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Neurofilament M antibody - 171 204

Neurofilaments are major neuronal intermediate filaments
Guinea pig polyclonal antiserum
Cat. No.: 171 204
Amount: 100 µl
Price: $370.00
Cat. No. 171 204 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not recommended
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 500 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunogen Recombinant protein corresponding to AA 763 to 848 from mouse Neurofilament M (UniProt Id: P08553)
Reactivity Reacts with: rat (P12839), mouse (P08553).
Other species not tested yet.
Data sheet 171_204.pdf

References for Neurofilament M - 171 204

Synaptic and functional alterations in the development of mutant huntingtin expressing hiPSC-derived neurons.
Dinamarca MC, Colombo L, Tousiaki NE, Müller M, Pecho-Vrieseling E
Frontiers in molecular biosciences (2022) 9: 916019. 171 204 ICC; tested species: human
Peptide backbone modifications of amyloid β (1-40) impact fibrillation behavior and neuronal toxicity.
Schwarze B, Korn A, Höfling C, Zeitschel U, Krueger M, Roßner S, Huster D
Scientific reports (2021) 111: 23767. 171 204 ICC; tested species: mouse
Neuroglia infection by rabies virus after anterograde virus spread in peripheral neurons.
Potratz M, Zaeck LM, Weigel C, Klein A, Freuling CM, Müller T, Finke S
Acta neuropathologica communications (2020) 81: 199. 171 204 IHC; tested species: mouse
Autophagy is essential for oligodendrocyte differentiation, survival, and proper myelination.
Bankston AN, Forston MD, Howard RM, Andres KR, Smith AE, Ohri SS, Bates ML, Bunge MB, Whittemore SR
Glia (2019) : . 171 204 IHC; tested species: mouse
Impaired Neurofilament Integrity and Neuronal Morphology in Different Models of Focal Cerebral Ischemia and Human Stroke Tissue.
Mages B, Aleithe S, Altmann S, Blietz A, Nitzsche B, Barthel H, Horn AKE, Hobusch C, Härtig W, Krueger M, Michalski D, et al.
Frontiers in cellular neuroscience (2018) 12: 161. 171 204 IHC; tested species: human,mouse
Cat. No.: 171 204
Amount: 100 µl
Price: $370.00
Synaptic and functional alterations in the development of mutant huntingtin expressing hiPSC-derived neurons.
Dinamarca MC, Colombo L, Tousiaki NE, Müller M, Pecho-Vrieseling E
Frontiers in molecular biosciences (2022) 9: 916019. 171 204 ICC; tested species: human
Peptide backbone modifications of amyloid β (1-40) impact fibrillation behavior and neuronal toxicity.
Schwarze B, Korn A, Höfling C, Zeitschel U, Krueger M, Roßner S, Huster D
Scientific reports (2021) 111: 23767. 171 204 ICC; tested species: mouse
Neuroglia infection by rabies virus after anterograde virus spread in peripheral neurons.
Potratz M, Zaeck LM, Weigel C, Klein A, Freuling CM, Müller T, Finke S
Acta neuropathologica communications (2020) 81: 199. 171 204 IHC; tested species: mouse
Autophagy is essential for oligodendrocyte differentiation, survival, and proper myelination.
Bankston AN, Forston MD, Howard RM, Andres KR, Smith AE, Ohri SS, Bates ML, Bunge MB, Whittemore SR
Glia (2019) : . 171 204 IHC; tested species: mouse
Impaired Neurofilament Integrity and Neuronal Morphology in Different Models of Focal Cerebral Ischemia and Human Stroke Tissue.
Mages B, Aleithe S, Altmann S, Blietz A, Nitzsche B, Barthel H, Horn AKE, Hobusch C, Härtig W, Krueger M, Michalski D, et al.
Frontiers in cellular neuroscience (2018) 12: 161. 171 204 IHC; tested species: human,mouse
Background

Neurofilaments (NFs) are intermediate filaments essential for providing structural support to neurons, particularly within axons. They play a crucial role in maintaining axonal diameter, which directly influences nerve conduction velocity (1). Neurofilaments are composed of three primary subunits - NF-L (light), NF-M (medium) and NF-H (heavy) – along with an NF-associated protein. In the adult central nervous system (CNS), α-internexin serves as the fourth neurofilament subunit, whereas in the peripheral nervous system (PNS), peripherin takes on this role (2).
Beyond their structural function, neurofilaments are also valuable biomarkers in both research and clinical settings. They are widely used in immunohistochemistry to stain and visualize axons, particularly in peripheral nerves and the CNS. Increased levels of neurofilament proteins in cerebrospinal fluid (CSF) or blood are strongly associated with neurodegenerative diseases, such as amyotrophic lateral sclerosis (ALS), multiple sclerosis (MS), and Alzheimer’s disease (3). In peripheral nerve studies, neurofilament staining is often combined with other markers, such as S100, to provide a more comprehensive assessment of nerve structure and pathology (4).