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Home > Products > 171 011

Neurofilament L antibody - 171 011

Neurofilaments are major neuronal intermediate filaments
Mouse monoclonal purified IgG
Cat. No.: 171 011
Amount: 100 µg
Price: $415.00
Cat. No. 171 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: 1 : 1000 gallery  
ExM: yes
DNA-PAINT: yes (see remarks)
Clone 69H10
Immunogen Recombinant protein corresponding to residues near the carboxy terminus of rat Neurofilament L. (UniProt Id: P19527)
Reactivity Reacts with: mouse (P08551), rat (P19527).
Other species not tested yet.
Remarks

DNA-PAINT: This antibody has been successfully used for DNA-PAINT application (see Unterauer et al., 2024; PMID: 38552614).
Data sheet 171_011.pdf

References for Neurofilament L - 171 011

Enhanced synaptic protein visualization by multicolor super-resolution expansion microscopy.
Eilts J, Reinhard S, Michetschläger N, Werner C, Sauer M
Neurophotonics (2023) 104: 044412. 171 011 ExM; tested species: mouse
Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 171 011 DNA-PAINT; tested species: rat
Cat. No.: 171 011
Amount: 100 µg
Price: $415.00
Enhanced synaptic protein visualization by multicolor super-resolution expansion microscopy.
Eilts J, Reinhard S, Michetschläger N, Werner C, Sauer M
Neurophotonics (2023) 104: 044412. 171 011 ExM; tested species: mouse
Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 171 011 DNA-PAINT; tested species: rat
WB
ICC
IHC
IHC
IHC-P
IHC-P
IHC-P
Background
Neurofilaments are exclusively expressed in nerve cells and are the major structural component of large-diameter myelinated axons. They are predominatly composed of three proteins, Neurofilament H, L and M and are among the most highly phosphorylated neuronal proteins.
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