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Neurofilament H antibody - 171 121

Neurofilaments are major neuronal intermediate filaments
Mouse monoclonal purified IgG
Cat. No.: 171 121
Amount: 100 µg
Price: $415.00
Cat. No. 171 121 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP-staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 100 up to 1 : 1000 gallery  
Clone N52
Subtype IgG1
Immunogen Full length purified pig Neurofilament H (UniProt Id: F1RFH3)
Reactivity Reacts with: mouse (P19246), rat (P16884), pig (F1RFH3), ape, human (P12036).
Other species not tested yet.
Specificity Detects phosphorylated and unphosphorylated Neurofimanent H.
Data sheet 171_121.pdf

References for Neurofilament H - 171 121

Expression of neurofilaments and of a titin epitope in thymic epithelial tumors. Implications for the pathogenesis of myasthenia gravis.
Marx A, Wilisch A, Schultz A, Greiner A, Magi B, Pallini V, Schalke B, Toyka K, Nix W, Kirchner T, Müller-Hermelink HK, et al.
The American journal of pathology (1996) 1486: 1839-50. 171 121 WB, IHC; tested species: human
Reactivity of a panel of neurofilament antibodies on phosphorylated and dephosphorylated neurofilaments.
Shaw G, Osborn M, Weber K
European journal of cell biology (1986) 421: 1-9. 171 121 IHC, WB; tested species: human,rat
Comparative study of the three neurofilament subunits within pig and human retinal ganglion cells.
Ruiz-Ederra J, García M, Hicks D, Vecino E
Molecular vision (2004) 10: 83-92. 171 121 IHC; tested species: pig
Evidence that Wallerian degeneration and localized axon degeneration induced by local neurotrophin deprivation do not involve caspases.
Finn JT, Weil M, Archer F, Siman R, Srinivasan A, Raff MC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2000) 204: 1333-41. 171 121 IHC; tested species: mouse,rat
Accumulation of amyloid beta and tau and the formation of neurofilament inclusions following diffuse brain injury in the pig.
Smith DH, Chen XH, Nonaka M, Trojanowski JQ, Lee VM, Saatman KE, Leoni MJ, Xu BN, Wolf JA, Meaney DF
Journal of neuropathology and experimental neurology (1999) 589: 982-92. 171 121 IHC; tested species: pig
Expression of neurofilaments and of a titin epitope in thymic epithelial tumors. Implications for the pathogenesis of myasthenia gravis.
Marx A, Wilisch A, Schultz A, Greiner A, Magi B, Pallini V, Schalke B, Toyka K, Nix W, Kirchner T, Müller-Hermelink HK, et al.
The American journal of pathology (1996) 1486: 1839-50. 171 121 WB, IHC; tested species: human
Unexpected immunoreactivities of intermediate filament antibodies in human brain and brain tumors.
Franke FE, Schachenmayr W, Osborn M, Altmannsberger M
The American journal of pathology (1991) 1391: 67-79. 171 121 IHC; tested species: human
Evidence for a hepatocellular lineage in a combined hepatocellular-cholangiocarcinoma of transitional type.
Fisher HP, Doppl W, Osborn M, Altmannsberger M
Virchows Archiv. B, Cell pathology including molecular pathology (1988) 562: 71-6. 171 121 IHC; tested species: human
Reactivity of a panel of neurofilament antibodies on phosphorylated and dephosphorylated neurofilaments.
Shaw G, Osborn M, Weber K
European journal of cell biology (1986) 421: 1-9. 171 121 IHC, WB; tested species: human,rat
Cat. No.: 171 121
Amount: 100 µg
Price: $415.00
Expression of neurofilaments and of a titin epitope in thymic epithelial tumors. Implications for the pathogenesis of myasthenia gravis.
Marx A, Wilisch A, Schultz A, Greiner A, Magi B, Pallini V, Schalke B, Toyka K, Nix W, Kirchner T, Müller-Hermelink HK, et al.
The American journal of pathology (1996) 1486: 1839-50. 171 121 WB, IHC; tested species: human
Reactivity of a panel of neurofilament antibodies on phosphorylated and dephosphorylated neurofilaments.
Shaw G, Osborn M, Weber K
European journal of cell biology (1986) 421: 1-9. 171 121 IHC, WB; tested species: human,rat
Comparative study of the three neurofilament subunits within pig and human retinal ganglion cells.
Ruiz-Ederra J, García M, Hicks D, Vecino E
Molecular vision (2004) 10: 83-92. 171 121 IHC; tested species: pig
Evidence that Wallerian degeneration and localized axon degeneration induced by local neurotrophin deprivation do not involve caspases.
Finn JT, Weil M, Archer F, Siman R, Srinivasan A, Raff MC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2000) 204: 1333-41. 171 121 IHC; tested species: mouse,rat
Accumulation of amyloid beta and tau and the formation of neurofilament inclusions following diffuse brain injury in the pig.
Smith DH, Chen XH, Nonaka M, Trojanowski JQ, Lee VM, Saatman KE, Leoni MJ, Xu BN, Wolf JA, Meaney DF
Journal of neuropathology and experimental neurology (1999) 589: 982-92. 171 121 IHC; tested species: pig
Expression of neurofilaments and of a titin epitope in thymic epithelial tumors. Implications for the pathogenesis of myasthenia gravis.
Marx A, Wilisch A, Schultz A, Greiner A, Magi B, Pallini V, Schalke B, Toyka K, Nix W, Kirchner T, Müller-Hermelink HK, et al.
The American journal of pathology (1996) 1486: 1839-50. 171 121 WB, IHC; tested species: human
Unexpected immunoreactivities of intermediate filament antibodies in human brain and brain tumors.
Franke FE, Schachenmayr W, Osborn M, Altmannsberger M
The American journal of pathology (1991) 1391: 67-79. 171 121 IHC; tested species: human
Evidence for a hepatocellular lineage in a combined hepatocellular-cholangiocarcinoma of transitional type.
Fisher HP, Doppl W, Osborn M, Altmannsberger M
Virchows Archiv. B, Cell pathology including molecular pathology (1988) 562: 71-6. 171 121 IHC; tested species: human
Reactivity of a panel of neurofilament antibodies on phosphorylated and dephosphorylated neurofilaments.
Shaw G, Osborn M, Weber K
European journal of cell biology (1986) 421: 1-9. 171 121 IHC, WB; tested species: human,rat
Background
Neurofilaments are exclusively expressed in nerve cells and are the major structural component of large-diameter myelinated axons. They are predominatly composed of three proteins, Neurofilament H, L and M and are among the most highly phosphorylated neuronal proteins.