Synaptic Systems

Cat. No.: 165 002

Amount: 200 µl

Price: $355.00

Cat. No. 165 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 2 to 185 from mouse Vti1a (UniProt Id: O89116)
Reactivity	Reacts with: rat (Q9JI51), mouse (O89116). Other species not tested yet.
Specificity	K.O. validated
Matching control protein/peptide	165-0P
Data sheet	165_002.pdf

References for Vti1a - 165 002

The double deficiency of the SNARE proteins vti1a and vti1b affects neurite outgrowth and signaling in N1E-115 neuroblastoma cells.
Kotschnew K, Winkler D, Reckmann J, Mann C, Schweigert A, Tellkamp G, Müller KM, Fischer von Mollard G
European journal of cell biology (2024) 1034: 151461. 165 002 WB; tested species: mouse

Lysosomal exocytosis releases pathogenic α-synuclein species from neurons in synucleinopathy models.
Xie YX, Naseri NN, Fels J, Kharel P, Na Y, Lane D, Burré J, Sharma M
Nature communications (2022) 131: 4918. 165 002 WB; tested species: mouse

VAMP4 maintains a Ca2+-sensitive pool of spontaneously recycling synaptic vesicles.
Lin PY, Chanaday NL, Horvath PM, Ramirez DMO, Monteggia LM, Kavalali ET
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 165 002 WB; tested species: rat

Annexin A6 and Late Endosomal Cholesterol Modulate Integrin Recycling and Cell Migration.
García-Melero A, Reverter M, Hoque M, Meneses-Salas E, Koese M, Conway JR, Johnsen CH, Alvarez-Guaita A, Morales-Paytuvi F, Elmaghrabi YA, Pol A, et al.
The Journal of biological chemistry (2016) 2913: 1320-35. 165 002 WB

Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 165 002 WB

Calsyntenin-1 shelters APP from proteolytic processing during anterograde axonal transport.
Steuble M, Diep TM, Schätzle P, Ludwig A, Tagaya M, Kunz B, Sonderegger P
Biology open (2012) 18: 761-74. 165 002 WB

The COG complex interacts directly with Syntaxin 6 and positively regulates endosome-to-TGN retrograde transport.
Laufman O, Hong W, Lev S
The Journal of cell biology (2011) 1943: 459-72. 165 002 WB, ICC

Dual roles of the mammalian GARP complex in tethering and SNARE complex assembly at the trans-golgi network.
Pérez-Victoria FJ, Bonifacino JS
Molecular and cellular biology (2009) 2919: 5251-63. 165 002 WB

Proteomic analysis reveals the composition of glutamatergic organelles of auditory inner hair cell.
Cepeda AP, Ninov M, Neef J, Parfentev I, Kusch K, Reisinger E, Jahn R, Moser T, Urlaub H
Molecular & cellular proteomics : MCP (2023) : 100704. 165 002 IHC; tested species: mouse

Cat. No.: 165 002

Amount: 200 µl

Price: $355.00

ICC

IHC

Background

Vti1a and Vti1b are mammalian SNARE proteins which have been identified as homologs of the yeast Vtip protein which is part of several SNARE complexes involved in transport.
Vti1a interacts with the cis-Golgi t-SNARE syntaxin 5 and the trans-Golgi network SNAREs syntaxin 6, syntaxin 16 and vamp 4.
Recently a brain-specific splice variant of Vti1a has been described. This Vti1a-β protein is associated with small synaptic vesicles, clathrin coated vesicles and endosomes. It is part of a SNARE complex different from the synaptic exocytotic compex since it does not co-immunoprecipitate with syntaxin 1 or SNAP 25. It is composed of syntaxin 6, syntaxin 16, vamp 4 and Vti1a-β which may play a role in biogenesis and/or recycling of synaptic vesicles. Nevertheless it behaves like a typical SNARE complex and can bind NSF and α/β-SNAP.

1 ) Siddiqi SA et al., J. Biol. Chem. (2006)
2 ) Brandhorst D et al., Proc. Natl. Acad. Sci. U.S.A. (2006)
3 ) Bose A et al., J. Biol. Chem. (2005)
4 ) Mallard F et al., J. Cell Biol. (2002)
5 ) Kreykenbohm V et al., Eur. J. Cell Biol. (2002)
6 ) Antonin W et al., J. Neurosci. (2000)

Protocols

Standard protocol

ICC

IHC

OTHER

Preabsorbtion

Other products for this target

Vti1a antibody - 165 002 K.O.

References for Vti1a - 165 002