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Home > Products > 166 103

ZnT1 antibody - 166 103 K.D.

ZnT1 is an abundantly expressed Zinc-transporter involved in the efflux of Zn2+
Rabbit polyclonal purified antibody
Cat. No.: 166 103
Amount: 50 µg
Price: $380.00
Cat. No. 166 103 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: not tested yet
IHC-P: 1 : 1000 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Synthetic peptide corresponding to AA 494 to 507 from rat ZnT1 (UniProt Id: Q62720)
Reactivity Reacts with: rat (Q62720), mouse (Q60738).
Other species not tested yet.
Specificity K.D. validated PubMed: 34871934
Data sheet 166_103.pdf

References for ZnT1 - 166 103

Modulation of ZnT-1 by Let7a unveils a therapeutic potential in amyotrophic lateral sclerosis.
Anzilotti S, Franco C, Valsecchi V, Cuomo O, Lombardi G, Di Muraglia N, De Iesu N, Laudati G, Annunziato L, Canzoniero LMT, Giuseppe P, et al.
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics (2025) : e00571. 166 103 WB; KD verified; tested species: mouse
ZnT1 is a neuronal Zn2+/Ca2+ exchanger.
Gottesman N, Asraf H, Bogdanovic M, Sekler I, Tzounopoulos T, Aizenman E, Hershfinkel M
Cell calcium (2021) 101: 102505. 166 103 WB; KD verified; tested species: mouse
Cat. No.: 166 103
Amount: 50 µg
Price: $380.00
Modulation of ZnT-1 by Let7a unveils a therapeutic potential in amyotrophic lateral sclerosis.
Anzilotti S, Franco C, Valsecchi V, Cuomo O, Lombardi G, Di Muraglia N, De Iesu N, Laudati G, Annunziato L, Canzoniero LMT, Giuseppe P, et al.
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics (2025) : e00571. 166 103 WB; KD verified; tested species: mouse
ZnT1 is a neuronal Zn2+/Ca2+ exchanger.
Gottesman N, Asraf H, Bogdanovic M, Sekler I, Tzounopoulos T, Aizenman E, Hershfinkel M
Cell calcium (2021) 101: 102505. 166 103 WB; KD verified; tested species: mouse
WB
ICC
ICC
IHC-P
IHC-P
IHC-P
IHC-P
IHC-P
Background

The essential micronutrient zinc (Zn2+) plays an important role in many biological processes like growth, development, and reproduction. It is found in the active site of many enzymes, where ionization, polarization or replacement of Zn2+ bound water is involved in catalytic reactions. As a charged ion Zn2+ cannot cross biological membranes by simple diffusion and must be transported by specialized transport mechanisms. Two families of Zn2+ transporters, SLC30 (ZnT, Zn2+ transporter) and SLC39 (ZIP, Zinc (Zn2+)-Iron (Fe2+) permease), function in opposite directions to maintain cellular Zn2+ homeostasis (1).
Ten Zn2+ transporter proteins ZnT1-10 have been described. All of them contain several transmembrane domains and a histidine rich intracellular loop (2).
ZnT1 is abundantly expressed and has been suggested to be responsible for the efflux of Zn2+ from cells. It is upregulated by high oral Zn2+ doses. ZnT1 plays an essential role in maintaining neuronal Zn2+ balance, which is critical for synaptic signalling, neuroprotection, and the prevention of Zn2+ toxicity. Dysregulation of ZnT1 in neural tissues has been linked to neurodegenerative conditions and cognitive impairments (3). ZnT1 is involved in regulating Zn2+ levels within β-cells, which is essential for insulin storage and secretion. Proper ZnT1 function ensures optimal pancreatic endocrine activity and supports glucose homeostasis (4).