Synaptic Systems

Cat. No.: 165 005

Amount: 50 µg

Price: $460.00

Cat. No. 165 005	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: yes IHC-P: 1 : 200 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 2 to 185 from mouse Vti1a (UniProt Id: O89116)
Reactivity	Reacts with: rat (Q9JI51), mouse (O89116). Other species not tested yet.
Specificity	K.O. validated
Matching control protein/peptide	165-0P
Data sheet	165_005.pdf

References for Vti1a - 165 005

Mapping localization of 21 endogenous proteins in the Golgi apparatus of rodent neurons.
van Bommel DM, Toonen RF, Verhage M
Scientific reports (2023) 131: 2871. 165 005 ICC; tested species: mouse

Control of Insulin Granule Formation and Function by the ABC Transporters ABCG1 and ABCA1 and by Oxysterol Binding Protein OSBP.
Hussain SS, Harris MT, Kreutzberger AJB, Inouye CM, Doyle CA, Castle AM, Arvan P, Castle JD
Molecular biology of the cell (2018) : . 165 005 ICC; tested species: rat

Cat. No.: 165 005

Amount: 50 µg

Price: $460.00

ICC

IHC-P

Background

Vti1a and Vti1b are mammalian SNARE proteins which have been identified as homologs of the yeast Vtip protein which is part of several SNARE complexes involved in transport.
Vti1a interacts with the cis-Golgi t-SNARE syntaxin 5 and the trans-Golgi network SNAREs syntaxin 6, syntaxin 16 and vamp 4.
Recently a brain-specific splice variant of Vti1a has been described. This Vti1a-β protein is associated with small synaptic vesicles, clathrin coated vesicles and endosomes. It is part of a SNARE complex different from the synaptic exocytotic compex since it does not co-immunoprecipitate with syntaxin 1 or SNAP 25. It is composed of syntaxin 6, syntaxin 16, vamp 4 and Vti1a-β which may play a role in biogenesis and/or recycling of synaptic vesicles. Nevertheless it behaves like a typical SNARE complex and can bind NSF and α/β-SNAP.

1 ) Siddiqi SA et al., J. Biol. Chem. (2006)
2 ) Brandhorst D et al., Proc. Natl. Acad. Sci. U.S.A. (2006)
3 ) Bose A et al., J. Biol. Chem. (2005)
4 ) Mallard F et al., J. Cell Biol. (2002)
5 ) Kreykenbohm V et al., Eur. J. Cell Biol. (2002)
6 ) Antonin W et al., J. Neurosci. (2000)

Protocols

Standard protocol

ICC

IHC

IHC-P

OTHER

Preabsorbtion

Other products for this target

Vti1a antibody - 165 005 K.O.

References for Vti1a - 165 005