated membrane proteins.
Tajika Y, Takahashi M, Khairani AF, Ueno H, Murakami T, Yorifuji H
Histochemistry and cell biology (2014) 1414: 441-54. 176 003 WB, ICC; tested species: mouse
The localization of VAMP5 in skeletal and cardiac muscle.
Takahashi M, Tajika Y, Khairani AF, Ueno H, Murakami T, Yorifuji H
Histochemistry and cell biology (2013) 1394: 573-82. 176 003 WB, IHC
Effects of contraction on localization of GLUT4 and v-SNARE isoforms in rat skeletal muscle.
Rose AJ, Jeppesen J, Kiens B, Richter EA
American journal of physiology. Regulatory, integrative and comparative physiology (2009) 2975: R1228-37. 176 003 WB
SNAP23 deficiency causes severe brain dysplasia through the loss of radial glial cell polarity.
Kunii M, Noguchi Y, Yoshimura SI, Kanda S, Iwano T, Avriyanti E, Atik N, Sato T, Sato K, Ogawa M, Harada A, et al.
The Journal of cell biology (2021) 2201: . 176 003 ICC; KD verified; tested species: mouse
Vesicular transport system in myotubes: ultrastructural study and signposting with vesicle-associated membrane proteins.
Tajika Y, Takahashi M, Khairani AF, Ueno H, Murakami T, Yorifuji H
Histochemistry and cell biology (2014) 1414: 441-54. 176 003 WB, ICC; tested species: mouse
Loss of VAMP5 in mice results in duplication of the ureter and insufficient expansion of the lung.
Ikezawa M, Tajika Y, Ueno H, Murakami T, Inoue N, Yorifuji H
Developmental dynamics : an official publication of the American Association of Anatomists (2018) : . 176 003 WB, IHC; KO verified; tested species: mouse
The localization of VAMP5 in skeletal and cardiac muscle.
Takahashi M, Tajika Y, Khairani AF, Ueno H, Murakami T, Yorifuji H
Histochemistry and cell biology (2013) 1394: 573-82. 176 003 WB, IHC
Cat. No.: 176 003
Amount: 50 µg
Price: $375.00
Lysosomal exocytosis releases pathogenic α-synuclein species from neurons in synucleinopathy models.
Xie YX, Naseri NN, Fels J, Kharel P, Na Y, Lane D, Burré J, Sharma M
Nature communications (2022) 131: 4918. 176 003 WB; tested species: mouse
Characterisation of GLUT4 trafficking in HeLa cells: comparable kinetics and orthologous trafficking mechanisms to 3T3-L1 adipocytes.
Morris S, Geoghegan ND, Sadler JBA, Koester AM, Black HL, Laub M, Miller L, Heffernan L, Simpson JC, Mastick CC, Cooper J, et al.
PeerJ (2020) 8: e8751. 176 003 WB; tested species: mouse
Cardiac SNARE Expression in Health and Disease.
Bowman PRT, Smith GL, Gould GW
Frontiers in endocrinology (2019) 10: 881. 176 003 WB; tested species: mouse
Loss of VAMP5 in mice results in duplication of the ureter and insufficient expansion of the lung.
Ikezawa M, Tajika Y, Ueno H, Murakami T, Inoue N, Yorifuji H
Developmental dynamics : an official publication of the American Association of Anatomists (2018) : . 176 003 WB, IHC; KO verified; tested species: mouse
Characterization of VAMP isoforms in 3T3-L1 adipocytes: implications for GLUT4 trafficking.
Sadler JB, Bryant NJ, Gould GW
Molecular biology of the cell (2015) 263: 530-6. 176 003 WB
Vesicular transport system in myotubes: ultrastructural study and signposting with vesicle-associated membrane proteins.
Tajika Y, Takahashi M, Khairani AF, Ueno H, Murakami T, Yorifuji H
Histochemistry and cell biology (2014) 1414: 441-54. 176 003 WB, ICC; tested species: mouse
The localization of VAMP5 in skeletal and cardiac muscle.
Takahashi M, Tajika Y, Khairani AF, Ueno H, Murakami T, Yorifuji H
Histochemistry and cell biology (2013) 1394: 573-82. 176 003 WB, IHC
Effects of contraction on localization of GLUT4 and v-SNARE isoforms in rat skeletal muscle.
Rose AJ, Jeppesen J, Kiens B, Richter EA
American journal of physiology. Regulatory, integrative and comparative physiology (2009) 2975: R1228-37. 176 003 WB
SNAP23 deficiency causes severe brain dysplasia through the loss of radial glial cell polarity.
Kunii M, Noguchi Y, Yoshimura SI, Kanda S, Iwano T, Avriyanti E, Atik N, Sato T, Sato K, Ogawa M, Harada A, et al.
The Journal of cell biology (2021) 2201: . 176 003 ICC; KD verified; tested species: mouse
Vesicular transport system in myotubes: ultrastructural study and signposting with vesicle-associated membrane proteins.
Tajika Y, Takahashi M, Khairani AF, Ueno H, Murakami T, Yorifuji H
Histochemistry and cell biology (2014) 1414: 441-54. 176 003 WB, ICC; tested species: mouse
Loss of VAMP5 in mice results in duplication of the ureter and insufficient expansion of the lung.
Ikezawa M, Tajika Y, Ueno H, Murakami T, Inoue N, Yorifuji H
Developmental dynamics : an official publication of the American Association of Anatomists (2018) : . 176 003 WB, IHC; KO verified; tested species: mouse
The localization of VAMP5 in skeletal and cardiac muscle.
Takahashi M, Tajika Y, Khairani AF, Ueno H, Murakami T, Yorifuji H
Histochemistry and cell biology (2013) 1394: 573-82. 176 003 WB, IHC
WB
ICC
IHC-P
Background
Myobrevin, also known as VAMP 5 belongs to the family of vesicle-associated membrane proteins and has a theoretical molecular weight of 11.4 kDa. Like other VAMP isoforms it is composed of an N-terminal cytoplasmic region and a C-terminal transmembrane domain.
Vamp 5 is preferentially expressed in skeletal muscle and heart tissue and is upregulated during the differentiation of C2C12 cells into myotubes.
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